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Zhang, Dian-Chang,Shao, Yan-Qing,Huang, Yan-Qin,Jiang, Shi-Gui Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5
Cytokines are proteins produced by many different cells of the immune system and play a significant role in initiating and regulating the inflammatory process. In this research, an important cytokine, interleukin-10 (IL-10) gene, has been identified and characterized from zebrafish (Danio rerio) genome database. Zebrafish IL-10 is located within a 2690 bp fragment and contains five exons and four introns, sharing the same organization with mammalian IL-10 genes. An open reading frame of 543 bp was found to encode a putative 180 amino acid protein with a signal peptide of 22 amino acids, which shares 29.7-80.9% homology with amino acid sequences of other known IL-10. The signature motif of IL-10 is also conserved in zebrafish IL-10. The predicted transcript was finally confirmed by sequencing of cDNA clones. Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the tissue distribution and expression regulation of this gene in seven organs of normal and lipopolysaccharide (LPS) stimulation zebrafish. The results demonstrated that this gene was expressed slightly in normal kidney, gill and gut, no expression was detected in other four tissues. The expression was clearly upregulated after LPS stimulation. Using the ideal zebrafish model, further study of IL-10 characterization and function may provide insight on the understanding of the innate immune system.
Ren, Zi Yan,Zhang, Dian Hai,Pham, Minh Trien,Koh, Chang Seop Trans Tech Publications, Ltd. 2012 Materials science forum Vol.721 No.-
<P>A novel reliability calculation algorithm is developed and applied to a robust optimization of electromagnetic device with uncertain design variables taken into account. The proposed algorithm, to calculate the reliability of a given design, incorporates sensitivity analysis based on finite element method with Monte Carlo simulation (MCS) method so that it has higher numerical efficiency than conventional methods: MCS and first-order reliability methods. Through numerical examples, its applicability to a reliability-based robust optimal design of electromagnetic device is demonstrated.</P>
Optimization Design and Performance Analysis of Thomson-Coil Actuator by using Different Algorithms
렌지얀(Zi Van Ren),장전해(Dian Hai Zhang),리웨이(Wei Li),고창섭(Chang Seop Koh) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.10
In this paper, the optimization design and performance analysis of Thomson-coil actuator are implemented by using two different algorithms: enumeration algorithm and one global mixed-variable optimization algorithm based on the particle swarm optimization algorithm. The efficiency of these two methods are compared. Finally, the best design is obtained and corresponding dynamic performances are analysed.