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Antioxidant and Antifungal Activity of Mixtures of Oligochitosan and Benzoic Analogues
( Dang Minh Chanh Nguyen ),( Dong Jun Seo ),( Woo Jin Jung ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.4
In this study, the differences between oligochitosan (MW 1,000~3,000 Da, C1K) and two compounds [Gallic acid (P-GA) and 3,4-dihydroxybenzoic acid (P-DHBA), Comps] purified from Terminalia nigrovenulosa bark (or two commercial compounds) and mixtures of C1K and Comps were investigated for their antioxidant activities (DPPH radical scavenging activity and total antioxidant capacity) and antifungal against Fusarium solani. The EC50 values of C1K for antifungal activity were in the range of 358.6-745.1 ppm. Both C1K and mixtures of C1K and Comps strongly inhibited mycelium growth of F. solani after 2 and 4 days incubation. Mixtures of C1K and Comps had high antioxidant activity in terms of both DPPH radical scavenging activity and total antioxidant capacity. These results suggested that mixtures of C1K and Comps triggered mechanism for the enhancement of suppressive activity to the phytopathogen F. solani and antioxidant activities.
( Dang Minh Chanh Nguyen ),( Dong Jun Seo ),( Ro Dong Park ),( Woo Jin Jung ) 한국응용생명화학회 2013 Journal of Applied Biological Chemistry (J. Appl. Vol.56 No.4
The nematicidal, antifungal and antioxidant activities of methanol extracts from six Vietnamese native medicinal plants were evaluated by various assays in vitro. Of the plant extracts tested, Terminalia nigrovenulosa was found to possess the highest activity when compared to the others. The leaves and bark of T. nigrovenulosa showed strong inhibitory activity against Meloidogyne incognita and Fusarium solani. The DPPH (1,1-Diphenyl-2- picryl-hydrazyl) radical scavenging, reducing power and total antioxidant activities of T. nigrovenulosa bark were higher than that of the remainder plant extracts. Chitinase activity of these plants was also investigated using SDS-PAGE. The results obtained in the present study indicate that T. nigrovenulosa leaf extracts are the greatest potential source as nematicides and fungicides for the control of M. incognita and F. solani. In particularly, T. nigrovenulosa bark extracts could be used as a potential source of commercially viable levels of natural antioxidant.
Effect of Chitosan Oligosaccharide and Plant Compounds on Plant Pathogenic Fungi
( Dang Minh Chanh Nguyen ),( Dong Jun Seo ),( Yong Su Song ),( Wan Taek Ju ),( Ro Dong Park ),( Woo Jin Jung ) 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.3
To investigate the antifungal activity of two phytopathogens, Rhizoctonia solani and Fusarium oxysporum in vitro, chitosan oligosaccharide (MW 1,000~3,000 Da, C1K), plant compounds [Gallic acid (P-GA) and 3,4-dihydroxybenzoic acid (P-DHBA), Comps] purified from Terminalia nigrovenulosa bark, and commercial compouds (C-GA and C-DHBA) were used in this study. Also, chitosan beads were used to make mixture of chitosan oligosacchade. The EC50 values of C1K were in the range of 3,438~3,450 ppm for R. solani and F. oxysporum, respectively. Mixtures of C1K and purified and commercial two Comps strongly inhibited within 65~78% mycelium growth of R. solani after 2 and 4 days incubation. Chitosan beads (CTB) combinded with C1K had high antifungal activity against R. solani. The EC50 values of CTB combinded with C1K were in the range of 3,828~4,220 ppm. These results suggested that mixtures of C1K and Comps or CTB combined with C1K triggered as elicitors for the enhancement of suppressive activity to the phytopathogens.
Nguyen, Dang-Minh-Chanh,Seo, Dong-Jun,Park, Ro-Dong,Lee, Bok-Rye,Jung, Woo-Jin The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Cucumber plants were infected with the root-knot nematode Meloidogyne incognita and were then treated with Cinnamomum cassia crude extracts (CCE). The activities of antioxidative enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), and ascorbate peroxidase (APX, EC 1.11.1.11, class I peroxidase) were detected in the cucumber leaves. On day 28 after treatment of C. cassia (0, 1, 5, and 10 mg/mL), the relative galling formation decreased significantly in a dose-dependent manner. The C. cassia crude extracts also effectively inhibited the growth of M. incognita in soil and roots. The number of second-stage juveniles was significantly decreased as the concentration of the C. cassia crude extracts increased. Twenty-eight days after treatment with CCE, the SOD activities of plant leaves treated with 5 and 10 mg/mL CCE were greater than that of leaves treated with nematode (Ne) only. In addition, the CAT and ascorbate peroxidase (APX) activities increased as the concentration of CCE increased. The relative activities of the antioxidative enzymes decreased as the galling formation in the roots increased in response to M. incognita infection. Polyacrylamide gel electrophoresis (PAGE) with activity staining revealed active bands of SOD corresponding to at least five isozymes (93, 79, 71, 66, and 41 kDa), including three Fe-SOD bands (71, 66, and 41 kDa) and two Mn-SOD bands (93 and 79 kDa). Analysis of the cucumber leaves revealed one active band corresponding to the CAT isozyme (>97 kDa) on 10% native gels. At least four bands corresponding to APX were observed in 10% polyacrylamide, including one major band (>97 kDa) and three minor bands (79, 71, and 66 kDa). Compared with the leaves of cucumber plants that received other treatments, SOD (41 kDa) and APX (70 kDa) isozymes in the leaves of plants treated with Ne only showed intensified bands in PAGE gels.
Dang-Minh-Chanh Nguyen,서동준,Ro-Dong Park,이복례,Woo-Jin Jung 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Cucumber plants were infected with the root-knot nematode Meloidogyne incognita and were then treated with Cinnamomum cassia crude extracts (CCE). The activities of antioxidative enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), and ascorbate peroxidase (APX, EC 1.11.1.11, class I peroxidase) were detected in the cucumber leaves. On day 28 after treatment of C. cassia (0, 1, 5, and 10 mg/mL), the relative galling formation decreased significantly in a dose-dependent manner. The C. cassia crude extracts also effectively inhibited the growth of M.incognita in soil and roots. The number of second-stage juveniles was significantly decreased as the concentration of the C. cassia crude extracts increased. Twenty-eight days after treatment with CCE, the SOD activities of plant leaves treated with 5 and 10 mg/mL CCE were greater than that of leaves treated with nematode (Ne) only. In addition, the CAT and ascorbate peroxidase (APX) activities increased as the concentration of CCE increased. The relative activities of the antioxidative enzymes decreased as the galling formation in the roots increased in response to M. incognita infection. Polyacrylamide gel electrophoresis (PAGE) with activity staining revealed active bands of SOD corresponding to at least five isozymes (93, 79, 71, 66, and 41 kDa), including three Fe-SOD bands (71, 66, and 41 kDa) and two Mn-SOD bands (93 and 79 kDa). Analysis of the cucumber leaves revealed one active band corresponding to the CAT isozyme (>97 kDa) on 10% native gels. At least four bands corresponding to APX were observed in 10% polyacrylamide, including one major band (>97 kDa) and three minor bands (79, 71, and 66 kDa). Compared with the leaves of cucumber plants that received other treatments, SOD (41 kDa) and APX (70 kDa) isozymes in the leaves of plants treated with Ne only showed intensified bands in PAGE gels.
Dang-Minh-Chanh Nguyen,Thi-Hoan Luong,Tien-Chung Nghiem,Woo-Jin Jung 한국응용생명화학회 2023 Journal of Applied Biological Chemistry (J. Appl. Vol.66 No.-
The aim of the present study was to evaluate the chemical composition and antioxidative activity of rhizome essential oil of Kaempferia parviflora Wall. ex Baker. The essential oil extracted by hydrodistillation was chemically profiled by GC/MS analysis. The antioxidative activity was determined and evaluated spectroscopically by the 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays. According to the results, the major essential oil components were camphene (18.03%), β- pinene (14.25%), a-pinene (12.38%), endo-borneol (10.23%), β- copaene (8.38%), and linalool (8.20%). K. parviflora rhizome oil possessed antioxidant potential, exhibiting DPPH and ABTS radical scavenging activities as high as 80.90 and 94.04%, respectively, at a concentration of 10 mg/mL. The corresponding IC50 values were 0.451±0.051 and 0.527±0.022mg/mL, respectively (IC50 values for ascorbic acid, as the standard, were 0.209±0.016 and 0.245±0.022 mg/mL, respectively). The mycelium of F. oxysporum was distorted and collapsed when treated with 0.5 mg/ mL of the EO of K. parviflora rhizome for 3 days treatment, which may provide an important information for exploring the metabolism of the fungicide K. parviflora rhizome and its derived compounds against F. oxysporum. This study provides the chemical properties of the essential oil of K. parviflora rhizome grown in Vietnam and their potential antioxidant and antifungal activities.
Dang-Minh-Chanh Nguyen,Thi-Hoan Luong,Van-Viet Nguyen,Woo-Jin Jung 한국응용생명화학회 2023 Journal of Applied Biological Chemistry (J. Appl. Vol.66 No.-
This study evaluated the in vitro nematicidal activity of Glycyrrhiza uralensis root extracts (GuRE) on Meloidogyne incognita eggs and juveniles. The results showed that treatment of M. incognita eggs with 2.0 mg/mL GuRE for 5 and 10 d resulted in 64.0 and 68.1% hatch inhibition, respectively. Furthermore, the relative mortality of J2 was 96.2% after treatment with 2.0 mg/mL GuRE for 48 h. Changes in the shape of the eggs and juveniles were determined after incubation with 2.0 mg/mL GuRE for 5 d and 48 h, respectively. These preliminary results suggest that GuRE can be used as an environment-friendly bio-nematicide to control root-knot nematodes. In the future, in vivo assays should be conducted using GuRE to ascertain its potential for widespread application as a nematicide.
( Dang-minh-chanh Nguyen ),( Thi-hoan Luong ),( Xuan-hoa Nguyen ),( Woo-jin Jung ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.2
In this study, we aimed to evaluate the nematicidal activity of cinnamon bark extracts (CE) and chitosan (Cs) against Meloidogyne incognita under in vitro, pot, and field conditions. In the pot experiments, CE mixed with Cs effectively inhibited M. incognita infection on black pepper plants. We applied the following formulations, which all significantly reduced the gall index and nematode number in 5 g of root and 100 g of soil: CE:Cs = 5 mg:20 mg, 10 mg:40 mg, and 15 mg:60 mg per pot. In addition, the application of CE:Cs = 32 mg:120 mg, 64 mg:240 mg, and 96 mg:360 mg per plant effectively reduced root gall formation and nematode density in black pepper roots and soil compared with that of the non-treated control under field conditions. Nematode density in the black pepper roots was positively correlated with the percentage of plants with yellow leaves. These results suggest that cinnamon mixed with chitosan may be used as an effective and eco-friendly pesticide against plant parasitic nematodes.
( Dang Minh Chanh Nguyen ),( Dong Jun Seo ),( Ro Dong Park ),( Bok Rye Lee ),( Woo Jin Jung ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Cucumber plants were infected with the root-knot nematode Meloidogyne incognita and were then treated with Cinnamomum cassia crude extracts (CCE). The activities of antioxidative enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), and ascorbate peroxidase (APX, EC 1.11.1.11, class I peroxidase) were detected in the cucumber leaves. On day 28 after treatment of C. cassia (0, 1, 5, and 10 mg/mL), the relative galling formation decreased significantly in a dose-dependent manner. The C. cassia crude extracts also effectively inhibited the growth of M.incognita in soil and roots. The number of second-stage juveniles was significantly decreased as the concentration of the C. cassia crude extracts increased. Twenty-eight days after treatment with CCE, the SOD activities of plant leaves treated with 5 and 10 mg/mL CCE were greater than that of leaves treated with nematode (Ne) only. In addition, the CAT and ascorbate peroxidase (APX) activities increased as the concentration of CCE increased. The relative activities of the antioxidative enzymes decreased as the galling formation in the roots increased in response to M. incognita infection. Polyacrylamide gel electrophoresis (PAGE) with activity staining revealed active bands of SOD corresponding to at least five isozymes (93, 79, 71, 66, and 41 kDa), including three Fe-SOD bands (71, 66, and 41 kDa) and two Mn-SOD bands (93 and 79 kDa). Analysis of the cucumber leaves revealed one active band corresponding to the CAT isozyme (>97 kDa) on 10% native gels. At least four bands corresponding to APX were observed in 10% polyacrylamide, including one major band (>97 kDa) and three minor bands (79, 71, and 66 kDa). Compared with the leaves of cucumber plants that received other treatments, SOD (41 kDa) and APX (70 kDa) isozymes in the leaves of plants treated with Ne only showed intensified bands in PAGE gels.