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권달호,류병호,김희숙,김경순,이세윤,박종옥 ( Dhal Ho Kwon,Byung Ho Rheu,Hee Sook Kim,Kyung Soon Kim,Seh Yoon Yi,Jong Ok Park ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6
Trichosanthis Radix (TR) has been used in traditional herbdrugs as an antitumor and immunologic stimulating agent. Lectin was purified to homogeniety from Trichosanthis Radix by ammonium sulfate, DEAF-cellulose chromatography and Sephadex G-150 gel filtration. Trichosanthis Radix lectin (TRL) had agglutination activity of human erythrocytes, and thrombocytes. In addition, Escherichia coli and Pseudomonas aeruginosa, Gram negative, were shown agglutination, but Proteus, Hemophilus influenzae and Staphylococcus aureus were not agglutinated by TRL. Following injection of TRL into ICR mouse, γ-globulin was increased to 83% and the number of lymphocytes was increased to 18%. These results suggest that TRL may have stimulating effects on general immune systems.
권달호,류병호,김희숙,김경순,이세윤,박종옥,Kwon, Dhal-Ho,Rheu, Byung-Ho,Kim, Hee-Sook,Kim, Kyung-Soon,Yi, Seh-Yoon,Park, Jong-Ok 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6
한방재료 중에서 지혈, 창종, 부종의 치료에 사용되는 천연식물 70여종을 택하여 적혈구응집소의 존재 유무를 검색하였다. 그 중 괄루근(Trichosanthis Radix, TR)으로부터 렉틴을 분리정제하였다(TRL). 황산암모늄 침전법, DEAE-cellulose ion-exchange chromatography, Sephadex G-150 gel filtration 방법 등에 의해 72.95배 정제하였고 회수율은 9.96%이였다. 정제된 렉틴은 glycoprotein으로 polyacrylamide gel electrophoresis에서 단일의 띠를 보여주어 거의 순수하게 정제됨을 나타내었다. TRL은 적혈구, 임파구, 혈소판에 대해 응집현상을 나타내었고 그 외 박테리아 응집효과에서 Escherichia coli와 Pseudomonas aeruginosa를 응집하였다. 또한 TRL을 ICR mouse에 투여했을 때 백혈구와 임파구의 증가를 가져와 면역기구중의 effector로서 면역감응세포의 증식 분화를 유발시키는 것으로 추측되었다. Trichosanthis Radix (TR) has been used in traditional herbdrugs as an antitumor and immunologic stimulating agent. Lectin was purified to homogeniety from Trichosanthis Radix by ammonium sulfate, DEAF-cellulose chromatography and Sephadex G-150 gel filtration. Trichosanthis Radix lectin (TRL) had agglutination activity of human erythrocytes, and thrombocytes. In addition, Escherichia coli and Pseudomonas aeruginosa, Gram negative, were shown agglutination, but Proteus, Hemophilus influenzae and Staphylococcus aureus were not agglutinated by TRL. Following injection of TRL into ICR mouse, ${\gamma}-globulin$ was increased to 83% and the number of lymphocytes was increased to 18%. These results suggest that TRL may have stimulating effects on general immune systems.
Streptomyces thermocyaneoviolaceus의 Xylanase 생산조건 및 Xylooligo당의 생산
주길재,박희동,최준호,이인구,이오석,권달호 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-
농산폐자원으로부터 기능성물질인 xylooligo당을 생산하기 위해서 내열성 균주인 S.thermocyaneoviolaceus가 생산하는 xylanase의 생산최적조건을 검토한 결과 0.8% 밀기울, 0.06% yeast extract, 0.06% bactopeptone, 0.05% MgSO₄·7H₂O, 0.005% FeSO₄·7H₂O, 0.05% KH₂PO₄및 0.2% K₂HPO₄를 함유한 배지(pH7.0)에서 50℃, 24시간 배양시 최고효소활성(2.47 unit/ml)의 배양상징액을 얻을 수 있었다. 효소의 최적반응조건은 pH5.5, 65℃였다. 또한 pH안정성을 조사한 결과 pH4.5∼9.5사이에서 4℃에서 12시간후에도 80% 이상의 효소활성을 유지하였고, 열 안정성은 60℃에서 1시간 처리후 94%이상의 효소활성을 유지하는 내열성이 있는 효소였다. 생산된 xylanase birchwood xylan 반응생성물을 TLC 및 HPLC로 확인해 본 결과, pH가 낮을수록(pH 5.0∼6.0) xylobiose와 xylotriose및 소량의 xylose의 양이 증가하였고, pH가 높을수록 (pH8.0∼9.0) X₄이상의 각종 xylooligo당의 양이 상대적으로 증가하였다. 또한 24 시간후에는 xylan의 상대량이 25% 이하로 감소하면서 주분해산물로 xylobiose가 생산되었으며 xylotriose와 xylose 및 X₄이상의 각종 xylooligo당이 생산되었다. A thermotolerant bacterium, Streptomyces thermocyaneoviolaceus which produced xylan-degrading enzymes, utilized excellently xylan of wheat bran by producing the enzymes in comparison with that of birchwood or oat spelts. Optimal enzyme production was achieved in WB medium containing 0.8% wheat bran, 0.06% yeast extract, 0.06% bactopeptone, 0.05% MgSO₄·7H₂O, 0.05% FeSO₄·7H₂O, 0.05% KH₂PO₄ and, 0.2% K₂HPO₄(pH 7.0) at 50℃ for 24 hrs. The optimal pH and temperature for the hydrolysis of xylan were pH 5.5 and 65℃, respectively. The enzyme activity was retained more than 80% at the range from pH 4.5 to pH 9.5 at 4℃ for 12 hrs and 94% on the heat-treatment at 65℃ for 1 hr. Xylobiose, xylotriose, xylose, and other xylooligosaccharides were produced as end products from hydrolysis of birchwood xylan by the xylanase of S. thermocyaneoviolceus.
박종옥,권달호,김희숙,류병호,이세윤,김경순 慶星大學校 1993 論文集 Vol.14 No.2
Since ancient times Trichosanthis Radix has been used in traditional herbdrung as an antitumor and immunologic stimulating agent. Lectin was purified to homogeneity from Trichsanthis Radix by ammonium sulfate precipitation, DEAE-Cellulose chromatography and Sephadex G-150 gel filltration. The major amino acid was tyrosine and some aspartic acid, glutamic acid and saccharides were also present. Trichosanthis Radix Lectin (TRL)strongly stimulated activity of human erythrocytes, lymphocytes, and thrombocytes. In addtion, agglutination of gram negative Escherichia coli and Pseudomonas ararugimosa was shown, but proteus, Hemophilus influenza and Staphylococcus aureus were not agglutinated. Folloaing injection of TRL into ICR mouse, granulocyte was increased by 87.5% and the number of lymphocyte was increased by 25-28%. These results suggest that TRL may have stimulating effects on the general immune systems.