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Gu, Chao-Jiang,Zheng, Cong-Yi,Zhang, Qian,Shi, Li-Li,Li, Yong,Qu, San-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.1
To prove whether error catastrophe /lethal mutagenesis is the primary antiviral mechanism of action of ribavirin against foot-and-mouth disease virus (FMDV). Ribavirin passage experiments were performed and supernatants of $Rp_1$ to $Rp_5$ were harvested. Morphological alterations as well as the levels of viral RNAs, proteins, and infectious particles in the BHK-21 cells infected using the supernatants of $Rp_1$ to $Rp_5$ and control were measured by microscope, real-time RT-PCR, western-blotting and plaque assays, respectively. The mutation frequency was measured by sequencing the complete P1- and 3D-encoding region of FMDV after a single round of virus infection from ribavirin-treated or untreated FMDV-infected cells. Ribavirin treatment for FMDV caused dramatically inhibition of multiplication in cell cultures. The levels of viral RNAs, proteins, and infectious particles in the BHK-21 cells infected were more greatly reduced along with the passage from $Rp_1$ to $Rp_5$, moreover, nucleocapsid protein could not be detected and no recovery of infectious virus in the supernatant or detection of intracellular viral RNA was observed at the $Rp_5$-infected cells. A high mutation rate, giving rise to an 8-and 11-fold increase in mutagenesis and resulting in some amino acid substitutions, was found in viral RNA synthesized at a single round of virus infection in the presence of ribavirin of $1000\;{\mu}M$ and caused a 99.7% loss in viral infectivity in contrast with parallel untreated control virus. These results suggest that the antiviral molecular mechanism of ribavirin is based on the lethal mutagenesis/error catastrophe, that is, the ribavirin is not merely an antiviral reagent but also an effective mutagen.
Design and Implementation of Transfusion Auxiliary Device by Patient Manual Control Dressing Change
Jiang Jin-gang,Shen Rui-chao,Wang Bao-fu,Gu Bo-yang,Tang Hai-bo,Jiang Ze-hao 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.11
Transfusion auxiliary device by patient manual control dressing is proposed. It is realized by one-way rotation ratchet mechanism, which is consistent of slider-crank mechanism and double pawl ratchet mechanism. Dressing change is automatically realized by patient manual control rope. This reduces the labor intensity of the medical staff, shortens the waiting time of the patients. This device has the advantage of low cost, large market space.
A Weakly Cationic Temperature Tolerant and Salt Resistant Polymer: Synthesis and Properties
Bo Deng,Xueqin Luo,Feng Jiang,Wei Liu,Jianwei Gu,Chao Liu,Yanan Song 한국고분자학회 2022 Macromolecular Research Vol.30 No.8
In the petroleum industry, water-soluble polymers can be used as oil displacement agents. However, the use of water-soluble polymers is limited because of poor temperature and salt resistance. To improve temperature and salt resistance, a weakly cationic polymer with large side groups (PAM/AMPS/VI) was prepared by copolymerizing acrylamide (AM) with 2-acrylamido-2-methylpropane sulfonic acid (AMPS) and 1-vinylimidazole (VI). The viscosity of PAM/AMPS/VI water solutions can be increased more than 20 mPa·s compared with PAM/AMPS. In addition, the viscosity of the solution increased continuously after aging at 80℃, showing good temperature and salt stability. The protonated tertiary amine in the imidazole ring electrostatically interacts with the sulfonic group, increasing the viscosity and salt resistance of the polymer. The five-membered ring of imidazole also enhances the rigidity of the polymer chain and improves the temperature tolerance. As a confirm of the result, a complete spatial network of PAM/ AMPS/VI was observed in scanning electron microscopy (SEM) micrographs. Using weak cationic polymers with large side groups can provide a reference for the design of new temperature tolerance and salt resistant polymer.