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Dehong Feng,Junshan He,Chenyu Zhang,Ling Wang,Xiaofeng Gu,Yu Guo 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.9
Ameloblastoma in the tibia is rare. Limb reconstruction after tumor resection is challenging in terms of selection of the operativemethod. Here, we report a case of radical resection of an ameloblastoma in the mid-distal tibia combined with limb salvage usinga three-dimensional (3D)-printed prosthesis replacement, with 1-year follow-up results. After receiving local institutional reviewboard approval, a titanium alloy prosthesis was designed using a computer and manufactured with 3D-printing technology. Duringthe operation, the stem of the prosthesis was inserted closely into the proximal tibial medullary cavity. Then, the metal anklemortise and the talus were compacted closely. Radiographic results at 1-year follow up showed that the prosthesis was wellplaced, and no loosening was observed. The Musculoskeletal Tumor Society (MSTS) 93 functional score was 26 points, and thefunctional recovery percentage was 86.7%. Computer-assisted 3D-printing technology allowed for more volume and structuralcompatibility of the prosthesis, thereby ensuring a smooth operation and initial prosthetic stabilization. During the follow up, thepresence of bone ingrowths on the porous surface of some segments of the prosthesis suggested good outcomes for long-term biologicalintegration between the prosthesis and host bone.
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Dehong Xu,Xiao Sun,Wei Feng 전력전자학회 1998 ICPE(ISPE)논문집 Vol.- No.-
Based on the linear model of electromagnetic suspension (EMS) system, it is able to be further simplified into a standard second-order model with a modified PD control. In this paper static and dynamic characteristics of EMS with modified PD control are investigated when suspended weight of steel plate changes. A experimental system has been built to verify static and dynamic characteristics of EMS system. Simulation and experiment results are both given.<br/>
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Comparative genomic analysis of Mycoplasma anatis strains
Zhou Qi,Mai Kaijie,Yang Dehong,Liu Junfa,Yan Zhuanqiang,Luo Cuifen,Tan Yangtong,Cao Sheng,Zhou Qingfeng,Chen Li,Chen Feng 한국유전학회 2021 Genes & Genomics Vol.43 No.11
Background The Gram-negative intracellular bacterium Mycoplasma anatis is a pathogen of respiratory infectious diseases in ducks and has caused signifcant economic losses in the poultry industry. Objective This study, as the frst report of the structure and function of the pan-genome of Mycoplasma anatis, may provide a valuable genetic basis for many aspects of future research on the pathogens of waterfowl. Methods We sequenced the whole genomes of 15 Mycoplasma anatis isolated from ducks in China. Draft genome sequencing was carried out and whole-genome sequencing was performed by the sequencers of the PacBio Sequel and an IonTorrent Personal Genome Machine (PGM). Then the common genic elements of protein-coding genes, tRNAs, and rRNAs of Mycoplasma anatis genomes were predicted by using the pipeline Prokka v1.13.7. To investigate homologous protein clusters across Mycoplasma anatis genomes, we adopted Roary v3.13.0 to cluster orthologous genes (OGs) based on the following criteria. Results We obtained one complete genome and 14 genome sketches. Microbial mobile genetic element analysis revealed the distribution of insertion sequences (IS30, IS3, and IS1634), prophage regions, and CRISPR arrays in the genome of Mycoplasma anatis. Comparative genomic analysis decoded the genetic components and functional classifcation of the pan-genome of Mycoplasma anatis that comprised 646 core genes, 231 dispensable genes and among them 110 was strainspecifc. Virulence-related gene profles of Mycoplasma anatis were systematically identifed, and the products of these genes included bacterial ABC transporter systems, iron transport proteins, toxins, and secretion systems. Conclusion A complete virulence-related gene profle of Mycoplasma anatis has been identifed, most of the genes are highly conserved in all strains. Sequencing results are relevant to the molecular mechanisms of drug resistance, adaptive evolution of pathogens, population structure, and vaccine development.
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