Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function

Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

Critical Factors Affecting the Innovation Activities of Businesses: Evidence from Binh Dinh Province, Vietnam

NGUYEN, Thi Le Hang,PHAM, Ngoc Toan,DAO, Vu Phuong Linh,NGO, Thi Thanh Thuy,LE, Thi Thanh Binh Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.7

The study investigates the factors influencing the innovation activities in the enterprises in the Binh Dinh Province, Vietnam. By employing the dataset from a survey in 200 typical enterprises in Binh Dinh and using the Exploratory Factor Analysis and regression analysis, we found that there are eight factor groups affecting the innovation activities of enterprises. They include management of innovation promotion; market research capacity; leadership inspiring innovation; culture of innovation; human resources for implementing innovation; network connection; disseminating/sharing knowledge; and impact of producing/serving technology. All these factors strongly affect the innovation activities, which plays an important role in promoting the sustainable development of the Vietnamese enterprises, with the statistical significance level at 1%. Moreover, findings also show that, among these factors, the market research capacity is the strongest determinant of the innovation activity in the enterprise. An increase of 1 point of capacity of market research will increase the innovation activities in the enterprise by 0.114 point. It is followed by the management of promoting innovation, leadership inspiring innovation, and disseminating and sharing knowledge, with 0.104, 0.103 and 0.102 score, respectively. On the other hand, network connection is the weakest factor, with the score of 0.07 point.

Endocrine System on a Chip: a Model for Diabetes

DAO THI THUY NGUYEN,So Hyun KIM,Seunghan OH,Danny VAN NOORT,Sungsu PARK 한국생물공학회 2013 한국생물공학회 학술대회 Vol.2013 No.4

The Antecedents of Online Purchase Intention of Generation Z and Millennials toward Organic Cosmetics in an Emerging Economy

Thi Thu Ha Nguyen,Hiep-Hung Pham,Ngoc Linh Dao,Hong Hanh Dao,Thi Thuy Nuong Duong,Thi Ha Chu The International Academy of Global Business and T 2023 The International Academy of Global Business and T Vol.19 No.6

Purpose - Based on the extended theory of planned behavior (TPB), this study aims to investigate the antecedents of online purchase behavior toward organic cosmetics for Generation Z (Gen Z) and Millennials by focusing on the impact of subjective norms, health consciousness, trust, attitude, and intention. Design/Methodology/Approach - A survey method using an online questionnaire was adopted to collect data from 341 Gen Z and Millennial consumers in Vietnam. The collected data were then analyzed using structural equation modeling (SEM). Findings - The results of the study showed that, among antecedents, that attitude has the strongest impact on the purchase intention of online customers toward organic cosmetics, followed by trust and subjective norms. Interestingly, health consciousness has also been found to significantly impact Gen Z and Millennial online purchase intention toward organic cosmetics. Research Implications - This paper extends the academic literature on purchase intention toward green products. More importantly, to the best of the authors’ knowledge, this study is among the first to examine the antecedents of online purchase intention toward the organic cosmetics of Gen Z and Millennials in an emerging economy, Vietnam. The findings offer important implications for cosmetics companies, retailers, and other stakeholders to have deeper insights into factors influencing young Vietnamese consumer intentions toward purchasing organic beauty products online.

Microfluidic co-culture (intestinal and pancreatic tissues) device for screening anti-diabetes drugs

Dao THI THUY NGUYEN 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

A Comparative Study on the Adipogenic and Myogenic Capacity of Muscle Satellite Cells, and Meat Quality Characteristics between Hanwoo and Vietnamese Yellow Steers

Nguyen Thu Uyen,Dao Van Cuong,Pham Dieu Thuy,Luu Hong Son,Nguyen Thi Ngan,Nguyen Hung Quang,Nguyen Duc Tuan,In-ho Hwang 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.4

Myogenesis and adipogenesis are the important processes determining the muscle growth and fat accumulation livestock, which ultimately affecting their meat quality. Hanwoo is a popular breed and its meat has been exported to other countries. The objective of this study was to compare the myogenesis and adipogenesis properties in satellite cells, and meat quality between Hanwoo and Vietnamese yellow cattle (VYC). Same 28-months old Hanwoo (body weight: 728±45 kg) and VYC (body weight: 285±36 kg) steers (n=10 per breed) were used. Immediately after slaughter, tissue samples were collected from longissimus lumborum (LL) muscles for satellite cells isolation and assays. After 24 h post-mortem, LL muscles from left carcass sides were collected for meat quality analysis. Under the same in vitro culture condition, the proliferation rate was higher in Hanwoo compared to VYC (p<0.05). Fusion index was almost 3 times greater in Hanwoo (42.17%), compared with VYC (14.93%; p<0.05). The expressions of myogenesis (myogenic factor 5, myogenic differentiation 1, myogenin, and myogenic factor 6)- and adipogenesis (peroxisome proliferator-activated receptor gamma)-regulating genes, and triglyceride content were higher in Hanwoo, compared with VYC (p<0.05). Hanwoo beef had a higher intramuscular fat and total monounsaturated fatty acids contents than VYC beef (p<0.05). Whilst, VYC meat had a higher CIE a* and total polyunsaturated fatty acids content (p<0.05). Overall, there was a significant difference in the in vitro culture characteristics and genes expression of satellite cells, and meat quality between the Hanwoo and VYC.

Endocrine system on chip for a diabetes treatment model

Nguyen, Dao Thi Thuy,van Noort, Danny,Jeong, In-Kyung,Park, Sungsu IOP Publishing 2017 Biofabrication Vol.9 No.1

<P>The endocrine system is a collection of glands producing hormones which, among others, regulates metabolism, growth and development. One important group of endocrine diseases is diabetes, which is caused by a deficiency or diminished effectiveness of endogenous insulin. By using a microfluidic perfused 3D cell-culture chip, we developed an 'endocrine system on chip' to potentially be able to screen drugs for the treatment of diabetes by measuring insulin release over time. Insulin-secreting beta-cells are located in the pancreas, while L-cells, located in the small intestines, stimulate insulin secretion. Thus, we constructed a co-culture of intestinal-pancreatic cells to measure the effect of glucose on the production of glucagon-like peptide-1 (GLP-1) from the L-cell line (GLUTag) and insulin from the pancreatic beta-cell line (INS-1). After three days of culture, both cell lines formed aggregates, exhibited 3D cell morphology, and showed good viability (> 95%). We separately measured the dynamic profile of GLP-1 and insulin release at glucose concentrations of 0.5 and 20 mM, as well as the combined effect of GLP-1 on insulin production at these glucose concentrations. In response to glucose stimuli, GLUTag and INS-1 cells produced higher amounts of GLP-1 and insulin, respectively, compared to a static 2D cell culture. INS-1 combined with GLUTag cells exhibited an even higher insulin production in response to glucose stimulation. At higher glucose concentrations, the diabetes model on chip showed faster saturation of the insulin level. Our results suggest that the endocrine system developed in this study is a useful tool for observing dynamical changes in endocrine hormones (GLP-1 and insulin) in a glucose-dependent environment. Moreover, it can potentially be used to screen GLP-1 analogues and natural insulin and GLP-1 stimulants for diabetes treatment.</P>

A microfluidic enrichment platform with a recombinase polymerase amplification sensor for pathogen diagnosis

Dao, Thuy Nguyen Thi,Lee, Eun Yeong,Koo, Bonhan,Jin, Choong Eun,Lee, Tae Yoon,Shin, Yong Elsevier 2018 Analytical Biochemistry Vol.544 No.-

<P><B>Abstract</B></P> <P>Rapid and sensitive detection of low amounts of pathogen in large samples is needed for early diagnosis and treatment of patients and surveillance of pathogen. In this study, we report a microfluidic platform for detection of low pathogen levels in a large sample volume that couples an Magainin 1 based microfluidic platform for pathogen enrichment and a recombinase polymerase amplification (RPA) sensor for simultaneous pathogenic DNA amplification and detection in a label-free and real-time manner. Magainin 1 is used as a pathogen enrichment agent with a herringbone microfluidic chip. Using this enrichment platform, the detection limit was found to be 20 times more sensitive in 10 ml urine with <I>Salmonella</I> and 10 times more sensitive in 10 ml urine with <I>Brucella</I> than that of real-time PCR without the enrichment process. Furthermore, the combination system of the enrichment platform and an RPA sensor that based on an isothermal DNA amplification method with rapidity and sensitivity for detection can detect a pathogen at down to 50 CFU in 10 ml urine for <I>Salmonella</I> and 10<SUP>2</SUP> CFU in 10 ml urine for <I>Brucella</I> within 60 min. This system will be useful as it has the potential for better diagnosis of pathogens by increasing the capture efficiency of the pathogen in large samples, subsequently enhancing the detection limit of pathogenic DNA.</P>

Determinants of Implementation of Enterprise Resource Planning System: A Case Study in Vietnam

Xuan Hiep NGUYEN,Thi Cam Loan NGUYEN,Xuan Dao MAI,Thi To Loan BUI,Thi Tra Giang TRAN,Thuy Trinh HO,Thi Huyen NGUYEN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.4

Under the pressure of business competition and effectiveness, more and more organizations in the world are likely to apply modern software such as enterprise resource planning (ERP) which can help them to create better organizing practice, better managing capabilities of administrators, and better operations of personnel. In Vietnam, many firms are afraid to install it and the number of firms adopting this software is limited mainly due to the insufficient scientific evidence of the successful implementation of ERP. This study aims to identify and measure the factors influencing the successful implementation of ERP of firms in Vietnam. Qualitative research methodology combined with quantitative research methodology is used. The data are surveyed with 343 administrators of 45 organizations that have successfully deployed ERP in Ha Noi and Ho Chi Minh cities. Of these 19 were excluded from analyses due to unsatisfactory responses, and only 324 were used for statistical processing. The collected data were processed by using the SPSS software. The result of the study shows that there are six factors affecting the successful implementation of ERP of firms in Vietnam, (ranked in descending order of importance), they are top management commitment and support, business process re-engineering, user involvement, project management board, vendor support, and suitability of software and hardware.

Impact of Community Attachment and Resident's Support on Destination Sustainability: Evidence from Spiritual and Community Destination in Vietnam

THAN, Trong Thuy,KIEU, Thi Phuong Hoa,PHAM, Thi Anh Duong,HOANG, Thi Cam Van,TRAN, Thi Hau,NGUYEN, Huu Doan,DAO, Trung Kien Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8

This study investigates the influence of community involvement on the destination sustainability for community destinations and spiritual destinations in Vietnam. Community involvement is measured by two constructs, which are community attachment and residents' support. A structural questionnaire consisting of 41 observation variables measured on a 5-point Likert scale was used to survey households who live in a spiritual destination in An Giang province and three community destinations in Lang Son province with the help of local tour guides and Youth Union. 168 out of 200 responses collected were valid for multivariate data analysis. The results of confirmatory factor analysis (CFA) and structural equation modeling (SEM) reveal three main findings. Firstly, community attachment has a direct effect on both perceived benefits and destination sustainability. Secondly, while residents' support has a direct effect on perceived benefits, it indirectly affects destination sustainability. Finally, community destinations have a higher level of sustainability than the spiritual destination. Based on those findings, this study proposed three suggestions for local authorities, policymakers, and residents to improve the sustainable development of their local tourism destinations, including (1) diversifying local tourism products, (2) encouraging the community participation in tourism development programs, and (3) increasing in the expected benefits in local tourism development policies.