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RISS 인기검색어
- 검색키워드
- 저자 : Cun-Hong Pan (검색결과 3 건)
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Influence of Curcumin on HOTAIR-Mediated Migration of Human Renal Cell Carcinoma Cells
Pei, Chang-Song,Wu, Hong-Yan,Fan, Fan-Tian,Wu, Yi,Shen, Cun-Si,Pan, Li-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
Background: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)-mediated migration of cultured renal cell carcinoma (RCC) cells. Materials and Methods: Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to $80{\mu}mol/L$) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to $10{\mu}M$) on the migration of RCC cells was also determined. Results: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at $2.5-10{\mu}M$ had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner. Conclusions: HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.
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Characteristics of Carbohydrate Assimilation and Distribution in Walnut (Juglans regia L.)
Shi-Wei Wang,Cun-De Pan,Cui-Fang Zhang,Hong Chen 한국원예학회 2021 원예과학기술지 Vol.39 No.2
Based on <SUP>13</SUP>C isotope tracer technology, we investigated the <SUP>13</SUP>C abundance (δ<SUP>13</SUP>C), <SUP>13</SUP>C content(<SUP>13</SUP>Camount), and new fixed <SUP>13</SUP>C percentage (<SUP>13</SUP>CPCT) in different organs and different metabolic components in girdled fruit-bearing shoots at different stages of fruit growth and development in walnut. Our aim was to analyze the dynamic characteristics of carbohydrate assimilation, transportation, and utilization in different stages of walnut fruit growth and development. The results showed that (1) at 0 h after the feeding, δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the leaves were the highest and positively correlated with net photosynthetic rate of leaves (p < 0.05); (2) at 24 h after the feeding, δ13C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the carpopodium reached their peak values; (3) δ<SUP>13</SUP>C,<SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the peel were lower than those of hexose at 48 h after feeding in the fast-growing stage, but they all reached maximum levels in peel sucrose at 48 h after feeding in the oil conversion stage; and (4) the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in all carbohydrates from the seed kernel 48 h after feeding, among which the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in sucrose. These results suggested that sucrose was the main form of carbohydrate assimilation and transportation in walnut fruit, and large amounts of sucrose are accumulated in the peel and in the kernel, where it is also the base for the transformation of oil.
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