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Colorimetric SNP Genotyping Based on Allele‐Specific PCR by Using a Thiol‐Labeled Primer
Jung, Ye Lim,Jung, Cheulhee,Parab, Harshala,Cho, Dae‐,Yeon,Park, Hyun Gyu WILEY‐VCH Verlag 2011 Chembiochem Vol.12 No.9
<P><B>Red gold/blue gold:</B> A highly convenient colorimetric method for the identification of single‐nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele‐specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the <I>BRCA1</I> gene.</P>
Jung, Ye Lim,Jung, Cheulhee,Park, Jung Hun,Kim, Moon Il,Park, Hyun Gyu The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.23
<P>A label-free, colorimetric method has been developed for ultrasensitive detection of nucleic acids that is based on photoinduced silver ion (Ag<SUP>+</SUP>) reduction around DNA bases. The assay system is capable of directly detecting bacterial genomic DNA without the need for PCR amplification.</P> <P>Graphic Abstract</P><P>A label-free, colorimetric method has been developed for ultrasensitive detection of nucleic acids that is based on photoinduced silver ion (Ag<SUP>+</SUP>) reduction around DNA bases. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc38552c'> </P>
A stochastic DNA walker that traverses a microparticle surface
Cheulhee JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Molecular machines have previously been designed that are propelled by DNAzymes, protein enzymes and strand displacement. These engineered machines typically move along precisely defined one- and two-dimensional tracks. Here, we report two different DNA walker systems that use hybridization to drive walking on DNA-coated microparticle surfaces. Through purely DNA:DNA hybridization reactions, the nanoscale movements of the walker can lead to the generation of a single-stranded product and the subsequent immobilization of fluorescent labels on the microparticle surface. The first walker (bipedal) can take more than 30 continuous steps and then it is released from the microparticle surface. The second walker (unipedal) is designed to stay persistently bound to the particle surface through multiple turnovers, and can take at least 47 (and likely more) steps without release. The third walker (multipedal) have four legs and its movement is controlled by alternating pH changes. These autonomous walkers may be of use in analytical, diagnostic applications and practical nanomachines.
Photopatterned Polydiacetylene Images Using a DNA Bio-Photomask
Jung, Yun Kyung,Jung, Cheulhee,Park, Hyun Gyu American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.24
<P>We describe a novel strategy to fabricate a well-defined polydiacetylene (PDA) supramolecular pattern on polyvinylidene fluoride (PVDF) membrane utilizing a DNA bio-photomask. By focusing on that, the absorption spectrum of DNA molecules having a lambda(max) at 260 nm overlaps with the wavelength at which the photopolymerization of the diacetylene monomer occurs, DNA molecules are used to define specific patterns on PVDF membranes coated with diacetylene lipids by shielding the applied 254 nm UV light and consequently preventing the photopolymerization of the lipids. As a result, the DNA-covered regions retain the original white color on the membrane while the regions uncovered by DNA molecules undergo the color change to blue through the photopolymerization by 254 nm UV irradiation. On the basis of the selective illumination through a DNA photomask, we precisely manufacture specific patterns using a microarray spotting method and also demonstrate the capability of this strategy to achieve a novel colorimetric DNA sensor without any complicated process.</P>
Jung, Cheulhee,Mun, Hyo Young,Li, Taihua,Park, Hyun Gyu IOP Pub 2009 Nanotechnology Vol.20 No.3
<P>A simple, highly efficient immobilization method to fabricate DNA microarrays, that utilizes gold nanoparticles as the mediator, has been developed. The fabrication method begins with electrostatic attachment of amine-modified DNA to gold nanoparticles. The resulting gold–DNA complexes are immobilized on conventional amine or aldehyde functionalized glass slides. By employing gold nanoparticles as the immobilization mediator, implementation of this procedure yields highly homogeneous microarrays that have higher binding capacities than those produced by conventional methods. This outcome is due to the increased three-dimensional immobilization surface provided by the gold nanoparticles as well as the intrinsic effects of gold on emission properties. This novel immobilization strategy gives microarrays that produce more intense hybridization signals for the complementary DNA. Furthermore, the silver enhancement technique, made possible only in the case of immobilized gold nanoparticles on the microarrays, enables simple monitoring of the integrity of the immobilized DNA probe. </P>