자외선의 조사간격이 브로일러 병아리의 볏 피부중 비타민 D₃함량에 미치는 영향

조인호,장윤환,이은택,여영수,배은경,김중달 한국축산학회 1994 한국축산학회지 Vol.36 No.1

This study was conducted to determine the content, of previtamin D₃(PreD₃), lumisterol₃(L₃), vitamin D₃(VD₃) and provitamin D₃(ProD₃) in comb skski of broiler chicks exposed to medium ware ultraviolet(UVB) lights in different interval. The broiler Hubbard line day old chicks(199 = 10 control + 3 irradiation interval × 9 elapsed time × 7 replica) were fed vitamin D deficient diet for 3 weeks in a windowless subdued light room and exposed to 297 ㎚ UVB light by 0.068 mJ/㎝-(10 min) three times in 0, 12 or 24 h interval. The comb skin were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, and epidermis and dermis were separated. The lipid in sample was extracted by 9% ethyl acetate/hexane and purified by Sep-Pak silica catridge. The stright phase HPI-C was applied to analyze the concentration of Prop; and its photoproducts. When chicks were exposed once to UVB light for 30 min without interval, the mole % of ProD₃ in comb epidermis were 100% at control and 52.65% at 0 h after irradiation, thereafter it increased gradually to 88.17% at 240 h. PreD₃ and L₃ presented the maximum mole % at 0 h. VD₃ showed the peak value at 12 h. then decreased slowly. As UVB light was utilized to irradiate the chicks for 10 thin three times in 12 h interval, the ProD₃ mole portion in epidermis at 0 h was 76.4%, the lowest value among tested. PreD₃ and 1-3 preserved the highest level at 24 and 0 h, respectively, thereafter decreased gradully. VD₃ showed a peak at 6 h after exposure. When 24 h interval system was treated, the lowest value of ProD₃ 83.52% was appeared at 0 h. PreD₃ and L3 showed the highest level at 6 and 0 h, respectively. Mole ale of VD₃ had a peak value at 6 h and thin decreased. The mole % of ProD₃ and its photoproduets in comb dermis presented similar trends of time course variation as in those in epidermis. In respecting the method of UVB irradiation the PreD₃, L, and VDT were produced more quickly and largely in no intend system as compared to the time and amount produced in 12 or 24 h interval system.

VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5

Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.

돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

최봉환,김태헌,이지웅,조용민,이혜영,조병욱,정일정 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of β-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F_(2) population composed of 214 individuals form an intercross between Korean Native Boars and Landrace sows. PCR products form tow primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and Hinf Ⅰ, revealed fragment length polymorphisms(RFL. Ps). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP(hinf Ⅰ was .38, .41, and .20, respectively, in the population.Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weight. In H-FABP/Hinf Ⅰ Iocus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p<.05 or p<.001) and back fat thickness, body fat including abdominal and trimmed fat (p<.001) and intramuscular fat(p<.05). The 'H'allele was positivecly associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

열처리에 따른 Y₂O₃박막의 미세 구조 변화와 전기적 특성 변화에 대한 고찰

정윤하(Y. H. Jung),강성관(S. K. Kang),김은하(E. H. Kim),고대홍(D. H. Ko),조만호(M. H. Cho),황정남(C. N. Whang) 한국진공학회(ASCT) 1999 Applied Science and Convergence Technology Vol.8 No.3(1)

p-type Si (100) 기판 위에 습식 산화법으로 SiO₂ 층을 형성한 후, Ionized Cluster Beam(ICB) 증착 방법으로 200 Å 두께의 Y₂O₃ 박막을 증착 하였다. Y₂O₃ 박막이 증착 된 시편을 산소, 아르곤 분위기에서 열처리한 후, Atomic Force Microscopy(AFM)과 Transmission Electron Microscopy(TEM)을 사용하여 Y₂O₃ 박막의 표면과 계면을 관찰하였다. 열처리를 수행한 후 Y₂O₃ 박막과 Si 기판 사이에서 SiO₂층이 성장하고, 이트륨실리 케이트 층이 형성되는 것을 관찰할 수 있었다. 산소 분위기에서 열처리를 수행한 시편의 Y₂O₃ 박막 표면을 관찰한 결과, 표면 상부에 아르곤 분위기에서 열처리한 시편에서는 보이지 않았던 새로운 상이 형성되었음을 알 수 있었다. 또한 전기적 특성을 측정하기 위하여 Al/Y₂O₃/p-type Si (100) 의 캐패시턴스-전압 특성을 관찰하였고, 그 결과 Y₂O₃ 박막의 유전 상수 값이 약 9정도임을 알 수 있었다. We investigated the interfacial reactions between the Y₂O₃ film deposited by ICB processing and p-type (100) Si substrates upon annealing treatments in O₂ and Ar gas ambients. We also investigated the evolution of surface morphology of ICB deposited Y₂O₃ films upon annealing treatments. We observed that the root-mean-square (RMS) value of surface roughness measured by AFM increased with annealing time at 800℃ in O₂ ambient, while the change of surface roughness was not observed in Ar ambient. We also found the growth of SiO₂ layer and the formation of yttrium silicate layer. From the capacitance values (C_(acc)) measured by C-V measurements, the relative dielectric constant of Y₂O₃ film in metal-insulator-semiconductor (MIS) structure was estimated to be about 9.

Coumarins reduce biofilm formation and the virulence of Escherichia coli O157:H7

Lee, J.H.,Kim, Y.G.,Cho, H.S.,Ryu, S.Y.,Cho, M.H.,Lee, J. G. Fischer 2014 Phytomedicine Vol.21 No.8

E. coli O157:H7 is the most common cause of hemorrhagic colitis, and no effective therapy exists for E. coli O157:H7 infection. Biofilm formation is closely related to E. coli O157:H7 infection and constitutes a mechanism of antimicrobial resistance. Hence, the antibiofilm or antivirulence approach provides an alternative to antibiotic strategies. Coumarin and its derivatives have a broad range of biological effects, and in this study, the antibiofilm activities of nine coumarins were investigated against E. coli O157:H7. Coumarin or umbelliferone at 50μg/ml was found to inhibit biofilm E. coli O157:H7 formation by more than 80% without affecting bacterial growth. Transcriptional analysis showed that coumarins repressed curli genes and motility genes in E. coli O157:H7, and these findings were in-line with observed reductions in fimbriae production, swarming motility, and biofilm formation. In addition, esculetin repressed Shiga-like toxin gene stx2 in E. coli O157:H7 and attenuated its virulence in vivo in the nematode Caenorhabditis elegans. These findings show that coumarins have potential use in antivirulence strategies against persistent E. coli O157:H7 infection.

자외선의 상이한 선량이 브로일러 병아리의 중족골 광물질 함량에 미치는 영향

조인호,장윤환,여영수,강훈석,김강수,배은경 한국축산학회 1994 한국축산학회지 Vol.36 No.2

This study was carried out to investigate the concentrations of Ca, P and ash in the metatarsus of broiler chicks exposed to 0.204 mJ/㎠ 30 min) or 0.409 mJ/㎠(60 min) of medium wave ultraviolet(UVB) fight. Eighty two Hubbard dayold broiler chicks(2 doses × 9 elapsed times × 4 replicate + 10 negative controls) were fed vitamin D(VD)deficient diets for 31 days in a windowless subdued light room. The metatarsus were collected at 0, 6, 12, 18, 30, 42, 66, 90, or 138 h after UVB irradiation. The metatarsus bones were separated from adhering tissue, ether extracted, dried and ashed(A.O.A.C., 1984). The Ca content in non-fat dry metatarsus was measured by atomic absorption spectrophotometry and P by urnmonium metavanadate colorimetry. The mean Ca concentration in metatarsus of negative control birds was 21 %, and those of chicks exposed to UVB light for 30 and 60 min were 30 and 38%, respectively with the maximum values at 42 h after irradiation. Thereafter, the Ca contents decreased throughout the l38 h period of this research. The mean P level of control chicks was 8.5%, and the level peaked at 15% (30 min treatment) and 11%(60 min) at 66 h after exposure. The decreasing trend continued later than 66 h. This mean ash content of the control group was 43% with the ash contents of the 0.204 mJ/㎠(30 min) and 0.409 mJ/㎠(60 min) groups decreasing slightly until 42 h, but increasing to 46%(30 min) and 47%(60 min) at l38 h after irradiation. This verified that Ca and P contents increased first and ash amount increased later in metatarsal bones of broiler chicks when they were exposed to 0.204 and 0.409 mJ/㎠ UVB light. Since there was not a big difference between minerals in metatarsal bones of chicks exposed to 0.204 or 0.409 mJ/㎠ UVB light, it was thought that further experiments would be necessary to elucidate the optimum dose of UVB light less than 0.204 mJ/㎠.

The antimicrobial peptide human cationic antimicrobial protein-18/cathelicidin LL-37 as a putative growth factor for malignant melanoma

Kim, J.E.;Kim, H.J.;choi, J.M.;Lee, K.H.;Kim, T.Y.;Cho, B.K.;Jung, j.Y.;Chung, K.Y.;Cho, D.;Park, H.J. Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Background Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. Objectives To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Methods Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Results Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immuno- histochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Conclusions Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.

Sodium-ion supercapacitors based on nanoporous pyroproteins containing redox-active heteroatoms

Cho, S.Y.,Yoon, H.J.,Kim, N.R.,Yun, Y.S.,Jin, H.J. Elsevier Sequoia 2016 Journal of Power Sources Vol.329 No.-

Nanostructured carbon-based materials fabricated via simple methods from renewable bio-resources have great potential in rechargeable energy storage systems. In this study, nanoporous pyroproteins containing a large amount of redox-active heteroatoms (H-NPs) were fabricated from silk fibroin by an in situ carbonization/activation method. The H-NPs have a large surface area of ~3050 m<SUP>2</SUP> g<SUP>-1</SUP>, which is mainly comprised of nanometer-scale pores. Also, these H-NPs have oxygen and nitrogen heteroatoms of 17.4 wt% and 2.9 wt%, respectively. Synergistic sodium ion storage behaviors originate from electrochemical double layer capacitance and pseudocapacitance, leading to very high electrochemical performances of H-NPs in aqueous and non-aqueous electrolyte systems. Sodium-ion supercapacitors (NISs) based on commercial graphite//H-NPs show a high specific power of ~1900 W kg<SUP>-1</SUP> at ~77 Wh kg<SUP>-1</SUP>. Also, NISs based on commercial hard carbon//H-NPs exhibit a high specific energy of ~217 Wh kg<SUP>-1</SUP> at ~42 W kg<SUP>-1</SUP>. In addition, outstanding cycling performances over 30,000 cycles are achieved for symmetric NISs.

볏짚의 사료가치 증진을 위한 적정 알카린 H2O2 처리 수준에 관한 연구

문양수(Y . S . Moon),하종규(J . K . Ha),고종열(J . Y . Ko),최연호(Y . H . Choy),조경훈(G . H . Cho),최윤재(Y . J . Choi),한인규(I . K . Han) 한국축산학회 1990 한국축산학회지 Vol.32 No.10

This study was carried out to determine the adequate levels of alkaline hydrogen peroxide treatment for the improvement of nutritive value of rice straw. In vitro digestibility and chemical analysis after several treatments were measured. Treatment variables were soaking time(12, 24, 48 and 96 hrs), temperature(5, 25, 50 and 75℃) of alkaline H₂0₂ solution, H₂0₂ concentration (0.5, 1, 2, 3 and 4%) and substrate /solution ratio (1, 2, 3, 4 and 5g/ml). The results obtained were summarized as follows. 1. The DM digestibilities or rice straw treated with H₂O₂ for 24 and 48 hours were higher than those of the others(p$lt;0.05). There was no differ ences when the rice straw was treated for 12, 72 or 96 hours. Cell wall contents were not affected by the time of treatment. 2. The DM digestibilities were higher at pH 11.5 or above(p$lt;0.05) and was decreased as pH declined. When rice straw was treated with H₂O₂ at pH 11.5 or higher, NDF, ADF and cellulose contents were increased. However, lignin content and DM recovery percentages were decreased. 3. The DM digestibilities were not influenced by the ratio of straw versus liquid. The contents of NDF and cellulose were not affected up to the ratio of 4g rice straw/50m1, but were decreased at 5g rice straw/50m1. Lignin content and DM recovery percentages, however, were increased at 5g rice straw /50m1. 4. The DM digestibilities were not different among treatments at 5, 25 and 50℃. However, the rice straw treated at 75℃ showed lowest digestibility of all treatment (p$lt;0.05). The content of NDF, ADF and cellulose was increased by increasing temperature. However, lignin content and DM recovery percentages were decreased by increasing temperature. 5. The AHP treated rice straw had higher content of ADF and cellulose, and lower content of hemicellulose and lignin as the concentration of H₂O₂, increased. But hemicellulose, lignin and DM recovery percentages were decreased. The NDF content was not different among five different concentrations of H₂0₂ solution. In conculsion, based upon the results of present experiments the most desirable method is to soak rice straw in 1% alkaline H₂0₂ solution at pH 11.5, at room temperatrve (25℃), for 24∼48 hours and at the ratio of 4g rice straw /50m1 solmtion.

PEMFC 용 Pt 담지 촉매의 Pt 담지비에 따른 성능변화

조용훈(Y. H. Cho),조윤환(Y. H. Cho),박현서(H. S. Park),성영은(Y. E. Sung) 한국정밀공학회 2006 한국정밀공학회 학술발표대회 논문집 Vol.2006 No.5월

This study focuses on a determination of amount of Pt in the Pt/C for catalysts of polymer electrolyte membrane fuel cells (PEMFC). PEMFC offer low weight and high power density and being considered for automotive and stationary power applications. The PEMFC behavior is quite complex is influenced by several factors, including catalysts and structure of electrode and membrane type. Catalyst of electrode is important factor for PEMFC. One of the obstacles preventing polymer electrolyte membrane fuel cells from commercialization is the high cost of noble metals to be used as catalyst, such as platinum. To effectively use these metals, they have to be will dispersed to small particles on conductive carbon supports. The optimal amount of Pt in Pt/C was investigated by using polarization curves in single cell with H₂/O₂ operation.