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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Cho, Dae-Hyun,Lee, Sung-Ae,Lee, Young-Ze Taylor Francis 2009 Tribology transactions Vol.53 No.1
<P> Two methods are proposed to reduce friction losses and the amount of wear in a piston assembly. One is the modification of the surface roughness of the skirt. Surface coating is another method to protect the sliding surfaces. In order to study the roughness of the skirt surfaces, the surfaces were ground to have three different roughness values. Graphite and diamond-like carbon (DLC) were also used to coat the surfaces of the piston skirts, while the specimens used in the test were the skirt and the cylinder bores from a low-friction diesel engine. These were tested with a reciprocating wear tester that used a piece of cylinder block as the reciprocating specimen and a segment of the piston skirt material as the fixed pin. SAE5W40 engine oil was used in the boundary lubrication regime. The friction coefficient of the graphite coating was lower than the DLC coating, but the graphite coating was not effective in protecting the wearing of the surfaces. The DLC coating provided much better tribological performance than the graphite coating.</P>
Cho, Jung‐,Il,Burla, Bo,Lee, Dae‐,Woo,Ryoo, Nayeon,Hong, Soon‐,Kwan,Kim, Hyun‐,Bi,Eom, Joon‐,Seob,Choi, Sang‐,Bong,Cho, Man‐,Ho,Bhoo, Seong Hee,Hahn, Tae Blackwell Publishing Ltd 2010 The New phytologist Vol.186 No.3
<P><B>Summary</B></P><P><P>In Arabidopsis, the compartmentation of sugars into vacuoles is known to be facilitated by sugar transporters. However, vacuolar sugar transporters have not been studied in detail in other plant species.</P><P>To characterize the rice (<I>Oryza sativa</I>) tonoplast monosaccharide transporters, <I>OsTMT1</I> and <I>OsTMT2</I>, we analysed their subcellular localization using green fluorescent protein (GFP) and expression patterns using reverse‐transcription polymerase chain reaction (RT‐PCR), performed histochemical β‐glucuronidase (GUS) assay and <I>in situ</I> hybridization analysis, and assessed sugar transport ability using isolated vacuoles.</P><P>Expression of OsTMT–GFP fusion protein in rice and Arabidopsis revealed that the OsTMTs localize at the tonoplast. Analyses of <I>OsTMT</I> promoter‐<I>GUS</I> transgenic rice indicated that <I>OsTMT1</I> and <I>OsTMT2</I> are highly expressed in bundle sheath cells, and in vascular parenchyma and companion cells in leaves, respectively. Both genes were found to be preferentially expressed in the vascular tissues of roots, the palea/lemma of spikelets, and in the main vascular tissues and nucellar projections on the dorsal side of the seed coats. Glucose uptake studies using vacuoles isolated from transgenic mutant Arabidopsis (<I>tmt1‐2‐3</I>) expressing <I>OsTMT1</I> demonstrated that OsTMTs are capable of transporting glucose into vacuoles.</P><P>Based on expression analysis and functional characterization, our present findings suggest that the <I>OsTMTs</I> play a role in vacuolar glucose storage in rice.</P></P>
( Dae Young Cheung ),( Hyung Keun Kim ),( Young Seok Cho ),( Sung Soo Kim ),( Jin Il Kim ),( Soo Heon Park ),( Hiun Suk Chae ),( Jae Kwang Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background and aim: Small rectal neuroendocrine tumors (NETs) can be treated with endoscopic resection. Endoscopic submucosal dissection (ESD) has been accepted as a reliable technique, but very diffi cult to do. This study aimed to evaluate the feasibility and effi cacy of precut and endoscopic mucosal resection (CSI-EMR) for rectal NETs comparing to ESD. Materials and Methods: From January 2011 to March 2013, patients with rectal NETs were enrolled consecutively. ESD or CSI-EMR was performed on operator`s discretion. Histological and clinical outcomes were measured and compared between the two treatment modalities. Results: A total of 33 patients were enrolled during the study periods. Seventeen NETs were treated in ESD method and 16 were in CSI-EMR. Both groups had similar mean tumor diameters (ESD 7.53 ± 1.94 vs. CSI-EMR 6.63 ± 1.99 mm; p = 0.197). En bloc resection was achieved in 100% of ESD group and 87.5% of CSI-EMR group. Lateral margin involvement occurred in 1 patient in ESD group and 2 in CSI-EMR group. Histologically complete resection rate was 88.2% (15 of 17) in the ESD group and 81.2% (13 of 16) in CSI-EMR group (p = 0.592). One case of perforation occurred in both group. Delayed bleeding did not happen. All measured outcomes above were not different between the two groups. Operation time was signifi cant shorter in CSIEMR group than in ESD group, 9.69 minutes and 20.12 minutes respectively (p-value = 0.004). Conclusion: CSI-EMR results in reliable clinical outcomes for small rectal NETs comparable to that of ESD. The CSI-EMR is technically feasible and more time saving.