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Chung, Yun-Jo,Kim, Kyung-Suk,Jeon, Eun-Soon,Park, Kie-In,Park, Chung-Ung Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.5
To investigate the effects of the Vitreoscilla hemoglobin (VHb) gene on the production of a heterologous protein, a comparative expression system for VHb and ferritin was constructed. First, the VHb gene was inserted into the downstream and upstream regions of the ferritin gene to construct pHF2 and pHF3, respectively. Next, the two plasmids pACHB1 and pVUTFH10, having the VHb gene and the ferritin gene respectively, were constructed in order to express the two genes in different plasmids by using a coplasmid expression system. It was observed that the cell growth was improved in all strains containing the VHb gene. Furthermore, in our coplasmid expression system, the presence of the VHb gene increased production of the ferritin by 1.8 times, as much as that in a strain not having the VHb gene.
사육사슴 및 야생고라니에서 소 세균성 전염병에 대한 혈청학적 연구
조영숙 ( Young Suk Jo ),정윤신 ( Yun Shin Chung ),소승영 ( Seung Young So ),설민숙 ( Min Suk Seol ),조호성 ( Ho Seong Cho ),김범석 ( Bumseok Kim ),임채웅 ( Chae Woong Lim ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
Deer can be one of the susceptible animals to bovine infectious diseases, and thus, may play a role either as a reservoir or amplifier host for spreading the diseases to other species such as cattle and goat. This study was conducted to determine the serum antibodies to bacterial infectious diseases for brucellosis, tuberculosis (TB), paratuberculosis (Johne`s disease) in deer. Serum samples were randomly collected from 78 deer from 31 farms at Jeonbuk province, and 7 wild water deer from Jeonbuk wild animal treatment center during 2005 to 2007, respectively. Four farm deer (5.1%) showed antibodies to tuberculosis using Antigen Rapid Bovine TB Ab Test Kit. One elk (1.3%) and one wild water deer had antibodies for paratuberculosis. Antibody against Brucellosis was not detected in tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA). These data suggest that caution should be applied to inspection of velvet, deer blood and meat for human consumption from deer because of zoonotic bacterial diseases in deer. In addition, farmed deer can be a transmissible host for zoonotic disease to diary or raising farm.
Jo, Myungjin,Chung, Ah Young,Yachie, Nozomu,Seo, Minchul,Jeon, Hyejin,Nam, Youngpyo,Seo, Yeojin,Kim, Eunmi,Zhong, Quan,Vidal, Marc,Park, Hae Chul,Roth, Frederick P.,Suk, Kyoungho Cold Spring Harbor Laboratory Press 2017 Genome Research Vol. No.
<P>To understand disease mechanisms, a large-scale analysis of human yeast genetic interactions was performed. Of 1305 human disease genes assayed, 20 genes exhibited strong toxicity in yeast. Human yeast genetic interactions were identified by en masse transformation of the human disease genes into a pool of 4653 homozygous diploid yeast deletion mutants with unique barcode sequences, followed by multiplexed barcode sequencing to identify yeast toxicity modifiers. Subsequent network analyses focusing on amyotrophic lateral sclerosis (ALS)-associated genes, such as optineurin (OPTIV) and angiogenin (ANG), showed that the human orthologs of the yeast toxicity modifiers of these ALS genes are enriched for several biological processes, such as cell death, lipid metabolism, and molecular transport. When yeast genetic interaction partners held in common between human OPTN and ANG were validated in mammalian cells and zebrafish, MAP2K5 kinase emerged as a potential drug target for ALS therapy. The toxicity modifiers identified in this study may deepen our understanding of the pathogenic mechanisms of ALS and other devastating diseases.</P>
정의식,강형모,류봉조,임경빈,고준빈,박수규 한밭대학교 산업과학기술연구소 1995 논문집 Vol.3 No.-
This paper deal with a method of detecting localized defects on taper roller bearing in main spindle of machine tool system. The statistical parameters in time-domain signal processing techniques have been calculated to extract useful features from bearing vibration signals. Emloying these features, the statiscical parameters have been established to detect defects on the rollers of bearings, respectively, and used by the input feature of an artificial neural nerwork. As a result that the proposed bearing defects monitoring system could be successfully performed by using the statistical parameters of acceleration signals.
Surface-diffusion-limited growth of atomically thin WS2 crystals from core-shell nuclei
Jo, Sunghwan,Jung, Jin-Woo,Baik, Jaeyoung,Kang, Jang-Won,Park, Il-Kyu,Bae, Tae-Sung,Chung, Hee-Suk,Cho, Chang-Hee The Royal Society of Chemistry 2019 Nanoscale Vol.11 No.18
<P>Atomically thin transition metal dichalcogenides (TMDs) have recently attracted great attention since the unique and fascinating physical properties have been found in various TMDs, implying potential applications in next-generation devices. The progress towards developing new functional and high-performance devices based on TMDs, however, is limited by the difficulty in producing large-area monolayer TMDs due to a lack of knowledge of the growth processes of monolayer TMDs. In this work, we have investigated the growth processes of monolayer WS2 crystals using a thermal chemical vapor deposition method, in which the growth conditions were adjusted in a systematic manner. It was found that, after forming WO3-WS2 core-shell nanoparticles as nucleation sites on a substrate, the growth of three-dimensional WS2 islands proceeds by ripening and crystallization processes. Lateral growth of monolayer WS2 crystals subsequently occurs by the surface diffusion process of adatoms toward the step edge of the three-dimensional WS2 islands. Our results provide understanding of the growth processes of monolayer WS2 by using chemical vapor deposition methods.</P>
Chung, Won-Suk,Nam, Dong-Hyun,Jo, Dae-Jean,Lee, Jun-Hwan The Korean Neurosurgical Society 2011 Journal of Korean neurosurgical society Vol.49 No.1
Objective: Ossification of the posterior longitudinal ligament (OPLL) has a strong genetic component. Specific gene polymorphisms may be associated with OPLL in several genes which regulate calcification in chondrocytes, change of extracellular collagen matrix and secretions of many growth factors and cytokines controlling bone morphogenesis. Toll-like receptor 5 (TLR5) may playa role in the pathogenesis of OPLL by intermediate nuclear factor-kappa B (NF-${\kappa}B$). The current study focused on coding single nucleotide polymorphisms (SNPs) of TLR5 for a case-control study investigating the relationship between TLR5 and OPLL in a Korean population. Methods: A total of 166 patients with OPLL and 231 controls were recruited for a case-control association study investigating the relationship between SNPs of TLR5 gene and OPLL. Four SNPs were genotyped by direct sequencing (rs5744168, rs5744169, rs2072493, and rs5744174). SNP data were analyzed using the SNPStats, SNPAnalyzer, Haploview, and Helixtree programs. Multiple logistic regression analysis with adjustment for age and gender was performed to calculate an odds ratio (OR). Results: None of SNPs were associated with OPLL in three alternative models (codominant, dominant, and recessive models; p> 0.05). A strong linkage disequilibrium block, including all 4 SNPs, was constructed using the Gabriel method. No haplotype was significantly associated with OPLL in three alternative models. Conclusion: These results suggest that Toll-like receptor 5 gene may not be associated with ossification of the posterior longitudinal ligament risk in Korean population.
Intertypic variations of 6 kinds of bristles on male genitalia in Drosophila auraria Peng
Chung, Yong Jai,Oh, Suk Hung,Rho, Boon Jo 이화여자대학교 한국문화연구원 1959 韓國文化硏究院 論叢 Vol.1 No.-
1. D. auraria Peng은 A, B, C 3型으로 나눌 수 있는데 本種은 分類學的 및 集團遺傳學的硏究의 좋은 材料이다. 2. Drosophilidae의 male genitalia는 分類學的硏究의 重要한 標徵으로 되어 있다. 3. 著者들은 1957年 5月부터 1959年 9月까지 南韓 8個處에서 採集된 drosophilid fly 中의 本種 3型의 genitalia slide preparation 173枚를 作成하여 그 6種의 剛毛 卽 1) anal plate bristle, 2) 그 tip teeth, 3) genital arch의 bristle, 4) primary clasper의 primary teeth, 및 5) marginal teeth, 와 6) secondary clasper의 teeth 數를 觀察하여 이들의 3型間에 있어서의 有意의 差를 檢定調査하고 이들이 3型을 區別하는 좋은 criteria가 될는지의 如否를 檢討하였다. 4. anal plate의 tip teeth는 三型間에 서로 有意의 差는 完全히 볼 수 없었고, 그 他에 있어서는 A, B, C 中 어느 2型間에서는 그 有意의 差를 認定할 수 있으나 3型間에 있어서의 뚜렷한 有意의 差는 볼 수 없었으니 要는 本硏究에서 取扱되는 6種의 形質의 3型間에 있어서의 뚜렷한 有意의 差는 볼 수 없으며 따라서 이들이 3型을 分類하는 좋은 criteria는 못된다고 본다. 本硏究結果에서 나타난 剛毛數는 Hsu(1949)의 記錄과 若干의 差異를 나타내고 있다. 5. 本硏究는 다음과 같은 未備漢 點이 있으니 卽 本硏究에서 取扱된 A型(47)과 B型(26)이 C型에 比하여 훨씬 적었다는 것(採集個體不足으로 因하여). 採集地에 따르는 有意性 檢定을 못했다는 點, 그리고 交難實驗等으로 集團遺傳學的 問題를 追究하지 않았다는 點 等인데 이런 問題를 檢討補充하는 硏究가 계속될 것이 要望된다. Intertypic Variations of 6 kinds of Bristles on male genitalia in Drosophila auraria Peng / Y.J. Chung, S.H. Oh, B.J. Rho D. auraria Peng is divided into 3 types and is a significant species in surveys of population systematics and population genetics. Male genitalia of this species is a important criteria for taxonomy. The authors observed 6 kinds of bristles or teeth: bristles on anal plate, tip teeth on anal plate, bristles on genital arch, primary teeth and marginal teeth on primary cilasper and teeth on secondary clasper of 47 members of Type A, 26 of B and 100 of C of the species chosen from the alcoholic specimens collected in nature during a period ranging from May 1957 to September 1959 or from the stocks maintained in the genetic laboratory, Dept. of Biology, Ewha Womans University to examine statistically the significant differences of them among 3 types and also to discuss whether they will be possible to be the criteria for intertypical classification or not. As the results of observations, intertypic differences of tip teeth on anal plate are not significant at all. In others, even though there occurred significant differences between some 2 types, are also insignificant among 3 types. In brief, significant differences of bristles or teeth concerned in the present works are not distinct intertypically, consequently these quantitative characters are not be regard as a clear out criteria for classification of the types of D. auraria Peng. By the way, there are slight differences between Hsu's descriptions (1949) of the bristles or teeth concerned here and the present results.