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RISS 인기검색어
- 검색키워드
- 저자 : David G. Popovich (검색결과 3 건)
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David G. Popovich,Yiyu Lee,Lu Li,Wei Zhang 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.3
A triterpenoid containing bitter melon (Momordica charantia) seed (BMS) extract was found to reduce cultured 3T3-L1 cell viability. The 50% lethal concentration values were determined to be 0.78 ± 0.01 mg/mL at 24 hours, 0.69 ± 0.01 mg/mL at 48 hours, and 0.56 ± 0.02 mg/mL at 72 hours. 3T3-L1 cells were utilized as models of pre-adipocyte to adipocyte differentiation. BMS extract also caused a G_2/M arrest in the cell cycle reducing cells by 23.9%, 37.7%, and 34.7% compared with the control after 72 hours of treatment at concentrations of 0.4, 0.5, and 0.6 mg/mL respectively. BMS extract did not increase the release of lactate dehydrogenase from 3T3-L1 cells, which was unexpected. Furthermore, BMS extract reduced lipid accumulation during differentiation from pre-adipocyte to adipocyte corresponding to reduction in overall triglyceride of 32.4% after 72 hours compared with untreated control cells. BMS is an underutilized agricultural commodity that may have potential for nutraceutical and functional food development.
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Ginsenosides analysis of New Zealand-grown forest Panax ginseng by LC-QTOF-MS/MS
Chen, Wei,Balan, Prabhu,Popovich, David G. The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4
Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.
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Ginsenosides analysis of New Zealand – grown forest Panax ginseng by LC-QTOF-MS/MS
Wei Chen,Prabhu Balan,David G. Popovich 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4
Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides areaffected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer(P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem,and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterizedby Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified bymatching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginsengvaried, which was not obviously dependent on age. In the underground parts, the 13-year-old ginsengroot contained more abundant ginsenosides among tested ginseng samples, whereas in the abovegroundparts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount ofginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts ofP. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulatedP. ginseng.
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