http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
李鍾文,신영진,李達雨 全北大學校 1979 論文集 Vol.21 No.-
The fading behaviour of sorghum pigment may be expressed by the equation F(H_NBS)―~4.00(1-e^-0.2055^t) F(V_NBS)―~24.00(1-e^-0.2055^t) F(C_NBS)―~19.00(1-e^-0.2055^t) F(ΔE)―~30.87 (1-e^-0.2055^t) ―~√(F(H_NBS)^2+F(V_NBS)^2+F(C_NBS)^2) where t is the fading time(hr), and F(H_NBS), F(V_NBS), F(C_NBS), and F(ΔE) are the changes of color difference of Hue, Value, Chroma and ΔE due to light fading.
Streptomyces thermocyaneoviolaceus의 Xylanase 생산조건 및 Xylooligo당의 생산
주길재,박희동,최준호,이인구,이오석,권달호 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-
농산폐자원으로부터 기능성물질인 xylooligo당을 생산하기 위해서 내열성 균주인 S.thermocyaneoviolaceus가 생산하는 xylanase의 생산최적조건을 검토한 결과 0.8% 밀기울, 0.06% yeast extract, 0.06% bactopeptone, 0.05% MgSO₄·7H₂O, 0.005% FeSO₄·7H₂O, 0.05% KH₂PO₄및 0.2% K₂HPO₄를 함유한 배지(pH7.0)에서 50℃, 24시간 배양시 최고효소활성(2.47 unit/ml)의 배양상징액을 얻을 수 있었다. 효소의 최적반응조건은 pH5.5, 65℃였다. 또한 pH안정성을 조사한 결과 pH4.5∼9.5사이에서 4℃에서 12시간후에도 80% 이상의 효소활성을 유지하였고, 열 안정성은 60℃에서 1시간 처리후 94%이상의 효소활성을 유지하는 내열성이 있는 효소였다. 생산된 xylanase birchwood xylan 반응생성물을 TLC 및 HPLC로 확인해 본 결과, pH가 낮을수록(pH 5.0∼6.0) xylobiose와 xylotriose및 소량의 xylose의 양이 증가하였고, pH가 높을수록 (pH8.0∼9.0) X₄이상의 각종 xylooligo당의 양이 상대적으로 증가하였다. 또한 24 시간후에는 xylan의 상대량이 25% 이하로 감소하면서 주분해산물로 xylobiose가 생산되었으며 xylotriose와 xylose 및 X₄이상의 각종 xylooligo당이 생산되었다. A thermotolerant bacterium, Streptomyces thermocyaneoviolaceus which produced xylan-degrading enzymes, utilized excellently xylan of wheat bran by producing the enzymes in comparison with that of birchwood or oat spelts. Optimal enzyme production was achieved in WB medium containing 0.8% wheat bran, 0.06% yeast extract, 0.06% bactopeptone, 0.05% MgSO₄·7H₂O, 0.05% FeSO₄·7H₂O, 0.05% KH₂PO₄ and, 0.2% K₂HPO₄(pH 7.0) at 50℃ for 24 hrs. The optimal pH and temperature for the hydrolysis of xylan were pH 5.5 and 65℃, respectively. The enzyme activity was retained more than 80% at the range from pH 4.5 to pH 9.5 at 4℃ for 12 hrs and 94% on the heat-treatment at 65℃ for 1 hr. Xylobiose, xylotriose, xylose, and other xylooligosaccharides were produced as end products from hydrolysis of birchwood xylan by the xylanase of S. thermocyaneoviolceus.
Dal Rhee, S.,Kim, C.H.,Seon Park, J.,Hoon Jung, W.,Bum Park, S.,Youn Kim, H.,Hwan Bae, G.,Jan Kim, T.,Young Kim, K. North-Holland ; Elsevier Science Ltd 2012 european journal of pharmacology Vol.691 No.1
Carbenoxolone is the 3-hemisuccinate of glycyrrhetinic acid, the active principal of licorice (Glycyrrhiza glabra). It was reported that carbenoxolone improved glucose tolerance with increased insulin sensitivity in mice with high fat diet-induced obesity. In the present study, we elucidated the protective effect of carbenoxolone in fatty liver animal models of C57BL/6-Lep<SUP>ob/ob</SUP> mice through inhibition of hepatic lipogenesis and apoptosis. In addition, the potential mechanisms by which carbenoxolone could exert such protection were elucidated. Carbenoxolone was daily administrated by gavage for 28 days in C57BL/6 and C57BL/6-Lep<SUP>ob/ob</SUP> mice. Carbenoxolone prevented the plasma triglyceride and free fatty acid accumulation associated with the reduction of the expression of sterol regulatory element binding protein-1c, liver X receptor, fatty acid synthase and acethyl-CoA carboxylase in the livers of C57BL/6-Lep<SUP>ob/ob</SUP> mice. Carbenoxolone also prevented hepatic injury through anti-apoptotic action in the livers of C57BL/6-Lep<SUP>ob/ob</SUP> mice, accompanied by increased Bcl-2 expression and suppressed Bax and cytochrome c expression. As a mechanism, increased inflammatory cytokine expressions were inhibited by carbenoxolone in the fatty livers of C57BL/6-Lep<SUP>ob/ob</SUP> mice. Furthermore, carbenoxolone inhibited free fatty acid (oleate/palmitate) induced reactive oxygen species formation and reversed free fatty acid induced mitochondrial membrane depolarization in HepG2 cells. Carbenoxolone prevents the development of fatty liver by inhibiting sterol regulatory element binding protein-1c expression and activity with an anti-apoptotic mechanism via the inhibition of inflammatory cytokine and reactive oxygen species formation in the livers of C57BL/6-Lep<SUP>ob/ob</SUP> mice. It is suggested that carbenoxolone prevents the development and progression of fatty liver disease in patients with insulin resistance.
Leptin inhibits rosiglitazone-induced adipogenesis in murine primary adipocytes
Rhee, Sang Dal,Sung, Yoon-Young,Jung, Won Hoon,Cheon, Hyae Gyeong Elsevier 2008 Molecular and cellular endocrinology Vol.294 No.1
<P><B>Abstract</B></P><P>Leptin mainly acts on the hypothalamus in the brain, in which it regulates food intake and energy expenditure. However, the direct effects of leptin on adipocytes have been controversial in the cellular level. In this study, the effects of leptin on rosiglitazone-induced adipocyte differentiation were investigated in the primary preadipocytes prepared from subcutaneous fat tissues of C57BL/6-<I>Lep</I><SUP><I>ob/ob</I></SUP> mouse. We found that acute and prolonged treatment of leptin on preadipocytes inhibited the rosiglitazone-induced transcription factor expression and adipocyte differentiation, respectively, accompanied with decreased expression of PPARγ and aP2. Either PD98059, an ERK inhibitor or fludarabine, a STAT1 inhibitor restored leptin-inhibited PPARγ expression and subsequent lipid accumulation, but inhibitors for PI-3K (LY294002) and for STAT3 (piceatannol) did not. Furthermore, leptin decreased PPARγ expression also in fully differentiated adipocytes, which was reversed by either PD98059 or fludarabine. Taken together, these data suggest that leptin has a direct inhibitory effect on the rosiglitazone-induced adipocyte differentiation and PPARγ expression, in which ERK1/2 MAP kinase and JAK/STAT1 signaling pathways are involved.</P>
Sang Dal Rhee,Hee-Youn Kim,Won Hoon Jung,Ji Young Sohn,Min Soo Kho,Hyae Gyeong Cheon 한국실험동물학회 2009 Laboratory Animal Research Vol.25 No.4
The previously reported methods for genotyping Lep<SUP>ob</SUP> mice have shortcoming of either inconveniency or poor reproducibility. Therefore we improved up these protocols by adding the mismatched base pairs in the allele-specific primers for stable discrimination of single base pair mismatch and amplifying the polymerase chain reaction (PCR) product in one tube. This new simplified genotyping method results in higher reproducibility even with crudely prepared DNA. When the method was used to select heterozygotic breeders to produce obese mice, 256 pairs of the all 287 mating pairs produced obese offspring, providing 90% or greater genotyping efficiency. This method using PCR with mismatched allele specific primers is appropriate and efficient for genotyping a large number of Lep<SUP>ob</SUP> mutants.
양자화학적 계산에 의한 살리씰산유도체의 정량적 구조-활성 상관관계
이종달(Jong Dal Rhee) 대한약학회 1988 약학회지 Vol.32 No.1
QSAR of Salicylic acid derivatives, as anti-inflammatory agent, classified into Group I (not-having-5-phenyl ones) and Group II (having-5-phenyl ones) were investigated by quantum chemical calculations. The results are below: not significant statistically for both of Group I and Group II, but significant for each Group. potency=-8.46X5(+/-4.05) + 1.639(+/-0.5) n=5 r=0.77 se=0.31 for Group I. Where X5 means charge of carbon atom bonded to hydroxyl radical. potency=-O.16X19(+/-0.17) + 7427.38HO(+/-10.18) - 6629.85X15(+/-11.70) + 4977.40X10(+/-33.78) + 351.51X5(+/-4.41) + 3378.84(+/-13.13) n=7 r=O.99 se=0.019 for Group II. where X19 and X15 stand for charges of the para carbon and the first carbon atoms in phenyl radical, respectively and X10, charge of carboxylic carbon atom, HO, HOMO energy. It seems to be possible to qualitatively predict potency of drug by Pearson''s HSAB theory. It means that drug should possess low LUMO energy and high HOMO energy.