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- 저자 : Chatti, Ines Bouhlel (검색결과 5 건)
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Chatti, Ines Bouhlel,Limem, Ilef,Boubaker, Jihed,Skandrani, Ines,Kilani, Soumaya,Bhouri, Wissem,Sghaier, Mohamed Ben,Nefatti, Aicha,Mansour, Hedi Ben,Ghedira, Kamel,Chekir-Ghedira, Leila The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3
The extract enriched in total oligomer flavonoids (TOF), and the aqueous, methanol, and ethyl acetate extracts of Acacia salicina were investigated for their polyphenolic compound content, antioxidative activity in the nitro blue tetrazolium/riboflavin assay system, antibacterial activity against Gram-positive and Gram-negative bacterial reference strains, antigenotoxic activity tested with the Ames assay, and cytotoxic activity against the K562 human chronic myelogenous leukemia cell line and L1210 leukemia murine cells. TOF extract was effective at inhibiting nitro blue tetrazolium reduction by superoxide radical in a nonenzymatic $O_2^{\cdot-}$-generating system. Significant activity against bacterial reference strains Staphylococcus aureus, Enterococcus faecalis, Salmonella enteritidis, and Salmonella typhimurium was shown with all the tested extracts. These extracts significantly decreased the genotoxicity induced by sodium azide and 4-nitro-o-phenylenediamine. A pronounced cytotoxic effect on both leukemia cell lines was detected in TOF, methanolic and ethyl acetate extracts. The antioxidant, antimicrobial, antigenotoxic, and cytotoxic activities exhibited by A. salicina depended on the chemical composition of the tested extracts.
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Ines Bouhlel Chatti,Ilef Limem,Jihed Boubaker,Ines Skandrani,Soumaya Kilani,Wissem Bhouri,Mohamed Ben Sghaier,Aicha Nefatti,Hedi Ben Mansour,Kamel Ghedira,Leila Chekir-Ghedira 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3
The extract enriched in total oligomer flavonoids (TOF), and the aqueous, methanol, and ethyl acetate extracts of Acacia salicina were investigated for their polyphenolic compound content, antioxidative activity in the nitro blue tetrazolium/riboflavin assay system, antibacterial activity against Gram-positive and Gram-negative bacterial reference strains, antigenotoxic activity tested with the Ames assay, and cytotoxic activity against the K562 human chronic myelogenous leukemia cell line and L1210 leukemia murine cells. TOF extract was effective at inhibiting nitro blue tetrazolium reduction by superoxide radical in a nonenzymatic O2•−-generating system. Significant activity against bacterial reference strains Staphylococcus aureus, Enterococcus faecalis, Salmonella enteritidis, and Salmonella typhimurium was shown with all the tested extracts. These extracts significantly decreased the genotoxicity induced by sodium azide and 4-nitro-o-phenylenediamine. A pronounced cytotoxic effect on both leukemia cell lines was detected in TOF, methanolic and ethyl acetate extracts. The antioxidant, antimicrobial, antigenotoxic, and cytotoxic activities exhibited by A. salicina depended on the chemical composition of the tested extracts.
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Ines Bouhlel Chatti,Imene Ben Toumia,Yosr Krichen,Mouna Maatouk,Leila Chekir Ghedira,Mounira Krifa 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.9
Rhamnus alaternus (Rhamnaceae) has been used as a laxative, purgative, diuretic, antihypertensive, and depurative. However, few scientific research studies on its antimelanoma activity have been reported. This study aimed to investigate the in vitro antimelanoma effect of an enriched total oligomer flavonoid (TOF) extract, from R. alaternus, and to identify its phytochemical compounds. The chemical composition of TOF extract was assessed by HPLC-electrospray ionization tandem mass spectrometry (HPLC/ESI-MS2) analysis. Antimelanoma activity was determined on cultured tumor cell B16F10 by the crystal violet assay, the alkaline comet assay, acridine orange/ethidium bromide (AO/EB), annexin V-fluorescein isothiocyanate/ propidium iodide (V-FITC/PI) staining, the cell cycle distribution, and the wound healing assay. Regarding chemical composition, a mixture of quercetin diglucoside, quercetin-3-O-neohesperidoside, kaempferol-3-O-(2G-α-L-rhamnosyl)-rutinoside, rhamnetin hexoside, kaempferol-3-O-rutinoside, rhamnocitrin hexoside, pilosin hexoside, apigenin glucoside, and kaempferol-3-O-glucoside was identified as major phytochemical compounds of the extracts. TOF extract inhibits melanoma B16F10 cell proliferation in dose-dependent manner. The induction of apoptosis was confirmed by comet assay, AO/EB, and annexin V-FITC/PI test. TOF extract could also induce S phase cell cycle, inhibit, and delay the cell migration of B16F10 cells. The findings showed that TOF extract from R. alaternus could be a potentially good candidate for future use in alternative antimelanoma treatments.
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Generic Framework for Hybrid Fault Diagnosis and Health Monitoring of the Tennessee Eastman Process
Khaoula Tidriri,Nizar Chatti,Sylvain Verron,Teodor Tiplica 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
Fault Diagnosis and Health Monitoring (FD-HM) based on hybrid approaches have been an active field of research and a key challenge over the last few years. In many applications, generic and unified approaches are usually required for designing a complete robust FD-HM system. The main contribution of this article is to develop a hybrid approach properly tailored for such challenge, by bridging the Bond-Graph (BG), the Signed Bond-Graph (SBG) and the Bayesian Network (BN) methods, through a probabilistic common framework for decision-making. This new hybrid methodology benefits from different types of information emanating from the quantitative model, the qualitative reasoning and the available data, in order to increase the overall confidence in the diagnosis performances. The effectiveness of the proposed hybrid approach is validated by the well-known Tennessee Eastman Process.
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Amani Cheikh,Rym Benkhalifa,Zied Landoulsi,Imen Chatti,Mohamed El Ayeb 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.11
AahG50, the toxic fraction of Androctonus australishector venom, was studied on human Kv3.1 channelsactivation, stably expressed in Xenopus oocytes using the twoelectrodevoltage clamp technique. AahG50 reduced Kv3.1currents in a reversible concentration-dependent manner,withan IC50 value and a Hill coefficient of 40.4 ± 0.2 lg/ml and1.3 ± 0.05, respectively. AahG50 inhibited IKv3.1 withoutmodifying the current activation kinetics. The AahG50-induced inhibition of Kv3.1 channels was voltage-dependent,with a gradual increase at lower concentrations and over thevoltage range of channels opening. However, at higher concentrations,the inhibition exhibited voltage dependence onlyin the first range of channels opening from -20 to ?10 mV,but demonstrates a low degree of voltage-dependence whenchannels are fully activated. In the literature, toxins havepreviously been isolated from AahG50, KAaH1 and KAaH2and were reported not to have any effect on IKv3.1. Thepresent article’s findings suggest that AahG50 may contain apeptidic component active on Kv3.1 channels, which inhibitsIKv3.1 in a selective manner.
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