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Gene Expression of Cox5a, 5b, or 6b1 and Their Roles inPreimplantation Mouse Embryos1
Cui, Xiang-Shun,Li, Xing-Yu,Jeong, Yu-Jeong,Jun, Jin-Hyun,Kim,Nam-Hyung Society for the Study of Reproduction 2006 BIOLOGY OF REPRODUCTION Vol.74 No.3
To investigate the role of nuclear encoded genes in mitochondrialfunction during oocyte maturation and early embryogenesis weexamined the expression pattern and function of the cytochromeoxidase (Cox) subunits, Cox5a, 5b, and 6b1 during oocytematuration and early embryo development. Transcription of Cox5a,5b, or 6b1 was observed in oocytes and during early development;their expression levels were abundant in mature oocytes (MII) andzygotes (1C), and lowest at the 2-cell stage (2C), graduallyincreasing from 4-cell to blastocyst stage. Immunocytochemicalstudies revealed that COX5A, 5B, or 6B1 proteins were expressed inall blastomeres of the blastocyst. Silencing of mRNA expression byRNA interference (siRNA) did not inhibit oocyte maturation ordevelopmental events up to the morula and blastocyst stages, butdisrupted mitochondrial distribution. Significantly higherapoptosis and lower cell numbers were observed in siRNA-treatedblastocysts. Real time RT-PCR revealed that silencing of Cox5a,5b, or 6b1 did not alter mRNA levels of Bcl-xL (Bcl2l1), butincreased transcription levels of proapoptotic genes, Bax andcaspase 3 (Casp3). Furthermore, mRNA and protein levels ofE-cadherin (CDH1) were decreased in siRNA microinjectedblastocysts. These results suggest that gene expression of the Coxsubunits, Cox5a, 5b, and 6b1 is not required for embryodevelopmental events up to the blastocyst stage. The loss of thesegenes leads to mitochondrial dysfunction that results in apoptosisof the blastocyst stage embryos.
Yu Cui,Li Yong,Mo Rongli,Deng Wen,Zhu Zhixian,Hu Xingming 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.6
The ecological distribution and activities of soil microbes can refl ect the infl uence of environmental factors on microbes andchanges in soil fertility. Understanding the community structure of the soil microbes is of great importance to maintaining soilhealth in ancient mulberry plantation ecosystems. In this study, the population structure of soil microorganisms in diff erentages of mulberry plantations was analyzed using high-throughput sequencing technology. A total of 1616, 1568, 1379 and1477 operational taxonomic units were acquired from samples of 3Y (3-year-old), 35Y (35-year-old), 200Y (200-year-old)and 600Y (600-year-old) soils, respectively. The results exhibited that the highest community diversity was found in 35Ysoil, followed by 200Y soil, and showed that the stand age strongly infl uenced the community structure of soil bacteria. Atotal of 23 phyla were detected in 12 samples, and the genus of bacteria that predominated the diff erent stand ages mainlyincluded; Phenylobacterium , Cohnella , Lactococcus , Sphingomonas , Bacillus , and Microbispora . The relative abundanceof Phenylobacterium accounts for 22.6–53.5% of the total abundance. This study showed that the richness and diversity ofthe soil bacteria decreased as the stand age increased in ancient mulberry plantations.
( Yu Hua Cui ),( Ho Jung Choo ) 한국의류학회 2018 한국의류학회지 Vol.42 No.1
The contribution of foreign tourist consumption on national economies is becoming increasingly important for many countries. This study examines the relationship of home-host country psychic distance (HHCPD), tourism shopping motivation and attitudes towards local and global fashion brands in host countries. Survey data from a sample of 500 Chinese tourists were collected at an international airport in Korea. The results reveal that all three key factors of HHCPD (economic, geographic and cultural distance) positively affect social shopping motivation. Moreover, only economic distance has a positive effect on commodity shopping motivation, while both cultural and economic distance positively influence souvenir shopping motivation. All three factors of tourism shopping motivation positively affect local fashion brand attitudes, while only commodity shopping motivation positively affects consumer attitudes towards global fashion brands. Thus, fashion marketers should trigger the shopping motivation of foreign tourists by maximising the psychic distance from their home in order to satisfy fashion-seeking tourists.
Yu, Cui-Ge,Jiang, Xiang-Yang,Li, Bin,Gan, Lu,Huang, Jian-Feng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15
Background: To analyze the expression of estrogen receptors (ER), progesterone receptors (PR), C-erbB-2 and Ki-67 in endometrial carcinoma (EC) and their relationships with the clinicopathological features. Materials and Methods: Sixty-seven EC samples, 53 normal endometrial samples and 53 atypical hyperplasia endometrial samples were all selected in Shaanxi Provincial People's Hospital from Jun., 2012 to Jun., 2014. The expression of ER, PR, C-erbB-2 and Ki-67 in EC tissue, normal endometrial tissue and atypical hyperplasia endometrial tissue was respectively detected using immunohistochemical SP method. The relationships between the expression of ER, PR, C-erbB-2 and Ki-67 and the patients' clinicopathological features as well as their correlations in EC tissue were also analyzed. Results: The positive expression rates of ER and PR in EC tissue were 44.8% and 41.8%, respectively, dramatically lower than in atypical hyperplasia endometrial tissue and normal endometrial tissue (P<0.01). The positive expression rates of C-erbB-2 and Ki-67 in EC tissue were 80.6% and 64.2%, respectively, significantly higher than in atypical hyperplasia endometrial tissue and normal endometrial tissue (P<0.01). In EC tissue, the expression of ER and PR was closely associated with the differentiated degrees and depth of myometrial invasion (P<0.05), while that of C-erbB-2 and Ki-67 with the clinical staging, differentiated degrees, depth of myometrial invasion and presence or absence of lymph node metastasis (P<0.05). Spearman correlation analysis further displayed that the expression of ER was positively correlated with PR (r=0.393, P=0.001), but negatively with C-erbB-2 and Ki-67 (r=-0.469, P=0.000; r=-0.329, P=0.007); The expression of PR was negatively correlated with C-erbB-2 and Ki-67 (r=-0.273, P=0.025; r=-0.251, P=0.041), but that of C-erbB-2 positively with Ki-67 (r=0.342, P=0.005). Conclusions: Abnormal expression of ER, PR, C-erbB2 and Ki-67 might play an important role in endometrial malignant transformation and cell differentiation, so their joint detection is likely to be a comprehensive combination of immune factors, which is of great importance for EC prognosis.
Relational Benefits and Visual Arts Outcomes via WeChat Business
( Yu Hua Cui ) 한국패션비즈니스학회 2019 패션 비즈니스 Vol.23 No.6
The purpose of this study was to understand the effect of WeChat relational benefits on customer satisfaction and purchase intention. Due to the rapid development in the technological field, the use of WeChat has expanded drastically in recent years. In visual arts industry, WeChat business occupies a significant proportion of emerging industries. The recruited participants that were recruited via an online survey website were 335 (www.sojump.com). SPSS 22.0 statistical system was used to analyze the descriptive statistics, reliability, and validity. This study conceptualizes the positive relationship among relational benefits, customer satisfaction, and purchase intention of visual arts products. The findings indicated that WeChat users’ relational benefits can be categorized into two distinct benefit types: confidence, and social benefits. The more relational benefits WeChat users perceived, the higher the level of satisfaction. In contrast, the positive influence of customer satisfaction on purchase intention is weaker in consumers with a higher level of perceived risk. The results of this study will help WeChat business to retain its customers, thus, gaining a long-term value for visual arts industry. Theoretical and managerial implication for WeChat business are provided.