http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans.
Kim, Hee Taek,Ko, Hyeok-Jin,Kim, Nahyun,Kim, Duwoon,Lee, Dongho,Choi, In-Geol,Woo, Hee Chul,Kim, Myoung Dong,Kim, Kyoung Heon Kluwer Academic Publishers 2012 Biotechnology letters Vol.34 No.6
<P>A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by 관-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50°C and pH 7 and was slightly increased in the presence of Na(+). The K(M), V(max), k(cat), and k(cat)/K(M) values were: 3 mg ml(-1), 6.2 U mg(-1), 1.9 ?? 10(-2) s(-1), and 6.3 ?? 10(-3) mg(-1 )ml s(-1), respectively.</P>
Kim, Duwoon,Baik, Keun Sik,Hwang, Ye Seul,Choi, Jong-Soon,Kwon, Joseph,Seong, Chi Nam International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.10
<P>An alginate lyase-producing bacterium, designated AlyHP32<SUP>T</SUP>, was isolated from the gut of sea urchin (<I>Hemicentrotus pulcherrimus</I>) obtained from the South Sea, Republic of Korea. Cells of strain AlyHP32<SUP>T</SUP> were Gram-reaction-negative and motile with a single polar flagellum. The strain grew with 1–6 % (w/v) NaCl (optimum 2–4 %) and at 4–30 °C (optimum 15–25 °C). Phylogenetic analysis based on sequences of the 16S rRNA gene and five housekeeping genes (<I>atpA</I>, <I>pyrH</I>, <I>recA</I>, <I>rpoA</I> and <I>rpoD</I>) revealed that strain AlyHP32<SUP>T</SUP> belonged to the genus <I>Vibrio</I> and formed a compact clade with the <I>Vibrio splendidus</I> group. However, DNA–DNA hybridization and fingerprints using the repetitive primers BOX and REP indicated that strain AlyHP32<SUP>T</SUP> was distinct from closely related species of the genus <I>Vibrio</I>. The major fatty acids were summed feature 3 (C<SUB>16:1</SUB>ω7<I>c</I> and/or C<SUB>16:1</SUB>ω6<I>c</I>) and C<SUB>16:0</SUB>. The DNA G+C content was 44.1 mol%. The predominant quinone was ubiquinone Q-8. Based on genotypic, phenotypic and DNA–DNA hybridization analysis, strain AlyHP32<SUP>T</SUP> represents a novel species of the genus <I>Vibrio</I>; the name <I>Vibrio</I> <I>hemicentroti</I> sp. nov. (type strain AlyHP32<SUP>T</SUP> = KCTC 32085<SUP>T</SUP> = DSM 26178<SUP>T</SUP>) is proposed for this novel taxon.</P>
Lee, Joongjae,Kim, Inyong,Yeo, Seoungsoon,Kim, Duwoon,Kim, Misook KOREAN SOCIETY OF FOOD SCIENCE AND NUTRITION 2018 PREVENTIVE NUTRITION AND FOOD SCIENCE Vol.23 No.1
<P>Lysozyme is well known as a natural antimicrobial agent, but its function is limited in that it only combats Gram-positive bacteria. We investigated the inhibitory effects of dextran-conjugated lysozymes (DLs) against some strains of Gram-negative bacteria and viral hemorrhagic septicemia virus (VHSV). The Maillard reactions of the DL were performed at various pHs (3.0, 7.0, and 8.5) in the presence of saturated KBr solution for 1, 3, 5, 7, and 9 days. The growth inhibition effects against Gram-negative bacterial strains, such as <I>Escherichia coli</I>, <I>Vibrio parahaemolyticus</I>, <I>Pseudomonas aeruginosa</I>, <I>Shigella sonnei</I>, and <I>Shigella flexneri</I> were found only in some DLs. DLs incubated at pH 7.0 for 9 days strongly inhibited growth of <I>S. sonnei</I> (92.9%). Fathead minnow (FHM) cells were infected with VHSV. DLs treated at all pHs for 1 day resulted in more than 80% viability of VHSV-infected FHM cells. The results of this study suggest that our DLs can be useful in food preservatives, pharmaceuticals, or aquatic feed due to their inhibitory effects against pathogenic bacteria and viral infections.</P>