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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Cloning of Two chitin Synthase Gene Fragments from Penicillium diversum
Cho, Seong-Pil,Lee, Sang-Keun,Lee, Dong-Hun,Bae, Kyung-Sook,Park, Hee-Moon,Maeng, Pil-Jae 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
The PCR fragments of two distinct chitin synthase genes, PdCHS1 and PdCHS2, were cloned from Penicillium diversum KCTC 6786. The nucleotide sequences of PdCHS1 and PdCHS2 contained uninterrupted open reading frames (ORFs) of 570 bp excluding the prime sequence. The similarity analysis of the deduced amino acid sequences using BLASTP indicated that the possible evolutionary relationship between P. diversum and ascomycetous fungi. Multialignment of the deduced amino acid sequences of PdCHSs using CLASTAL W revealed that the PdCHSs fell into two different classes: PdCHS1 into Class I and PdCHS2 into Class Ⅱ of chitin synthase defined by Bowen et al. (1992). By Southern blot analysis, it was shown that each of the two genes is present as a single copy in the genome of P. diversum KCTC 6786.
Cho, Keun Hwi,Suk, Sung Dae,Yeoh, Yun Young,Li, Ming,Yeo, Kyoung Hwan,Kim, Dong-Won,Park, Donggun,Lee, Won-Seong,Jung, Young Chai,Hong, Byung Hak,Hwang, Sung Woo IEEE 2007 IEEE electron device letters Vol.28 No.12
<P> The characteristics of cylindrical gate-all-around twin silicon nanowire field-effect transistors with a radius of 5 nm have been measured in temperatures <TEX>$T$</TEX> ranging from 4 to 300 K. The <TEX>$T$</TEX> dependence of the off-current suggests that thermal generation in the channel is the main leakage mechanism. The <TEX><TEX>$T$</TEX> </TEX> dependence of the subthreshold swing exhibits no body effects but shows degradations due to slight differences in the threshold voltages and in the body effect constants of the twin nanowires. The <TEX>$T$</TEX> dependence of the peak normalized transconductance <TEX>$g_{m}/V_{\rm DS}$</TEX> gives a clue of 1-D phonon scattering and suggests that surface roughness scattering at the nanowire wall is dominant at low <TEX>$T$</TEX> values. </P>
Cho, Young-Seok,Chae, Hiun-Suk,Kim, Hyung-Keun,Kim, Jin-Soo,Kim, Byung-Wook,Kim, Sung-Soo,Han, Sok-Won,Choi, Kyu-Yong WJG Press 2008 WORLD JOURNAL OF GASTROENTEROLOGY Vol.14 No.13
<P>To compare the hemostatic efficacy and safety of two mechanical endoscopic methods: endoscopic band ligation (EBL) and endoscopic hemoclip placement (EHP) in patients with actively bleeding Mallory-Weiss syndrome (MWS).</P>
Cho, Sang-Hwan,Oh, Jeong Rok,Park, Hoo Keun,Kim, Hyoung Kun,Lee, Yong-Hee,Lee, Jae-Gab,Do, Young Rag The Optical Society 2010 Optics express Vol.18 No.2
<P>We demonstrate the combined effects of a microcavity structure and light-recycling filters (LRFs) on the forward electrical efficiency of phosphor-converted white organic light-emitting diodes (pc-WOLEDs). The introduction of a single pair of low- and high-index layers (SiO(2)/TiO(2)) improves the blue emission from blue OLED and the insertion of blue-passing and yellow-reflecting LRFs enhances the forward yellow emission from the YAG:Ce(3+) phosphors layers. The enhancement of the luminous efficacy of the forward white emission is 1.92 times that of a conventional pc-WOLED with color coordinates of (0.34, 0.34) and a correlated color temperature of about 4800 K.</P>
POSTMORTEM STUDY ON A KOREAN WOMAN WITH CEREBRAL RETE MIRABILE
Cho, Maeng Ki,Shin, Dong Whi,Rhee, Hyun Jae,Chae, Jin,Choi, Kil Soo,Sim, Bo Sung,Lee, Hyun Soon,Ham, Eui Keun 대한신경외과학회 1973 Journal of Korean neurosurgical society Vol.2 No.2
환자는 47세 된 한국 여자로서, 입원하기 5일전 목욕탕에서 무의식 상태로 발견된 후 혼수상태로 서울대학교 의과대학 부속병원 신경외과에 입원했다. 환자는 10년전에도 비슷한 병력을 가지고 있었으며 약 10일간의 무의식 상태에서 특별한 후유증없이 회복되어 그후 비교적 정상적인 생활을 해 왔다. 입원후 우측 경동맥조영술을 시행한바 전대뇌동맥이 전혀 보이지 않았으며, 정중대뇌동맥의 병적인 미세한 분지가 관찰되었다. 뇌척수액검사는 지주막하출혈의 소견을 보이고 있었다. 환자는 EACA, Steroid 등으로 치료받아 첫 일주일간 병세가 호전되어 의사표시를 할 수 있게 되었으나 입원 12일째부터 갑자기 고열이 나면서 악화되어 약 10일간 고열이 계속되다가 제21병일에 사망했다. 사망 13시간후 시행한 부검소견을 보면, 육안적으로 우측 뇌실 및 기저핵부에 혈종이 관찰되었으며 Willis환 전반부에서의 불규칙한 현관주행을 볼 수 있었다. H&E 및 Verhoeff 염색법에 의한 현미경적 소견은 지금까지 알려진 전형적인 소견 즉 동맥내경의 Polster 양비후가 전반적으로 관찰되었고 동맥벽에서의 염증성 소견은 볼 수 없었다. 뇌실질내혈종주위의 염증성 소견은 2차적인 것으로 보여진다. 저자들은 본 증례를 보고하면서 지금까지 알려진 지식 이외의 특별한 기여를 하지는 못했으나 일본인 이외의 환자로서는 처음으로 부검례를 보고한다는 점에 그 의의를 찾고자 한다.
In-hospital mortality in patients with STEMI admitted during off hours
( Sung-Soo Kim ),( Myung Ho Jeong ),( Jeong Gwan Cho ),( Young Keun Ahn ),( Jong Hyun Kim ),( Shung Chull Chae ),( Young Jo Kim ),( Seung Ho Hur ),( In Whan Seong ),( Taek Jong Hong ),( Dong-hoon Choi 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1
Conflicting results exist on the outcome of off hours PCI in ST elevation myocardial infarction (STEMI). However, there were only a few studies that have focused on the clinical characteristics and outcomes of off hours PCI in STEMI. So, we studied the clinical characteristics and hospital mortality in STEMI patients treated with primary PCI during regular hours (weekdays 9:00 AM to 6:00 PM) versus off hours Weekdays 6:01 PM to 8:59 AM, weekends, and holidays) in Korea Acute Myocardial Infarction Registry. We analyzed in hospital and one year mortality among 5,665 consecutive ST segment elevation myocardial infarction patients treated with primary PCI between November 2005 to January 2008. Total 2,848 (50.2%) patients were treated during off hours. Baseline finding were similar, although regular hours patients were older. Median symptom to balloon time (304 min, IQR 175 to 750 vs. 270 min, IQR 145 to 551, p=0.001) were longer for regular hours primary PCI. Median door to balloon time (71min, IQR: 48 to 132 vs. 59 min, IQR 39 to 110 min, p=0.001) were longer for off hours pPCI. Also, Cardiac enzyme such as Max CK-MB (212.1±299.3 vs 194.7±303.4, p=0.031) and max TnI (72.6±239.5 vs. 58.9±94.4, p=0.013) were increased in off hours pPCI. However, unadjusted in hospital (6.0% off hours vs. 6.0% regular hours, p=0.946) and one year cardiac mortality (11.3% off hours vs. 11.7% regular hours, p=0.661) were comparable. In multivariate analysis, off hours primary PCI did not predict an adverse outcome. In conclusion, when primary PCI was performed within an appropriate reperfusion strategy, the clinical effectiveness of either off hours or regular hours pPCI is comparable.
Case Reports : A Case of Hereditary Angioedema Associated with Idiopathic Hypoparathyroidism
Sang Hoon Kim,Byung Jae Lee,Yoon Seok Chang,Yoon Keun Kim,Sang Heon Cho,Kyung Up Min,You Young Kim 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.4
Hereditary angioedema is a rare autosomaldominant disease characterized by the edema of subcutaneous tissues, respiratory tract and bowel. It is caused by the deficiency of C1 esterase inhibitor. Hereditary angioedema may be associated with autoimmune dise