Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

제1유구치의 조기 상실로 인한 공간 변화에 대한 3차원적 분석

김지연,정다운,곽소연,유승은,박기태 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

본 연구는 제1유구치의 조기 상실 시 공간 변화에 대한 분석을 위하여 3-Dimensional Laser Scanner를 이용하여 그 유용성을 평가하고자 하는 예비논문이다. 6세-8세 사이의 제1대구치가 맹출하여 확실한 교합 관계를 이루는 혼합치 열기 어린이 중 편측 제1유구치의 조기 발치가 필요한 6명의 발치 전 후의 모형을 이용하여 다음과 같은 결과를 얻었다. l. 유구치 공간변화는 상악은 실험군과 대조군 모두 현저한 감소를 보이지 않았고, 하악은 실험군에서 3명 중 2명에서 공간감소가 관찰되었다. 2. 치열궁 너비,치열궁 둘레는 초기모형과 최종모형에서 비슷한 값을 보였다. 3. 협설측 경사도 변화는 유견치에서는 상하악 모두 특별한 경향을 보이지 않았으며,제2유구치는 상악은 실험군과 대조군이 유사하였고,하악에서는 3명중 2명에서 실험군이 더 큰 설측경사도 변화를 보였다. 제1대구치는 하악에서 실험군이 더 큰 설측경사도 변화가 관찰되었다. 4. 근원심 경사도 변화 측정에서 유견치는 상하악 모두 특별한 경향이 관찰되지 않았고 제2유구치의 경우 하악에서 실험군이 더 큰 근심경사도 증가가 보였다. 제1대구치는 상악에서 실험군,대조군 모두 원심 경사도가 증가되었다. The purpose of this study was to evaluate a 3-Dimensional laser scanner for the space analysis after loss of a primary first molar. Six children with premature loss of a primary first molar were examined using study models taken before and after the extraction. The results were as follows: 1. There was no change in primary molar space after the extraction of a maxillary primary first molar However, 2 out of 3 children experienced primary molar space loss in extraction side of a mandibular primary first molar. 2. Arch width and arch perimeter showed no difference between initial and final model. 3. All primary canines did not show any changes in inclination. Maxillary primary second molars had similar changes in both extraction and control side. However, 2 out of 3 mandibular primary second molars in extraction side showed more lingual tipping compared to control side. Mandibular permanent first molars tipped more lingually in extraction side. 4. In angulation, primary canines showed nothing of significance. Mandibular primary second molars tipped more mesially in extraction side than in control side. Maxillary permanent first molars have increased distal angulation after extraction of primary first molars in both side.

3차원 그래픽스를 이용한 실시간 로봇 모니터링 및 예측

김진구,정다운,전재욱 成均館大學校 科學技術硏究所 1997 論文集 Vol.48 No.1

In industrial automation lines, the main method to transfer information,, about robots is using some digital I/O in robot systems. Through these digital I/O, the state of each robot task is monitored whether it has been performed correctly or not. But this method can only communicate some informations among predefined states of each robot task and do no informations about other states during each robot task. In order to monitor detailed states of robot systems during each task, robot systems have used a video display or a graphics system. Some researches have used graphics systems to make real-time display and replay in addition to off-line simulation. This paper proposes a technique to monitor real time robot motions and to predict robot motions by using 3-d graphics. A graphics system is used to represent a virtual robot so that some c ordination between a real robot and this virtual robot can be achieved. With this coordination, monitoring and prediction of robot, motions will be performed.

디메틸포름아미드 노출 근로자의 요중 N-메틸포름아미드 분석시 GC-NPD와 GC-FID 방법간 비교

정다운,김현욱 가톨릭대학산업의학센타 산업의학연구소 1999 韓國의 産業醫學 Vol.38 No.1

This purpose of the this study was to compare the results of two analytical techniques, GC-NPD and GC-FID, of quantifying urinary NMF from 70 DMF exposed workers in synthetic resins, leather, and dye manufacturing industries. In addition, the relationship between conecntrations of airborne DMF and urinary NMF was investigated using samples collected from 12 workers. Airborne DMF was sampled using both charcoal and silicagel tubes. All urine specimens were collected both prior-to and at end-of workshifts. The results were as follows: 1. The detection limits of urinary NMF by GC-FID and GC-NPD were 0.043㎎/l and 0.009㎎/l, respectively. The recovery rates were 96.9% for the GC-NPD and 90.2% for the GC-FID methods. 2. Desorption efficiencies of DMF on Charcoal tubes were very poor with 32.96%, while those on silicagel tubes were marginally acceptable with 85.70%. 3. A statistically significant relationship between concentrations of airborne DMF and urinary NMF from 12 workers determined by GC-NPD (r=0.74) and by GC-FID (r=0.67) was observed. 4. Geometric mean concentrations of urinary NMF of the end-of-shift urines from 70 workers were 15.84㎎/g creatinine by GC-NPD and were 9.88㎎/g creatinine by GC-FID, respectivery. For the prior-to-shift samples, they were 6.05 ㎎/g creatinine by GC-NPD and 0.92㎎/g creatinine by GC-FID. These results of this study suggest that for collecting airborne DMF in the workplace, silicagel tubes should be utilized. For quantifying urinary NMF concentrations as a biological marker of DMF exposed workes, urine should be collected at the end-of-shift. Urinary NMF should be analyzed by GC-NPD because of its lower of detection and better precision than that of GC-FID.

시중에 판매되고 있는 아이스크림의 일반세균 및 대장균군 오염 실태

정다운,김영환,손종렬,변상훈 高麗大學校 倂設 保健大學 保健科學硏究所 2003 保健科學論集 Vol.29 No.1

Ice cream is a vastly consumed food enjoyed by a wide range of people. Therefore, it is easily accessible and amounts to hundreds of its kind. Ice cream is a ready-made food which could be served without undergoing cooking process and thus putting weight on its hygienic condition. And so we set out to conduct some research on the number of E-coli bactria contained in commercially sold ice creams. We applied Lactose broth method along with Desoxycholate agar method which enables us to verify the existence of E-coli bacteria and how much is contained. We focused our research on soft ice creams and aimed to seek out the degree to which they were contaminated by E-coli bacteria. 4 major distributors of soft ice cream out of 5 didn't meet the maximum allowance of E coli bacteria to be found. The number of general bacteria observed per 1 ㎖ of specimen must be held at less than 100,000, and this wasn't violated by any of the companies' samples. Yet on E-coli testing, all of K, L, B and M companies' samples respectively exceeded the permitted limits of bacteria to be detected.

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.

Characterization of Nivalenol-Producing Fusarium culmorum Isolates Obtained from the Air at a Rice Paddy Field in Korea

Kim, Da-Woon,Kim, Gi-Yong,Kim, Hee-Kyoung,Kim, Jueun,Jeon, Sun Jeong,Lee, Chul Won,Lee, Hyang Burm,Yun, Sung-Hwan The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.3

Together with the Fusarium graminearum species complex, F. culmorum is a major member of the causal agents of Fusarium head blight on cereals such as wheat, barley and corn. It causes significant yield and quality losses and results in the contamination of grain with mycotoxins that are harmful to humans and animals. In Korea, F. culmorum is listed as a quarantine fungal species since it has yet to be found in the country. In this paper, we report that two isolates (J1 and J2) of F. culmorum were collected from the air at a rice paddy field in Korea. Species identification was confirmed by phylogenetic analysis using multilocus sequence data derived from five genes encoding translation elongation factor, histone H3, phosphate permease, a reductase, and an ammonia ligase and by morphological comparison with reference strains. Both diagnostic PCR and chemical analysis confirmed that these F. culmorum isolates had the capacity to produce nivalenol, the trichothecene mycotoxin, in rice substrate. In addition, both isolates were pathogenic on wheat heads and corn stalks. This is the first report on the occurrence of F. culmorum in Korea.

감마선 조사가 미역귀 조다당의 추출수율 및 미백활성 변화에 미치는 영향

김다미(Da-Mi Kim),김경희(Kyoung-Hee Kim),성낙윤(Nak-Yun Sung),정필문(Pil-Moon Jung),김정수(Jeong-Soo Kim),김재경(Jae-Kyung Kim),김재훈(Jae-Hun Kim),최종일(Jong-il Choi),송범석(Beom-Seok Song),이주운(Ju-Woon Lee),김진규(Jin-Kyu Kim),육 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.5

본 연구에서는 해조류에 대한 방사선의 영향 평가에 관하여 알아보기 위하여 감마선 조사된 미역귀로부터 추출된 조다당의 추출수율 및 미백활성에 관하여 관찰하였다. 미백활성은 melanin 합성에 중요하게 작용하는 tyrosinase 억제 활성과 melanocyte의 melanin 생성 억제능을 평가하였다. 실험 결과, 방사선의 조사는 선량 의존적으로 조다당의 추출수율을 증가시키고, tyrosinase 억제 활성 및 α-MSH로 melanin을 과생성 시킨 B16BL6 melanoma 세포내 melanin 생성 억제 활성을 유지시켜 주는 것으로 나타났다. 이러한 결과로 미루어 보아 감마선의 조사는 미백활성을 갖는 미역귀 조다당을 얻기 위한 효과적인 방법이며, 미백 산업 분야에서도 효과적으로 이용될 수 있을 것으로 사료된다. We investigated changes in the extraction yield and whitening activity of polysaccharides from Undaria pinnatifida sporophyll due to gamma irradiation. U. pinnatifida sporophyll was gamma-irradiated at doses of 10, 30, 50, 70, and 100 kGy, then extracted with hot water and precipitated with ethanol to extract polysaccharides. Crude polysaccharide yields increased with an increase in irradiation dosage, but tyrosinase inhibition activity did not change. Melanin synthesis did not significantly differ between B16BL6 cells treated with irradiated and non-irradiated samples. In conclusion, gamma irradiation increased the crude polysaccharide extraction yield but did not change the whitening activity of U. pinnatifida sporophyll. This implies that gamma irradiation can be used to increase yields in the cosmetic industry, thus increasing profits.

Effects of Banhabaekchulcheonma-Tang on Neuronal Cell Protection and Cognitive Function Recovery through Inhibition of Microglia and Astrocyte Activation in BCAS Model Mice

( Kim Da-woon ) 대구한의대학교 제한동의학술원 2022 제한동의학술원논문집 Vol.20 No.1

PURPOSE : Vascular dementia (VD) is the second most prevalent type of dementia after Alzheimer’s disease, and no drug has been approved for treatment yet. The effect and mechanism of Banhabaekchulcheonma-Tang (BBCT) on ischemic brain injury and cognitive function impairment caused by bilateral carotid artery stenosis (BCAS) in mice were investigated. METHODS : Mice were diviaed into four groups of 5 each. Sham-operated and BCAS control groups were orally administered with equal volumes of vehicle. BBCT L group and BBCT H group were administered 40 ㎖/kg and 80 ㎖/kg of BBCT, respectively, but the volumes were adjusted to be the same. Vehicle and BBCT were administered three times a week for six weeks from two weeks after surgery. Body weight changes, blood pressure, and pulse rate were measured once every two weeks. Behavioral tests, which included the Y-maze test and Novel object recognition test (NORT), were performed eight weeks after surgery, and mice were sacrificed for immunofluorescence staining (IF staining) and RNA sequencing. STRING database was used for building and analyzing the protein interaction network. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses were conducted to identify the main targets and pathways of BBCT against VD. RESULTS : Compared to the sham-operated group, the body weight decreased significantly in the BCAS control group and the BBCT L, H groups. All groups had no significant differences in blood pressure, pulse rate, and serum electrolytes. There was no significant difference in cognitive function measured by the Y-maze test in all groups. Cognitive function was significantly improved in the BBCT H group compared to the BCAS control group in NORT. In IF staining of the brain cortex, the expression of CD68+ microglia and GFAP+ astrocytes was suppressed in the BBCT H group. The administration of BBCT H restored gene expression dysregulations caused by BCAS surgery. CDC20, EGF, and TRAF1 were predicted as major target proteins by the STRING database. According to KEGG analysis, BBCT was predicted to regulate the neuroactive ligand-receptor interaction pathway mainly. GO analysis showed that BBCT mainly regulates the neuropeptide signaling pathway in biological processes. CONCLUSION : BBCT significantly improved cognitive impairment in BCAS model mice with neuroprotective effects through inhibiting microglia and astrocyte activation, regulating the expression of CDC20, EGF, TRAF1, and key proteins in the neuroactive ligand-receptor interaction pathway and neuropeptide signaling pathway.

Causes and treatments of Diarrhea in laboratory cynomolgus monkey

Da-Hee Kim,Seong-Hyun Kim,Ji-Woo Jang,Ji Min Park,Ji-Woon Lee,Pill-Soo Lee,Bo-Kyung Kim,Doo-Wan Cho,Su-Cheol Han,Young-Su Yang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Diarrhea is a commonly observed health problem in laboratory monkeys and it was related to numerous factors such as infection (bacteria, viruses, and parasitic organism), nutritional, and/or psychologic factors. We herein report cases of diarrhea cause and treatment and the data were obtained from approximately 400 monkeys, aged 2-6 years, at our laboratory between 2020 and 2021. Diarrhea is most often observed in the case of changes the diet and rearing environment during quarantine. It is observed in about 10-20% of all animals, and this symptom may resolve naturally within 7 days. At this time, feeding probiotics such as lactic acid-producing bacteria or providing additional fruit helps to improve symptoms quickly. However, some individuals show signs of thinning and dehydration as these symptoms continue, and rectal prolapse was happen in severe cases. In this case, fluid therapy and surgical correction are necessary. Protozoan infection such as Balantidium and Giardia also occurs occasionally, and in this case, treatment with metronidazole and paromomycin was helpful. In addition, stress or unknown causes may occur, and in this case, methods such as group housing of 2-3 animals or symptomatic therapy (Lactobacillus feeding, diet control, behavior enrichment) can be considered. Maintaining the animal"s health is important for accurate interpretation of results and this article may lead to better management of diarrhea among monkeys in laboratory facilities.