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Bearing Capacity Test and Calculation Theory on CHS X-Joints Stiffened with Joint-Plates
Chenzhong Gao,Dachang Zhang,Mingcheng Cui,Peng Peng 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2
In this study, three circular hollow sections (CHS) tubular X-joints strengthened with different external stiffeners or not were investigated by experimental and nonlinear finite element anlaysis (FEA) to study the bearing capacities, failure modes, and the strengthening effect of joint-plates. The test results showed that the bearing capacity of the X-joints could be improved by using the stiffened joint-plate, and the joint failure process was delayed, and the cambered surface of chord was straightened by tensile braces. Compared with the tested results, the nonlinear FEA could simulate the failure modes and its bearing capacity behavior of steel tubular X-joints well. To estimate the contribution of joint-plates on bearing capacity, four kinds of parameters were selected, and the corresponding FEA of stiffened X-joint were performed. Then, the influencing law on bearing capacity of different parameters was investigated. According to the superposition method of bearing capacities, the annular calculation model and the multi-parameter calculation theory of stiffened circular steel tubular X-joints were proposed, in which the coefficients were determined through regression analysis. Lastly, the comparisons on tested, simulated, and theoretical bearing capacities of stiffened X-joints were carried out, and there had good agreement between them.
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Load-transferring mechanism and evaluation theory of bolt with single and double nut fasteners
Qiyu Li,Dachang Zhang,Hao Xu,Yibi Li,Weiqun Chen,Kaixuan Zhang 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.86 No.2
The use of the ordinary double nut (i.e., ODN) composed of a master nut (i.e., M-nut) and a slave nut (i.e., S-nut) is a highly efficient method to prevent bolts loosening. A novel double nut (i.e., FODN) composed of a master nut (i.e., M-nut) and flat slave nut (i.e., FS-nut) is proposed to save raw materials. The bolt fastening tests with single nut, ODN and FODN are performed to investigate the preload and counterbalance forces. Corresponding finite element analysis (FEA) models are established and validated by comparing the preload with the experimental results. The load-bearing capacity, the extrusion effect, and the contact stress of each engaged thread for ODN and FODN are observed by FEA. The experimental and simulated results revealed that the bolt fastening with double-nut has different load-transferring mechanisms from single-nut. Nevertheless, for double-nut/bolt assemblies, the FS-nut can provide load transfer that is like that of the S-nut, and the FODN is a reasonable and reliable fastening method. Furthermore, based on the theory of Yamamoto, a formula considering the extrusion effect is proposed to calculate the preload distribution of the double-nut, which is applicable to varying thicknesses of slave-nuts in double-nut/bolt assemblies.
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Yunqiang Liu,Sizhong Zhang,Dachang Tao,Yuan Yang,Yongxin Ma 한국유전학회 2014 Genes & Genomics Vol.36 No.2
The testis-enriched genes ZNF230/Znf230 arelocated on human chromosome 11p15/mouse chromosome7 near conserved imprinting control regions. Typical CpGislands (CGIs) extend from the promoter to the first exon ineach of these genes. To investigate the correlation betweenthe methylation status of the above CGIs and the expressionpatterns of the two genes, we performed bisulfitegenomic sequencing of genomic DNA from human andmouse tissues and cells. The results showed that the CGIsof ZNF230/Znf230 were completely unmethylated in allselected tissues and cells, regardless of the expressionlevels of the two genes. Further experiments using Znf230-second-exon-knockout mice to investigate the imprintingstatus of Znf230 showed that its expression was notaffected by genomic imprinting. However, an in vitromethylation assay illustrated that the methylation of theseCpG sites could repress the expression of the luciferasereporter gene. Furthermore, chromatin immunoprecipitationwith anti-Specificity protein 1 (Sp1) antibody showedthat Sp1 could bind to the CGIs in the ZNF230/Znf230gene promoter. Thus, we propose that the unmethylatedstate of ZNF230/Znf230 CGIs may be a prerequisite fortheir expression but not sufficient for their abundantexpression in the testis, and that Sp1 binding may be onefactor involved in preserving the methylation-free state ofZNF230/Znf230 CGIs.
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Li, Na,Sun, Huaqin,Wu, Qiaqing,Tao, Dachang,Zhang, Sizhong,Ma, Yongxin Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.2
We have cloned a novel mouse zinc finger protein gene Znf313 by rapid amplification of cDNA ends (RACE) according to the homologue of human ZNF313 gene. The cDNA is 2,163 base pairs (bp) in length and encodes a 229 amino acids (aa) protein with a $C_3HC_4$ ring finger domain and three $C_2H_2$ domains. 89% and 93% nucleotide (nt) and aa sequence identity is observed with its human homologue. Revealed by Northern blot and RT-PCR, full mRNA consists of 2.16 kb and widely expresses in tissues as a single transcript, most abundantly in heart, liver, kidney and testis. The expression of Znf313 in testis is detected in all development stages. Western blot analysis also reveals that Znf313 is expressed in the tissues. Immunohistochemical staining and subcellular localization demonstrate that Znf313 is expressed both in the cytoplasm and nucleus whereas predominantly localized in the nucleus. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation in organism and relates to spermatogenesis.
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