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Molecular characterization of the invasion genes of S. typhimurium
Park,Jeong Uck,Oh,Yang Hyo,Jeong,Yung Ki,Joo,Woo Hong,Coloe,Peter Jone 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.27 No.-
The potential invasion genes from S. typhimurium were identified by the construction of a cosmid library and subcloning the invasion genes into a plasmid vector, pGEM-7Z. The 4.65 kb fragment of the invasion-conferring genomic region of the subclone, pSV6235, which was derived from S. typhimurium, was sequenced in both direction. The genomic region showed several open reading frames. The three open reading frames, which were located at downstream of a promoter region, were designated as sir (Salmonella invasion region)A coding for the 36 amino acids, sirB coding for the 132 amino acids and sirC for the 82 amino acids, respectively. There was no significant nucleotide sequence homology between the sir genes of pSV6235 and the invasion genes which were characterized. Interesingly, the genomic region of pSV6235 was highly homologous to Yersinia enterocolitica genomic DNA for a high pathogenicity island and Salmonella enteritidis insertion element IS1351 and IS200 DNA. These results show that there could be a significant relationship between S. typhimurium, Y. enterocolitica and S. enteritidis with respect to horizontal evolution process and acquisition of virulence determinants by means of transposon, plasmid or bacteriophage.
Development of a biological delivery system for a live Salmonella vaccine
Park,Jeong Uck,Oh,Yang Hyo,Jeong,Yung Ki,Joo,Woo Hong,Coloe,Peter Jone 한국생명과학회 2000 한국생명과학회 학술발표회 Vol.27 No.-
A cosmid library of S. typhimurium was constructed in E. coli by transduction. Cultured HeLa and Chinese hamster ovary cells were infected by the cosmid library. The genomic region of a clone, pSI623 which was able to penetrate the mammalian cells was cleaved with BglII completely and subcloned into a plasmid vector, pGEM-7Z, resulting a series of subclones conserving the genomic region representing the invasion genes. The subclone, DH1(pSV6235) carrying 4.65 kb of the genomic region was highly able to penetrate HeLa and Chinese hamster ovary epithelial cells. In addition, the invasion efficiency of pSV6235 was significantly higher than that of DH1(pSI623). However, the subclones carrying 7.4 kb, 6.3 kb, 5.5 kb and 1.4 kb of the genomic region from pSI623 were not able to penetrate the mammalian cells. pSV6235 was introduced into a live Salmonella vaccine, S. dublin aroA mutant. The invasiveness of HeLa and Chinese hamster ovary epithelial cells by the vaccine strain harboring pSV6235 was significantly increased, compared with the vaccine strain. These results indicated that the non-invasive E. coli was significantly able to invade HeLa and Chinese hamster ovary epithelial cells by harboring the invasion genes of pSV6235 which were originally derived from S. typhimurium and could be applied as a biological delivery vector to augment immune efficacy.
동물세포로의 침입과 관련된 새로운 살모넬라 유전자의 발견
박정욱,정미연,김미림,주우홍,콜리, 피터 죤 Institute of Genetic Engineering Changwon National 1999 Gene and Protein Vol.3 No.1
To identify virulence genes involved in Salmonella infection, a Salmonella cosmid library was constructed in E. coli. The internalization efficiency of the Salmonella cosmid clones, pSI511 and pSI623 to HEp-2 and chinese hamster ovary cells was significantly increased, compared with the host strain, E. coli. This result illustrates that putative invasion genes on the S. typhimurium genomic DNA could be transferred into and expressed in noninvasive E. coli. The restriction enzyme analysis showed that the internalization-competent clones, DH1(pSI511) and DH1(pSI623) contained the different fragments derived from the Salmonella genomic regions. This result reflects that the invasion properties of S. typhimurium 82/6915 could be attributed by multiple genomic loci involved in the virulence.
Features and Efficiency of Some Platinized TiO₂ Photocatalysts
Emilio, Carina A.,Testa, Juan J.,Hufschmidt, Dirk,Colo´n, Gerardo,Navio´, Jose´ A.,Bahnemann, Detlef W.,Litter, Marta I. 한국공업화학회 2004 Journal of Industrial and Engineering Chemistry Vol.10 No.1
Three commercial powdered photocatalysts, Degussa P25 (P25), Sachtleben Hombikat UVlOO (WlOO) and Millennium TiONA PC 50 (PC50) have been platinized to incorporate 0.5 and 1 wt.% Pt on the surface of the particles. The physicochemical properties (SBET, particle size, morphology of the particles, etc.) have been measured and compared among the samples. The photocatalytic efficiency has been tested with two environmentally important oligocarboxylic acids, EDTA and NTA (5 mM). No strong differences could be observed among the catalysts in the case of EDTA, and an inhibition was observed in all cases. A striking effect of platinization has been observed in the case of NTA degradation, with a change in the kinetics from zero order in the pure precursors to first order in the platinized samples. Pure and platinized HB samples were the best photocatalysts for NTA. An optimum Pt amount (0.5 wt.%) was observed for the photoefficiency of the samples. It can be concluded that the efficiency is more dependent on the nature of the substrate to be degraded than on the physicochemical properties of the samples
The 72-h WEBT microvariability observation of blazar S5 0716 + 714 in 2009
Bhatta, G.,Webb, J. R.,Hollingsworth, H.,Dhalla, S.,Khanuja, A.,Bachev, R.,Blinov, D. A.,Bö,ttcher, M.,Bravo Calle, O. J. A.,Calcidese, P.,Capezzali, D.,Carosati, D.,Chigladze, R.,Collins, A.,Colo EDP Sciences 2013 Astronomy and astrophysics Vol.558 No.-