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Highly sensitive turn-on biosensors by regulating fluorescent dye assembly on liposome surfaces
Seo, Sungbaek,Kwon, Min Sang,Phillips, Andrew W.,Seo, Deokwon,Kim, Jinsang The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.50
<P>We developed a new self-signaling sensory system built on phospholipid liposomes having H-aggregated R6G dyes on their surface. Selective molecular recognition of a target by the phospholipid displaces R6G from the liposome surface to turn on fluorescence signal. Selective and sensitive detection of neomycin down to 2.3 nM is demonstrated.</P> <P>Graphic Abstract</P><P>Herein, we developed a turn-on type liposome-based sensing platform by assembling fluorescence dyes to form H-type aggregation with emission quenching on phospholipid-liposome surfaces. Rationally devised specific interactions between the phospholipid and a target analyte effectively release the fluorescence dyes from H-aggregate, producing a turn-on signal. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc01621e'> </P>
Yang, Chungmo,Hwang, Hae Hyun,Jeong, Soohyun,Seo, Deokwon,Jeong, Yoon,Lee, Dong Yun,Lee, Kangwon Dove Medical Press 2018 International journal of nanomedicine Vol.13 No.-
<P><B>Purpose</B></P><P>Nitric oxide (NO) can be clinically applied at low concentrations to regulate angiogenesis. However, studies using small molecule NO donors (<I>N</I>-diazeniumdiolate, <I>S</I>-nitrosothiol, etc) have yet to meet clinical requirements due to the short half-life and initial burst-release profile of NO donors. In this study, we report the feasibility of methoxy poly(ethylene glycol)-b-poly(lactic-<I>co</I>-glycolic acid) (mPEG-PLGA) nanoparticles (NPs) as NO-releasing polymers (NO-NPs) for inducing angiogenesis.</P><P><B>Materials and methods</B></P><P>The mPEG–PLGA copolymers were synthesized by typical ring-opening polymerization of lactide, glycolide and mPEG as macroinitiators. Double emulsion methods were used to prepare mPEG–PLGA NPs incorporating hydrophilic NONOate (dieth-ylenetriamine NONOate).</P><P><B>Results</B></P><P>This liposomal NP encapsulates hydrophilic diethylenetriamine NONOate (70%±4%) more effectively than other previously reported materials. The application of NO-NPs at different ratios resulted in varying NO-release profiles with no significant cytotoxicity in various cell types: normal cells (fibroblasts, human umbilical vein endothelial cells and epithelial cells) and cancer cells (C6, A549 and MCF-7). The angiogenic potential of NO-NPs was confirmed in vitro by tube formation and ex vivo through an aorta ring assay. Tubular formation increased 189.8% in NO-NP–treated groups compared with that in the control group. Rat aorta exhibited robust sprouting angiogenesis in response to NO-NPs, indicating that NO was produced by polymeric NPs in a sustained manner.</P><P><B>Conclusion</B></P><P>These findings provide initial results for an angiogenesis-related drug development platform by a straightforward method with biocompatible polymers.</P>
Label-free bacterial detection using polydiacetylene liposomes
Park, Jimin,Ku, Seul Kathy,Seo, Deokwon,Hur, Kahyun,Jeon, Hojeong,Shvartsman, Dmitry,Seok, Hyun-Kwang,Mooney, David J.,Lee, Kangwon The Royal Society of Chemistry 2016 Chemical communications Vol.52 No.68
<P>Polydiacetylene (PDA) liposomes were prepared to selectively capture target released from bacteria. Specific interplay between released-surfactin and PDA resulted in a conformal change in the structure of PDA, highlighting the potential of indirect interactions between bacteria and PDA in the construction of new label-free bacterial sensors.</P>