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- 저자 : Chi-Hsin Chiang (검색결과 2 건)
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Chin-Cheng Lin,Pin-Chi Tang,Hsin-I Chiang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.6
Incidents of food fraud have occurred worldwide,particularly in the form of meat adulteration. In thisstudy, molecular probes were developed using the Randomamplification of polymorphic DNA (RAPD) polymerasechain reaction (PCR) technique in order to identify threebeef subspecies–Holstein, Angus, and Taiwan YellowCattle. Four RAPD-PCR 10-nucleotide primers were chosenout of a total of 60 primers. The selection was based onthe reproducibility of species-specific amplicons able todetect various origins of cattle breeds. The resultsdemonstrated that primer OPK12 produced three uniqueamplicons (1100 bp, 1000 bp and 480 bp) in Holstein;primer OPK14 generated one amplicon that only appearedin Holstein and Angus (200 bp); primer OPK19 amplifiedtwo species-specific amplicons in Holstein measuring550 bp and 650 bp, respectively. However, due to therelatively lower repeatability of RAPD-PCR, higher andmore specific testing repeats were required to increase theaccuracy of the conclusion.
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Hung-Yen Chin,Chin-Jung Wang,Eileen Changchien,Mei-Fung Lin,Chi-Hsin Chiang 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.4
Purpose: The aim of this study is to explore non-steroid anti-inflammation drugs (NSAIDs) potency for pelvic floor muscle pain by measuring local concentration in a rat model. Materials and Methods: We used nine NSAIDs, including nabumetone,naproxen, ibuprofen, meloxicam, piroxicam, diclofenac potassium, etodolac, indomethacin, and sulindac, and 9 groups of female Wister rats. Each group of rats was fed with one kind of NSAID (2 mg/mL) for three consecutive days. Thereafter, one mL of blood and one gram of pelvic floor muscle were taken to measure drug pharmacokinetics, including partition coefficient, lipophilicity, elimination of half-life (T1/2) and muscle/plasma converting ratio (Css, muscle/Css, plasma). Results: Diclofenacpotassium had the lowest T1/2 and the highest mean Css, muscle/Css, plasma (1.9 hours and 0.85±0.53, respectively). The mean Css, muscle/Css, plasma of sulindac, naproxen and ibuprofen were lower than other experimental NSAIDs. Conclusion: Diclofenac potassium had the highest disposition in pelvic floor muscle in a rat model. The finding implies that diclofenac potassium might be the choice for pain relief in pelvicmuscle.
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