Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

Chung, H.J.,Kim, K.W.,Han, D.W.,Lee, H.C.,Yang, B.C.,Chung, H.K.,Shim, M.R.,Choi, M.S.,Jo, E.B.,Jo, Y.M.,Oh, M.Y.,Jo, S.J.,Hong, S.K.,Park, J.K.,Chang, W.K. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

IL-32γ inhibits cancer cell growth through inactivation of NF-κB and STAT3 signals

Oh, J H,Cho, M-C,Kim, J-H,Lee, S Y,Kim, H J,Park, E S,Ban, J O,Kang, J-W,Lee, D-H,Shim, J-H,Han, S B,Moon, D C,Park, Y H,Yu, D-Y,Kim, J-M,Kim, S H,Yoon, D-Y,Hong, J T Nature Publishing Group 2011 Oncogene Vol.30 No.30

<P>Several studies have shown physiological functions of interleukin (IL)-32, a novel cytokine. However, the role of IL-32 in cancer development has not been reported. In this study, we showed that IL-32γ inhibited tumor growth in IL-32γ-overexpressing transgenic mice inoculated with melanoma as well as colon tumor growth in xenograft nude mice inoculated with IL-32γ-transfected colon cancer cells (SW620). The inhibitory effect of IL-32γ on tumor growth was associated with the inhibition of constitutive activated nuclear transcription factor-κB (NF-κB) and of signal transducer and activator of transcription 3 (STAT3). The expression of antiapoptotic, cell proliferation and tumor-promoting genes (<I>bcl-2</I>, <I>X-chromosome inhibitor of apoptosis protein</I> (<I>IAP</I>), <I>cellular IAP</I> and <I>cellular FADD-like IL-1β-converting enzyme-inhibitory protein</I>, <I>cyclin D</I>), cyclin-dependent kinase 4, cycolooxygenase-2 and inducible nitric oxide synthase was decreased, whereas the expression of apoptotic target genes (<I>caspase-3</I> and <I>-9</I>, <I>bax</I>) increased. In tumor, spleen and blood, the number of cytotoxic CD8<SUP>+</SUP> T cells and CD57<SUP>+</SUP> natural killer cells and the levels of IL-10 increased, but that of tumor necrosis factor-α (TNF-α), IL-1β and IL-6 decreased. We also found that forced overexpression of IL-32γ inhibited colon cancer cell (SW620 and HCT116) growth accompanied with the inhibition of activated NF-κB and STAT3 <I>in vitro</I>. In addition, when IL-32γ was knocked down by small interfering RNA (siRNA) or neutralized with an anti-IL-32γ antibody, IL-32γ-induced colon cancer cell growth inhibition, the IL-32γ-induced decrease of TNF-α, IL-1 and IL-6 production, and the increase of IL-10 production were abolished. However, siRNA of NF-κB and STAT3 augmented IL-32γ-induced colon cancer cell growth inhibition. These findings indicate significant pathophysiological roles of IL-32γ in cancer development.</P>

Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection

Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3

Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.

Combined steam and carbon dioxide reforming of methane and side reactions: Thermodynamic equilibrium analysis and experimental application

Jang, W.J.,Jeong, D.W.,Shim, J.O.,Kim, H.M.,Roh, H.S.,Son, I.H.,Lee, S.J. Applied Science Publishers 2016 APPLIED ENERGY Vol.173 No.-

<P>Thermodynamic equilibrium analysis of the combined steam and carbon dioxide reforming of methane (CSCRM) and side reactions was performed using total Gibbs free energy minimization. The effects of (CO2 + H2O)/CH4 ratio (0.9-2.9), CO2:H2O ratio (3:1-1:3), and temperature (500-1000 degrees C) on the equilibrium conversions, yields, coke yield, and H-2/CO ratio were investigated. A (CO2 + H2O)/CH4 ratio greater than 1.2, a CO2:H2O ratio of 1:2.1, and a temperature of at least 850 degrees C are preferable reaction conditions for the synthesis gas preparation in the gas to liquid process. Simulated conditions were applied to the CSCRM reaction and the experimental data were compared with the thermodynamic equilibrium results. The thermodynamic equilibrium results were mostly consistent with the experimental data, but the reverse water gas shift reaction rapidly occurred in the real chemical reaction and under excess oxidizing agent conditions. In addition, a long-term stability test (under simulated conditions) showed that the equilibrium conversion was maintained for 500 h and that the coke formation on the used catalyst was not observed. (C) 2016 Elsevier Ltd. All rights reserved.</P>

H₂ and CO production over a stable Ni-MgO-Ce_0.8Zr_0.2O₂ catalyst from CO₂ reforming of CH₄

Jang, W.J.,Jeong, D.W.,Shim, J.O.,Roh, H.S.,Son, I.H.,Lee, S.J. Pergamon Press ; Elsevier Science Ltd 2013 International journal of hydrogen energy Vol.38 No.11

<P>Ni-Ce0.8Zr0.2O2 and Ni-MgO-Ce0.8Zr0.2O2 catalysts were investigated for H-2 production from CO2 reforming of CH4 reaction at a very high gas hourly space velocity of 480,000 h(-1) Ni-MgO-Ce0.8Zr0.2O2 exhibited higher catalytic activity and stability (CH4 conversion >95% at 800 degrees C for 200 h). The outstanding catalytic performance is mainly due to the basic nature of MgO and an intimate interaction between Ni and MgO. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.</P>

A multi-microbe probiotic formulation processed at low and high drying temperatures: effects on growth performance, nutrient retention and caecal microbiology of broilers

Shim, Y.H.,Ingale, S.L.,Kim, J.S.,Kim, K.H.,Seo, D.K.,Lee, S.C.,Chae, B.J.,Kwon, I.K. Informa UK (TaylorFrancis) 2012 British poultry science Vol.53 No.4

<P>1. Two experiments were conducted to evaluate a multi-microbe probiotic formulation processed at low (LT) or high (HT) drying temperature. 2. In both the experiments, 640 d-old Ross male chicks were randomly allotted to 4 treatments on the basis of initial BW for 35 d experiments. 3. In experiment one, dietary treatments were a negative control (NC; basal diet without any antimicrobial); positive control (PC; basal diet +10?mg/kg avilamycin); basal diet with 03% probiotic LT; and basal diet with 03% probiotic HT. 4. Improved overall weight gain, FCR and retention of CP were observed in birds fed the PC and probiotic diets when compared with birds fed the NC diet. At d 21, birds fed the probiotic and NC diets had more caecal Bifidobacterium and total anaerobes than birds fed the PC diet; while birds fed the PC and probiotic diets had fewer caecal Clostridium than birds fed the NC diet at d 35. 5. In experiment two, a 2??2 factorial arrangement of treatments was employed to evaluate the effects of two concentrations of probiotic HT (030 or 060%) and avilamycin (0 or 10?mg/kg). 6. Birds fed the 060% probiotic HT diet showed improved overall weight gain and CP retention, higher Lactobacillus and Bifidobacterium in the caecum, and reduced Clostridium and coliforms in the caecum. Inclusion of avilamycin improved the overall weight gain and feed intake, and reduced the caecal Clostridium and Bifidobacterium population. 7. In conclusion, high drying temperature had no effect on the efficacy of the multi-microbe probiotic formulation; while the probiotic HT formulation was more effective at the 060% level. Moreover, inclusion of avilamycin improved performance of birds but did not have any interaction with probiotics.</P>

Pt-SnO_(2-x) 박막의 CO 가스 감지 특성

심창현,홍영호,박효덕,이천동,이덕동 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

Au 가 이산적으로 분포된 SnO₂_(-x) 박막의 가스감지 특성

심창현,정완영,박효덕,이재현,이덕동 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1

Sn thin film was deposited by thermal evaporation of Sn granulars. SnO_(2-x) thin film was obtained by thermal oxidation of the deposited Sn thin film. Au was deposited on the SnO_(2-x) and was annealed on 350℃ hot plate for 15 hours in air. Au thin film was dispersed as islands in this process. The dispersed gold film on tin oxide enhanced gas sensitivity to CO, C_(4)H_(10). Especially the SnO_(2-x) thin film had excellent selectivity for CO at low operating temperature.

Nontoxic outer membrane vesicles efficiently increase the efficacy of an influenza vaccine in mice and ferrets

Shim, S.M.,Song, E.J.,Song, D.,Lee, T.Y.,Kim, D.J.,Nam, J.H.,Gwin Jeong, D.,Lee, C.K.,Kim, S.H.,Kim, J.K. Elsevier Ltd. 2017 Vaccine Vol.35 No.30

In this study, we developed a further-modified outer membrane vesicle (fmOMV) from the ΔmsbB/ΔpagP mutant of Escherichia coli transformed with the plasmid, pLpxF, in order to use it as an adjuvant for pandemic H1N1 (pH1N1) influenza vaccine. We evaluated the efficacy of the pH1N1 influenza vaccine containing the fmOMV in animal models as compared to the commercial adjuvants, alum or AddaVax<SUP>TM</SUP>. The fmOMV-adjuvanted pH1N1 influenza vaccine induced a significant increase in the humoral immunity; however, this effect was less than that of the AddaVax<SUP>TM</SUP>. The fmOMV-adjuvanted vaccine displayed pronounced an enhanced protective efficacy with increased T cell immune response and reduced the viral load in the lungs of the infected mice after challenging them with a lethal dose of the homologous virus. Moreover, it resulted in a significantly higher cross-protection against heterologous virus challenge than that of the pH1N1 vaccine with alum or with no adjuvants. In ferrets, the fmOMV-adjuvanted vaccine elicited a superior antibody response based on the HI titer and efficiently protected the animals from the lethal viral challenges. Taken together, the nontoxic fmOMV could be a promising adjuvant for inducing robust T cell priming into the pH1N1 vaccine and might be broadly applicable to the development of preventive measures against influenza virus infection.

忠南錦江流域住民의 絲狀蟲에 關한 疫學的 調査硏究(第1報)

金鍾煥,沈雲澤,李泰淑,芮旻海,崔東河,朴元學 충남대학교 의과대학 지역사회의학연구소 1974 충남의대잡지 Vol.1 No.1

During June to November 1974, a survey of 1,749 (male 907, female 842) inhabitants for filariasis was made in several areas, Chung Cheong-nam Do, and the results are summerized as follows; 1. The positive rate of microfilaria was 0.17 per cent from Gongju(2 cases) and Ganggyeong(1 case), however no positive case of microfilaria was found in the inhabitants of Seosan area. 2. The microfilaria in per 20 cmm of blood showed lower density and it was about four worms. 3. 301 households in the surveyed areas were subjected for the survey releated with filariasis by the questionnaire. The family member of 172(57.1%) households showed 5 to 8 persons, agriculture 68.4%, and 71.8% of households lived more than 20 years in the same village. 4. 56.9% of household showed the protection from the mosquito biting with mosquito net or insecticides spray and 49.8% of residents knew the "Sudung-dari" dialet for elephantiasis, however some of them were missunderstood the cause of elephantiasis.