Transcriptome and proteomics-based analysis to investigate the regulatory mechanism of silk gland differences between reciprocal cross silkworm, Bombyx mori

Ge Qi,Xiao Rui,Yuan Yi,He Suqun,Chen Liang,Ma Shangshang,Taha Rehab Hosny,Yao Qin,Chen Keping 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

The silkworm, Bombyx mori, is an important model economic insect. Cross breeding is an important way to select excellent silkworm varieties, and different combinations among the same parents often show different genetic characters. To explore the regulatory mechanism of silk gland differences in reciprocal cross silkworm, the 3rd day of the 5th instar of the orthogonal silkworm (306♀ × NB♂, 798♀ × NB♂) and the reverse cross silkworm (NB♀ × 306♂, NB♀ × 798♂) silk glands were used as experimental materials, then using Label-free-based proteomics and RNA-Seq-based transcriptomics for analysis. Besides, the qRT-PCR was used to validate the expression of differentially expressed genes. The results showed that in NB♀ × 306♂ VS 306♀ × NB♂ group, 280 differentially expressed genes and 238 differential proteins were up-regulated while 40 differentially expressed genes and 149 differential proteins were down-regulated; in NB♀ × 798♂ VS 798♀ × NB♂, 82 differentially expressed genes and 325 differential proteins were up-regulated while 113 differentially expressed genes and 258 differential proteins were down-regulated. Further analysis indicated that the up-regulation of mitochondrial aldehyde dehydrogenase gene and cytochrome P450 gene, which are related to mitochondria, might be regulated by maternal inheritance. The GO and KEGG enrichment initially showed that the differential genes and proteins are mainly involved in processes such as protease metabolism, ATP hydrolysis, lipid transport, insect hormone synthesis, and TCA cycle. The down-regulated expression of ecdysone oxidase and Serpin-32 in the reverse cross group will reduce the energy utilization in the process of silk gland synthesis, increase the hydrolysis of serine, affect the synthesis and transport of silk protein, and cause the difference between reciprocal crosses.

SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway

Chen, Liang-Liang,Gao, Ge-Xin,Shen, Fei-Xia,Chen, Xiong,Gong, Xiao-Hua,Wu, Wen-Jun Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.9

As the most common type of endocrine malignancy, papillary thyroid cancer (PTC) accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the $Wnt/{\beta}-catenin$ signaling pathway in human PTC cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, $Wnt/{\beta}-catenin$ signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finally, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated $Wnt/{\beta}-catenin$ signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the $Wnt/{\beta}-catenin$ signaling pathway. Besides, $si-{\beta}-catenin$ was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the $Wnt/{\beta}-catenin$ signaling pathway in human PTC.

Serum miR-19a Predicts Resistance to FOLFOX Chemotherapy in Advanced Colorectal Cancer Cases

Chen, Qi,Xia, Hong-Wei,Ge, Xiao-Jun,Zhang, Yu-Chen,Tang, Qiu-Lin,Bi, Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Colorectal cancer is the fourth most common cancer worldwide and the second leading cause of cancer-related death. FOLFOX is the most common regimen used in the first-line chemotherapy in advanced colorectal cancer, but only half of the patients respond to this regimen and we have almost no clue in predicting resistance in such first-line application. Methods: To explore the potential molecular biomarkers predicting the resistance of FOLFOX regimen as the first-line treatment in advanced colorectal cancer, we screened microRNAs in serum samples from drug-responsive and drug-resistant patients by microarrays. Then differential microRNA expression was further validated in an independent population by reverse transcription and quantitative real-time PCR. Results: 62 microRNAs expressing differentially with fold-change >2 were screened out by microarray analysis. Among them, 5 (miR-221, miR-222, miR-122, miR-19a, miR-144) were chosen for further validation in an independent population (N=72). Our results indicated serum miR-19a to be significantly up-regulated in resistance-phase serum (p=0.009). The ROC curve analysis showed that the sensitivity of serum miR-19a to discriminate the resistant patients from the response ones was 66.7%, and the specificity was 63.9% when the AUC was 0.679. We additionally observed serum miR-19a had a complementary value for cancer embryonic antigen (CEA). Stratified analysis further revealed that serum miR-19a predicted both intrinsic and acquired drug resistance. Conclusions: Our findings confirmed aberrant expression of serum miR-19a in FOLFOX chemotherapy resistance patients, suggesting serum miR-19a could be a potential molecular biomarker for predicting and monitoring resistance to first-line FOLFOX chemotherapy regimens in advanced colorectal cancer patients.

어린이 체험 강화를 위한 어린이 박물관 전시 공간 디자인 연구

진고은(Chen,Ge-Ning),남경숙(Nam,Kyung-Sook) 한국실내디자인학회 2018 한국실내디자인학회 학술대회논문집 Vol.20 No.3

This research to strengthen children"s tourist experience, the museum for children in the museum display space research.To choose the contents, show the development of children"s body and mind health infectious art exhibition, create a highly of the use of reasonable and conform to the children"s aesthetic characteristics the independence of the exhibition, such a display space and children"s psychological development, cognitive level of the consciousness of the self promotion and development consistent with and, at the same time, pay attention to the status of children, give full play to their initiative, and environmental atmosphere to better understand the world of easy pleasure in helping children.

東西方倫理文化差異比較硏究

갈신홍 ( Chen Hong Ge ),리태건 ( Tae Ken Rhee ),최익만 ( Ik Man Choi ) 한국외국어대학교 중국연구소 2010 中國硏究 Vol.50 No.-

Many famous classical writers think that the East and the West has formed two different ways when commenting on the historical process from primitive society to enter the civilized society. These two different ways are represented by Ancient Greece of "Classical Ancient", and represented by ancient Eastern countries of "Ancient Asia". Specifically, the development path of "Classical Ancient" is from clan to private property and then to state, individual private ownership breaks through the clan organization, state replaces the clan. In the absence of digestion of the original clan organization, the "Ancient Asia" directly develops into slavery countries, and consanguinity clan system integrates with national organization form. Different paths of historical development have created different cultural characteristic, therefore there are many differences between Eastern and Western culture. "Cultural Comparison" is a major problem, limited to textual length, in this article we will mainly do some research on different rational thinking mode, social governance mode, human nature theory and other issues of Eastern and Western ethical culture, and study the social causes behind cultural differences. For example, while Western culture highlights the cultural system of scientific rationality, Eastern culture focuses on the thinking system of value rationality; Eastern social governance follows a path different from the Western contract legal system; in the theory of human nature, the Western advocates of evil of human nature, the Eastern of Confucian advocates of good of human nature. Therefore, the result is "politics of spread the goodness" in Western in comparison with "politics of restrain the evil" in Eastern of Confucian. The existence of every culture has its realistic reasons and historical reasons for its existence. Investigating the significant differences between Eastern and Western culture and studying the deep reasons of these differences has important significance for understanding the differences between Eastern and Western culture, also better understanding Chinese culture, Korean culture, Western culture, accommodating Eastern and Western Culture to get better development of human culture. The social and material condition of production determines the mode of social life, and also the ideology and culture of the mode of social life. If we want to understand the history and ideology culture of East and West, and if we want to understand the Confucianism which guides and influences the history and culture of Eastern, we must make deep research on background of the social and historical causes of the Confucianism of East to analyze the specific social and historical conditions. From the view of different ways which generate the Eastern and Western history road, "Classical Ancient" is formed on the basis of private ownership after the collapse of the system "Ancient Asia" is transformed from the clan public ownership of land into state ownership of land. Direct transition from the clan to the state makes the whole social structure of generating legacy system of clan, which is linked by consanguinity. This is the key issue to understand the mode of production, social system, ideology and culture of the ancient Orient, and especially it is the direct historical basis to study the formation and characteristics of Confucianism. The history road of "Asian" shows that public ownership of land in ancient orient and the forms of social organization that determined by low productivity, makes the national publics function created in the absence of complete differentiation and disintegration of the clan organization. Thus, the country`s social structure naturally depends on the natural consanguineous organization. "Classical Ancient" is from the family to private property and then to the state. The family is replaced by state. "Ancient Asia" is from family to the state and the state is mixed with the family. Clan exists as prototype organization for the nation. The state is established by the relationship of clan consanguinity, the rule mode, which can be learned from and imitated, is also directly derived from the clan rule mode. The strong legacy system of clan in state`s political system makes the social structure, cultural spirit and historical process of ancient orient with great particularity. The Confucianism founded by Confucius is a theoretical reflection of real existence of clan (patriarchal clan system) of the Ancient Oriental state and another extension of the history road of "Asian" in the ideological field. Today, as a very profound way, globalization is gradually reconstructing the social life of different countries and nations all over the world. Due to the historical and practical reasons, the Eastern and Western has developed two different historical paths, creating their own characteristics objectively. How to grasp the demands of cultural accommodation in the world of globalization, how to get better development in the cultural conflict and cultural integration of these two different culture, it is the question which must be answered in the study of comparative subject of Eastern and Western culture.

Purification and Characterization of Novel Bifunctional Xylanase, Xyn3, Isolated from Aspergillus niger A-25

( Hong Ge Chen ),( Xin Yan ),( Xin Yu Liu ),( Ming Dao Wang ),( Hui Min Huang ),( Xin Cheng Jia ),( Jin An Wang ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.7

Effect of curing mechanism on sulfuric acid corrosion resistance of geopolymer recycled aggregate concrete

Zhenmeng Chen,Hui Liu,Pinghua Zhu,Haichao Li,Tianze Ge,Lei Yang,Chunhong Chen,Yanlong Dong 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4

To explore the effect of curing mechanism on the mechanical properties and sulfuric acid corrosion resistance of the geopolymer recycled aggregate concrete (GRAC), the specimens were cured at high temperature (60oC, 80oC) for 6 h, 24 h and 48 h respectively, and then lasted up to 7 d at room temperature. After the curing period was over, the laboratory accelerated simulation test of specimens was carried out by the periodic immersion method: the GRAC specimens (φ 50 × 100 mm) were immersed in the sulfuric acid solution with pH = 1 for 5 d and then dried at room temperature for 24 h, with a total circulation of 90 d. The results showed that after being eroded by sulfuric acid solution, the GRAC specimens cured at 60oC for 48 h had higher compressive strength with 19.9 MPa, lower mass loss rate (only 0.23%) and neutralization depth (5.71 mm) than those under other curing time. The GRAC specimens cured 80oC for 24 h also had a good performance: compressive strength achieved 16.9 MPa and had lower neutralization depth (5.99 mm), which was less affected by sulfuric acid corrosion. However, the GRAC specimens cured at 60oC for 48 h had the better mechanical properties than that cured at 80oC for 24 h. Excessive high temperature curing (80oC) would lead to more voids and microcracks inside GRAC, and destroy the dense structure, thereby reducing mechanical property of concrete. These voids and pores provided more erosion channels for sulfuric acid solution, which accelerated the corrosion rate. From the point of view of energy saving and concrete performance, no more than 60°C of curing temperature is recommended for industrial use.

從明末時期"祖師禪” 與“狂禪”之辨看中國禪法的晩景

( Yong Ge Chen ) 동아시아불교문화학회 2013 동아시아불교문화 Vol.0 No.16

Following with the of trend of Mind School which advocated by Wang yangming(王陽明) Since mid-Ming Dynasty, the relationship became more close together between Confucianism and Buddhism, which arose the trends of flourishing both Confucianism and Buddhism during late Ming Dynasty. However, this trend caused a lot of critics in Confucianist, particularly by Jishan school(葺山學派) which began with Liu zongzhou (劉宗周) & Huangzongxi (黃宗羲) in Zhejiang Province;and Dong-lin School (東林學派) which began with Gu xiancheng (顧憲成) and Gao panlong (高攀龍) etc. in Wuxi, Jiangsu Province. It is the main content of this paper that discussed the relationship between Confucianism & Buddhism and its influence of Confucianist thought, and discussion philosophical transformation on the relationship between "Kuang Chan(狂禪) "and "Zushi Chan(祖 師禪)" during late Ming Dynasty.

노인 외출편의를 위한 유니버설관점의 지하철공공시설 디자인 연구 - 지하철 진입시설이 종심으로 -

진고은(Chen,Ge-Ning),남경숙(Nam,Kyung-Sook) 한국실내디자인학회 2019 한국실내디자인학회 학술대회논문집 Vol.21 No.1

Depending on the development of the city, consideration for the elderly and special designs can be easily found in Gonggung space.The old are the important groups that our society constitutes, and reflect the level of development of their lives, whether they are good or bad, or a country, and are an important expression of people"s happiness in life. Urban subway facilities are an important part of urban public facilities and should consider the use of the underprivileged and special classes in addition to meeting basic functionality. Today"s design does not fully take into account the psychology of wheelchair-using seniors, and the unusual design is discriminated against and has a bad idea. True rational design should eliminate this discrimination and achieve equality. The theory of universal design was established through the literature research of prior research. Second, through empirical investigation, four or more transit lines out of the subway transit stations currently used in the market were analyzed and analyzed for problems and requirements of various users. Further, the above findings and behavioral characteristics of the elderly were observed and a checklist was established that applied universal design guidelines for the design of subway facilities.

≪국제학술대회: 중국헌법상 경제질서의 변모와 중국투자의 법적 과제≫ : 중국의 WTO가입과 중국대외무역법체제의 변화

이거천 ( Ge Chen Lee ) 국제헌법학회, 한국학회 2004 世界憲法硏究 Vol.10 No.-