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과학기술 정책전문가 및 위원회 관리를 위한 정보시스템 연구

박상은(Edberg S. Bak),윤영준(Young Joon Yoon),신성호(Sung Ho Shin),조항석(HangSuk Cho),손강렬(Kang-ryul Shon) 한국콘텐츠학회 2007 한국콘텐츠학회 종합학술대회 논문집 Vol.5 No.2_1
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과학기술 정책 입안 과정에서 정부 및 공공기관에서는 다양한 분야의 과학, 기술 및 정책 전문가를 필요로 한다. 이러한 전문가를 선정하는데 있어서 선정의 공정성, 다양성 그리고 선정자의 전문성을 보장하는 것이 매우 중요하다고 할 수 있다. 본 연구는 이러한 과학기술 정책 전문가 인력 데이터베이스를 관리하고 나아가 과학기술 정책 전문가를 과학기술 관련 위원회에 추천하고, 해당 위원회의 구성, 위원 선정 및 위원회 개최의 관리 등에 걸친 전반적인 범위의 서비스를 시스템적으로 접근하는 방법론을 대상으로 한다. For the decision making of governmental policy about science and technology, domain expert is needed. To choose the expert, the fairness, diversity and speciality is very important. In this research, systematical approach is proposed in the establishment and management of expert database, and recommendation of member of committee for specific field. Additionally, this approach includes the development of tools for the management of governmental committee and the constituents.
2
3D Detection of Mutant Hairs in Drosophila Wing Images

Jihoon Kwak,Chun-Taek Oh,Sung-Jun Han,Michael Adsetts Edberg Hansen,Auguste Genovesio,Myungjoo Kang 한국산업응용수학회 2011 한국산업응용수학회 학술대회 논문집 Vol.6 No.1
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We developed an automated mutant hair counting system in Drosophila wing images. In our previous work [2], we developed the image acquisition method using multi-focused image stack, hair separation method into upper and lower hair, and hair detection method using template matching. In this work, the hair detection and mutant classification algorithms are enhanced and extended to 3D, and the wing area segmentation is newly developed.
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Preferential Binding to Elk-1 by SLE-Associated IL10 Risk Allele Upregulates IL10 Expression

Sakurai, Daisuke,Zhao, Jian,Deng, Yun,Kelly, Jennifer A.,Brown, Elizabeth E.,Harley, John B.,Bae, Sang-Cheol,Alarcό,n-Riquelme, Marta E.,Edberg, Jeffrey C.,Kimberly, Robert P.,Ramsey-Goldman, Ros Public Library of Science 2013 PLoS genetics Vol.9 No.10
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<▼1>Immunoregulatory cytokine interleukin-10 (IL-10) is elevated in sera from patients with systemic lupus erythematosus (SLE) correlating with disease activity. The established association of IL10 with SLE and other autoimmune diseases led us to fine map causal variant(s) and to explore underlying mechanisms. We assessed 19 tag SNPs, covering the IL10 gene cluster including IL19, IL20 and IL24, for association with SLE in 15,533 case and control subjects from four ancestries. The previously reported IL10 variant, rs3024505 located at 1 kb downstream of IL10, exhibited the strongest association signal and was confirmed for association with SLE in European American (EA) (P = 2.7×10−8, OR = 1.30), but not in non-EA ancestries. SNP imputation conducted in EA dataset identified three additional SLE-associated SNPs tagged by rs3024505 (rs3122605, rs3024493 and rs3024495 located at 9.2 kb upstream, intron 3 and 4 of IL10, respectively), and SLE-risk alleles of these SNPs were dose-dependently associated with elevated levels of IL10 mRNA in PBMCs and circulating IL-10 protein in SLE patients and controls. Using nuclear extracts of peripheral blood cells from SLE patients for electrophoretic mobility shift assays, we identified specific binding of transcription factor Elk-1 to oligodeoxynucleotides containing the risk (G) allele of rs3122605, suggesting rs3122605 as the most likely causal variant regulating IL10 expression. Elk-1 is known to be activated by phosphorylation and nuclear localization to induce transcription. Of interest, phosphorylated Elk-1 (p-Elk-1) detected only in nuclear extracts of SLE PBMCs appeared to increase with disease activity. Co-expression levels of p-Elk-1 and IL-10 were elevated in SLE T, B cells and monocytes, associated with increased disease activity in SLE B cells, and were best downregulated by ERK inhibitor. Taken together, our data suggest that preferential binding of activated Elk-1 to the IL10 rs3122605-G allele upregulates IL10 expression and confers increased risk for SLE in European Americans.</▼1><▼2>Author SummarySystemic lupus erythematosus (SLE), a debilitating autoimmune disease characterized by the production of pathogenic autoantibodies, has a strong genetic basis. Variants of the IL10 gene, which encodes cytokine interleukin-10 (IL-10) with known function of promoting B cell hyperactivity and autoantibody production, are associated with SLE and other autoimmune diseases, and serum IL-10 levels are elevated in SLE patients correlating with increased disease activity. In this study, to discover SLE-predisposing causal variant(s), we assessed variants within the genomic region containing IL10 and its gene family member IL19, IL20 and IL24 for association with SLE in case and control subjects from diverse ancestries. We identified SLE-associated SNP rs3122605 located at 9.2 kb upstream of IL10 as the most likely causal variant in subjects of European ancestry. The SLE-risk allele of rs3122605 was dose-dependently associated with elevated IL10 expression at both mRNA and protein levels in peripheral blood samples from SLE patients and controls, which could be explained, at least in part, by its preferential binding to Elk-1, a transcription factor activated in B cells during active disease of SLE patients. Elk-1-mediated IL-10 overexpression could be downregulated by inhibiting activation of mitogen-activated protein kinases, suggesting a potential therapeutic target for SLE.</▼2>

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