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        Knock out of the annexin gene OsAnn3 via CRISPR/Cas9-mediated genome editing decreased cold tolerance in rice

        Chunxiu Shen,Zhiqun Que,Yumei Xia,Ning Tang,Ding Li,Ronghua He,Mengliang Cao 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6

        Plant annexins are Ca2+-dependent phospholipidbindingproteins and exist as multigene families in plants. They are implicated in the regulation of plant developmentas well as protection from environmental stresses. In thisstudy, the rice annexin gene OsAnn3 knockout was performedvia the CRISPR/Cas9 (clustered regularly interspaced shortpalindromic repeats/CRISPR associated proteins) mediatedgenome editing. Thus, mutant plantlets were successfullyobtained. We identified cold tolerance phenotype of T1mutant lines from T0 biallelic mutants using the 4~6°C for 3days cold treatment. The results showed that REC (the relativeelectrical conductivity) of T1 mutant lines was increased, andthe survival ratio of T1 mutant lines was decreased dramaticallycompared with the wild type after the exposure to coldtreatment. It was suggested that OsAnn3 was involved in coldtolerance of rice.

      • An Image Retrieval Method Based on Visual Dictionary and Saliency Region

        Xu Gongwen,Xu Lina,Li Xiaomei,Tang Xiaobing,Wang Xiaoyan,Xu Chunxiu 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.7

        An image retrieval method based on the combination of visual dictionary and region of saliency was proposed in this paper, which aims to increase the accuracy of image retrieval. The image is divided into sampled blocks and the low-level features are extracted from these image blocks. Then a variety of features vectors are taken as the input vector for learning its corresponding visual dictionary respectively by non-negative sparse coding. Spatial information is added into the sparse representations of image by proposing the saliency polling method, and the similarity measure between sparse representation vectors is defined as SED (Squared Euclidean Distance), which considering the same nonzero entries and Euclidean distance of vectors at the same time. Results of experiment carried on Corel and Caltech datasets showed that this method can effectively improve the accuracy of image retrieval compared with the methods of single visual dictionary.

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        Controllable Synthesis of Silver Nanoparticles Using Piezoelectric-Actuated High-Frequency Vibration Self-Circulating Microfluidic Reactor

        Guojun Liu,Fang He,Yan Li,Xinbo Li,Hong Zhao,Conghui Wang,Conghong Zhan,Chunxiu Tang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.8

        Based on the liquid-phase reduction mechanism, a controllable synthesis method, which uses piezoelectric-actuated high-frequency vibration self-circulating microfluidic reactor, to prepare silver nanoparticles is proposed. Firstly, the synthesis mechanism of silver nanoparticles and the working principle of the microfluidic reactor were analyzed. Then, in order to study and explore the influence of self-circulating and high frequency vibration on the synthesis of silver nanoparticles, a series of related synthesis experiments were carried out. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy and transmission electron microscopy. The effects of micropump driving voltage and high-frequency vibration on the synthesis of silver nanoparticles were analyzed. The experiment results show that when the silver nanoparticles were synthesized using piezoelectric-actuated high-frequency vibration self-circulating microfluidic reactor, the higher the driving voltage of the circulating reflux micropump, the faster the vortex rotation speed in the mixing pool and the more uniform the reagent reaction. Besides, high-frequency vibration can suppress the aggregation of silver nanoparticles, and balance the growth environment of particles, which is beneficial to the formation of silver nanoparticles with good monodispersity, high sphericity and small size deviation.

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