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Kim, Min Keun,Kang, Tae Ho,Kim, Sung Kyum,Jeong, Yu Seok,Yun, Han Dae,Kim, Hoon The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.6
The rsmA gene was cloned from soft-rot bacterium Pectobacterium carotovorum subsp. carotovorum LY34 (Pcc LY34), and its role in pathogenicity was investigated by marker exchange mutagenesis. From a cosmid library of Pcc LY34 genomic DNA, a positive clone carrying the rsmA gene was selected, and the gene was cloned by polymerase chain reaction (PCR) amplification. The gene is 186 bp in size and encodes a protein of 62 amino acids with a predicted molecular mass of 6,839 Da. The calculated pI of the RsmA is 8.16. The phylogenetic tree showed that the RsmA of Pcc LY34 appeared genetically identical to the CsrA of Pectobacterium atrosepticum SCRI1043 (100% identity) and similar to the CsrA of Yersinia pestis KIM10+(98.3%). The gene was disrupted by the $Km^r$ gene, and the cells became mutated (i.e., $RsmA^-$ mutant). The pathogenicity test revealed that the disease rating of the $RsmA^-$ mutant only differed slightly from that of the wild type on a slice of potato tuber and a Chinese cabbage stalk. These results suggest that RsmA is not an essential factor for the pathogenicity of Pcc LY34 and that the rsmA gene of Pcc LY34 is not completely derepressed in the $RsmA^-$ mutant for virulence-related genes, contrary to the results of Erwinia carotovora subsp. carotovora $RsmA^-$ mutant, which proved hypervirulent for celery petioles. These results showed that the microenvironmental conditions of the host and/or strain of pathogen are important for the coordination of virulence gene expression.
Analysis of Japanese Demand for Alaska Groundfish
Kim, Dae Kyum 한국수산경영학회 1985 수산경영론집 Vol.16 No.2
In 1977, the United States enacted the Magnuson Fishery Conservation and Management Act (MFCMA), which established U.S. Fisheries Conservation Zone (FCZ). The MFCMA grants preference to U.S. harvesters over foreign fleets in the U.S. FCZ. At present, the large stocks of groundfish in the U.S. FCZ off the Alaska coast have been under-utilized in the U.S. domestic market and the fisheries for these groundfish are dominated by foreign fleets. Hence, expected benefits from replacing foreign fisheries by domestic fleets will accrue to the U.S. fishery only by exporting the increased U.S. products to foreign countries. U.S. exports may be dependent on the price levels in the foreign markets raised by the reduced foreign catch from U.S. waters. In this paper, Japanese demand models for Alaska groundfish were estimated. The derived coefficient from the estimated models suggest that a decrease in the Japanese landings from the U.S. FCZ by a thousand metric tons will increase pollock price by 0.017 Yen/㎏, cod price by 0.351 Yen/㎏, flatfish by 1.074 Yen/㎏, and ocean perch by 1.347 Yen/㎏ in the Japanese market. These results based on percentage would increase 19 percent for pollock price, 11 percent for cod price, 40 percent for flatfish, and 2 percent for ocean perch price.
A Large and Changing U . S . Market for Gadoids and Other Groundfish
Kim, Dae Kyum 한국수산경영학회 1986 수산경영론집 Vol.17 No.1
The United States is the world's leading importer of frozen processed groundfish products, with over two thirds of total world imports. Over 90% of the U.S. groundfish demand is met by imports, while about 2 million mt of groundfish are taken from U.S. waters by joint-venture and foreign fleets. The objective of this paper is to provide descriptive information concerning the U.S. groundfish market and the potential for groundfish resources off Alaska to become a major source of supply to this market. The size of the U.S. market, U.S. imports, trade policies, and catch from U.S. waters are discussed, and a comparison is made between the potential domestic catch of groundfish off Alaska and current U.S. groundfish consumption. The total optimum yield of 2.3 million mt for flounders, cod, and pollock is about four times the round weight equivalent of U.S. imports of these species in 1984.
EVpedia: a community web portal for extracellular vesicles research.
Kim, Dae-Kyum,Lee, Jaewook,Kim, Sae Rom,Choi, Dong-Sic,Yoon, Yae Jin,Kim, Ji Hyun,Go, Gyeongyun,Nhung, Dinh,Hong, Kahye,Jang, Su Chul,Kim, Si-Hyun,Park, Kyong-Su,Kim, Oh Youn,Park, Hyun Taek,Seo, Ji H Oxford University Press 2015 Bioinformatics Vol.31 No.6
<P>Extracellular vesicles (EVs) are spherical bilayered proteolipids, harboring various bioactive molecules. Due to the complexity of the vesicular nomenclatures and components, online searches for EV-related publications and vesicular components are currently challenging.</P>
다중지지된 배관계통의 내진해석에 적용된 MIRS 방법의 Parametric Study
김대수(DAE SOO KIM),김수겸(SOO KYUM KIM),원윤호(YOUN HO WON),이대건(DAE GEON LEE),박문백(MUN BACK PARK) 한국소음진동공학회 2015 한국소음진동공학회 학술대회논문집 Vol.2015 No.4
The steam supply piping connected to the high pressure(HP) turbine of APR1400 (Korea’s advanced power plant 1400 MW-class) is a typical example of multi-supported piping system, and it is routed from the Containment building to the Turbine building via the Main Steam Isolation Valve House in the Auxiliary building. In the seismic analysis of this piping system, using the Enveloped Response Spectrum (ERS) method, a commonly used methodology for seismic analysis of piping systems in the nuclear industry, generates overly conservative analysis results. Therefore, Time History Method (THM) which applies excitation characteristic of each support attached to individual building was used to eliminate unnecessary conservatism. However, it was noticed that the Time History Method requires considerable amount of labor and time in generating combined time history equivalent to the spectrum applied for each support although it is regarded as the most exact and realistic method for seismic analysis. The nuclear industry has been making lots of efforts in finding out the mathematic logicality and practical applicability to resolve this issue. This paper deals with parametric research on combination effects of responses between support groups, damping effects, and modal combination method with close modes in applying the Multiple Input Response Spectrum (Independent Support Motion: ISM) method to the analysis model of the steam supply piping connected to the high pressure turbine of APR1400. Quantitative assessment and comparison with the analysis results of the ERS method and THM were also carried out. As a result, it is shown that the analysis results of the MIRS method together with the SRSS combination between support groups, 4% damping with ±15% spectrum peak broadening and grouping of modal combination are remarkably similar to those of THM.