비공생 헤모글로빈 유전자 (RsHb gene)를 이용한 상추의 형질전환

조동욱,정규환 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1

A non-symbiotic hemoglobin gene from radish (Raphanus sativus L. var. sarivus) was cloned into the plant binary vector, pBI121, harboring CaMV 35S promoter and a neomycine phosphotranferase Ⅱ (NPT Ⅱ) gene. Cotyledon segments of lettuce "Chung Chi Ma (Lactuca sativa L. cv. Chungchima)" were infected with Agrobacterium tumefaciens LBA4404 containing pBI121 harboring a non-symbiotic hemoglobin gene (RsHb hemoglobin gene). Kanamycin-resistant shoots were obtained on selection medium containing 25㎎/L kanamycin. Induced shoots were rooted in selection medium containing 50㎎/L kanamycin. Regenerated lettuce were cultivated and selfed, and seeds were harvested in the green house. Transformation of the non-symbiotic hemoglobin gene into lettuce was confirmed by polymerase chain reaction (PCR) analysis with genomic DNA. A PCR fragment of 680 bp was obtained from the transformed lettuce. Inheritance of the non-symbiotic hemoglobin gene in lettuce T1 progeny was conducted by PCR analysis. Segregation ratio for the non-symbiotic hemoglobin gene was detected by PCR analysis and observed in 1:1 ratio.

무기계 항균제(Ag-ZA)의 항균력에 관한 조사

조덕제,정숙현,박동근,신춘환 東西大學校 1996 동서논문집 Vol.2 No.1

Zeolite-Na에 Ag이온을 치환시켜 제조한 무기계 항균제를 이용하여 식품의 부패나 식중독을 초래할 수 있는 5종의 세균에 대한 항균력을 조사하였다. 이 들 5종에 대한 최소 저해 농도는 Escherichia coli는 0.1%이상, Pseudomonas aeruginosa는 0.01%, Klebsiella pneumoniae는 0.05%, Staphylococcus aureus와 Bacillus subtlis는 0.03%로 나타났다. 이 들 세균에 대한 0.1%의 Ag-ZA 첨가에서의 살균효과는 90%의 균 사멸시간으로 나타낼 때 Escherichia coli는 70분, Bacillus subtlis는 92분, Staphylococcus aureus는 111분, Klebsiella pneumoniae는 158분, Pseudomonas aeruginosa는 231분으로 나타났고, Ag-ZA 첨가량의 증가에 따른 사멸 속도의 변화는 Gram 음성균에서는 속도의 증가가 나타나지만 Gram 양성균에서는 속도의 변화를 관찰할 수 없었다. The investigation of antimicrobial activity on the Ag-ZA which is prepared by ion exchanging between Zeolite-Na and Ag ion. Minimum inhibitory concentrations are 0.01% for Escherichia coli and Pseudomonas aeruginosa, 0.05% for Klebsiella pneumoniae and 0.03% for Staphylococcus aureus and Bacillus subtlis. 90% sterilization time with 0.1% Ag-ZA are 70min., 92min., 111min., 158min., 231min. for Escherichia coli, Bacillus subtlis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa respectively. The sterilization effect is more important in gram neative bacteria than gram positive bacteria.

구강암의 치료 후 생존율에 영향을 주는 인자에 대한 후향적 연구

조병호,민승기,오승환,이동근,김용각 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

The objective of this research is to find out the influencing factors to recur oral cancers. The outcomes of 85 patients(male: 62 patients, female: 23 patients) with oral cancer who were not treated any pre-operative radiotherapy, chemotherapy or previous oral surgery, other than routine dento-alveolar procedures and a recent diagnostic biopsy were analysed retrospectively. Actuarial (life tables) survival analysis showed the 5 year survival rate was 58%, 52% for male patients, 72% for female patients. The patients who were under 50 years old showed 71% of 5 year survival rate, 51% of 5 year survival rate were above 51 years old group. Patients who were treated with elective neck dissection showed better prognosis which were shown 73% of 5 year survival rate than not neck dissection which were reveal 45% of 5 year survival rate. In TNM stage of patients, advanced stage showed worse prognosis, but only N stage did not suggest to judge good prognosis. Most patients were treated under postoperative radiation therapy and showed better prognosis.

변단면 고층건물의 변동풍압특성

조지은,김동환,김종수,김영문 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Nowadays the assessment of structural safety and serviceability of a tall building during strong wind is one of the important design criteria. The purpose of this study is to predict the pressure fluctuation of the tall building have different taper ratio of 5%, 10%, 15%. According to taper ratio, to know that the effect of vibration control on a tapered tall building attacked across wind. And also to give basic concepts of the tapered building on unpredictable wind directions. Acquired pressure data allowed analysis of the mean and r.m.s pressure coefficient, power spectral density function in measured taps at different levels and columns with considering the effects of wind directions and boundary layer conditions.

播種量 差異에 따른 濟州在來 동부의 生育反應, 收量 및 粗成分 變化

趙南棋,金東賢,宋昌吉,劉哲受,高東煥 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

ABSTRACT This study was accomplished to look into the response of main growth characters, yield and chemical composition of Cheju local cowpea on Cheju Island based on the difference between seeding rate. The study took place between May and September, 1998. Number, of days to flowering tended to be delayed as the seeding rate increased. Plant length was the longest at 6kg/10a treatment and the next one was at 5kg/10a treatment. The shortest came from 2kg/10a treatment, 275 cm, 271 and 220cm respectively. Number of branchs and stem diameter became weaker as the seeding rate increased. Fresh forage yields per lOa was the heaviest which weighed 6,073kg at 6kg/10a treatment and 5,840kg at 5kg/10a treatment respectively. The rest decreased gradually. Dry matter yields per lOa, crude protein yields per lOa and total digestible nutrient yields per lOa showed a similar tendency to fresh yields per lOa. The percentage of crude protein and crude fat got higher as the seeding rate increased, but the percentage of crude ash and crude fiber rather tended to decrease. Nitrogen free extract and total digestible nutrient percentages were similar to the change of crude protein percentage. SPAD reading values were the highest at 6kg/10a and 5kg/10a treatment.

유한요소법을 이용한 판 구조물의 진동인텐시티 해석

조대승,이동환,김사수,정상민 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.57 No.-

구조물의 진동인텐시티를 파악하면 진동에너지 전달경로와 소산기구 및 주된 기지원의 위치 등을 규명할 수 있어 구조물 진동의 효율적 저감 대책을 수립할 수 있다. 본 연구에서는 선박, 해양구조물 등과 같은 대형 복잡한 구조물의 진동인텐시티를 유한요소법으로 해석할 경우 적정한 모텔링 기법을 마련하고자 평판과 보강판에 대해 유한요소 모텔링을 달리 하면서 진동인텐시티 해석을 수행하고, 그 결과를 고전적 근사해법인 assumed mode method에 의한 결과와 비교·검토하였다. 이로부터 유한요법을 이용하여 판 구조물의 진동인텐시티 크기 및 방향을 정확하게 산정하기 위해서는 고유진동 또는 강제진동 해석의 경우보다 세밀한 유한요소 모텔링이 요구되지만 진동에너지 전달경로 규명 등을 위한 정서적 해석은 강제진동해석을 정확하게 할 수 있는 정도의 유한요소 모텔로도 가능함을 확인하였다. 아울러, 두께가 다른 평판 구조물, L-자형 판 구조물 및 3차원 box-girder에 대한 진동인텔시티 해석을 수행하여 다양한 판 구조물에 있어서 진동에너지의 전달 현상을 고찰하였다. The interest in evaluation of structural intensity arises for practical reasons, because net energy flow distribution offers information of energy transmission path, positions of sources, and sinks of vibration energy. In this paper, structural intensity analysis of plate structures using finite element method(FEM), which can be applied for more complex structures, is carried out. The purpose of this analysis is to evaluate the relative accuracy according to mesh fineness. The structural intensity of unstiffened and stiffened plates varying their mesh fineness is analyzed and the results are compared with those obtained by the assumed mode method. As results, the proper mesh size in qualitative/ quantitative structural intrnsity analysis of plate structures is proposed. In addition, the propagation phenomenon of vibration energy is investigated for the thickness-varying flat plate, L-type plate, and box-girder structures.

천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

特性不安의 下位領域과 洋弓記錄과의 關係

조성환,이용인,김동건 忠南大學校體育科學硏究所 1994 體育科學硏究誌 Vol.12 No.1

Through 26 female archery players attending middle or high school in a psychologic unsettled period, we examine the effects of color and music on archery record. Then we come to a conclusion below. 1. There is no record difference in the statistics according to the hight of anxiety( =0.01 ) but strictly speaking low anxiety group shows better record than high anxiety group 2. There is a meaningful result in record difference by music according to anxiety and group with low anxiety shows higher record than group with high anxiety.( p〈0.01 ) 3. When various color is served, low anxiety group shows better record than high anxiety group. And the record by color by color is higher like this order ; white〉blue〉red〉yellow〉black. There is a big record difference between red and blue targets.( p〈0,01 ) 4. When we observe the record difference according to existence and nonexistence of music, we can see that nonexistence of music produce better record than existence of that.( p〈0.01 ) And the record gets lower when various color is provided.

言論의 不法行爲에 대한 法的硏究

曺日煥,李東勳 東義大學校法學硏究所 1989 공공정책연구 Vol.5 No.-

燐酸施肥量 差異가 靑刈豌豆의 主要形質 및 收量에 미치는 影響

趙男棋,韓英明,朴良門,高東煥 濟州大學校 亞熱帶農業硏究所 1998 亞熱帶農業硏究 Vol.15 No.-

本 硏究는 濟州道에서 燐酸施肥量差異가 靑刈豌豆의 主要 形質과 收量에 미치는 影響을 究明하기 위하여 濟州在來豌豆와 Sparkle을 供試하여 1998年 3月부터 5月까지 修行하였으며 試驗한 結果를 要約하면 다음과 같다. 1. 開花日數는 燐酸施肥量이 많아짐에 따라 늦어지는 傾向이었다. 品種問의 開花日數는 濟州在來豌豆가 빨랐고, Sparkle은 늦은 편이었다. 2. 草長은 燐酸施肥量이 많을수록 길었으며, 品種間에는 各 處理區에서 濟州在來豌豆가 길었고, Sparkle은 짧았다. 3. 莖直經, 主莖節數는 濟州在來豌豆 Sparkle 두 品種 供히 燐酸施肥量이 增加할수록 增加하였다. 4. 10a當 靑刈收量은 두 品種 모두 燐酸施肥量이 많아짐에 따라 漸次的으로 增加되는 傾向이었다. 5. 濟州求來談臺의 草長과 靑刈收量과는 高度로 有意한 正의 相關을, Sparkle은 正의 相關關係가 있어서 두 品種 모두가 草長이 길수록 靑刈收量이 많았다. Two pea cultivars, 'Cheju local pea' and 'Sparkle', were planted on 3 March at Cheju at 5 phosphate rates(0, 5, 10, 15, 20kg/10a) to determine the optimum phosphate fertilization rate. Days to flowering increased as phosphate rate was increased. Days to flowering of Cheju local pea was earlier than that of Sparkle. The more phosphate rate, the higher plant height Plant height of cultivar Cheju local pea was taller than of Sparkle. As phosphate rate was increased, stem diameter and number of nodes per plant of two cultivars increased. Fresh forage yield of two cultivars linearly increased as phosphate rate was increased from 0 to 20kg/10a. Plant height for each cultivar was positively correlated with fresh forage yield.