Trueness Assessment for Serum Glucose Measurement Using Commercial Systems through the Preparation of Commutable Reference Materials

ChangYu Xia,Ou Liu,LanZhen Wang,GuoBing Xu 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.4

Background: Commutable reference materials (RMs) are suitable for end-users for evaluating the metrological traceability of values obtained using routine measurement systems. We assessed the performance of 6 routine measurement systems with validated secondary RMs. Methods: We tested the homogeneity, stability, and commutability of 5 minimally processed human serum pools according to the standard guidelines. The serum pools were assigned values as per the reference procedure of the United States Centers for Disease Control and were used to evaluate the trueness of results from 6 commercial measurement systems based on enzymatic methods: 3 glucose oxidase (GOD) and 3 hexokinase (HK) methods. Results: The prepared RMs were validated to be sufficiently homogenous, stable, and commutable with the patient samples. Method bias varied for different systems: GOD01, -0.17 to 2.88%; GOD02, 1.66 to 4.58%; GOD03, -0.17 to 3.14%; HK01, -3.48 to -0.85%; HK02, -3.83 to -0.11%, and HK03, -1.82 to -0.27%. Conclusions: We observed that the prepared serum glucose RMs were qualified for trueness assessment. Most of the measurement systems met the minimal quality specifications. Background: Commutable reference materials (RMs) are suitable for end-users for evaluating the metrological traceability of values obtained using routine measurement systems. We assessed the performance of 6 routine measurement systems with validated secondary RMs. Methods: We tested the homogeneity, stability, and commutability of 5 minimally processed human serum pools according to the standard guidelines. The serum pools were assigned values as per the reference procedure of the United States Centers for Disease Control and were used to evaluate the trueness of results from 6 commercial measurement systems based on enzymatic methods: 3 glucose oxidase (GOD) and 3 hexokinase (HK) methods. Results: The prepared RMs were validated to be sufficiently homogenous, stable, and commutable with the patient samples. Method bias varied for different systems: GOD01, -0.17 to 2.88%; GOD02, 1.66 to 4.58%; GOD03, -0.17 to 3.14%; HK01, -3.48 to -0.85%; HK02, -3.83 to -0.11%, and HK03, -1.82 to -0.27%. Conclusions: We observed that the prepared serum glucose RMs were qualified for trueness assessment. Most of the measurement systems met the minimal quality specifications.

Identification and prevention of heterotopias in mouse neocortical neural cell migration incurred by surgical damages during utero electroporation procedures

Bolin Wang,Liting Ji,카우식 비샤이,Changyu Li,허성오 한국통합생물학회 2020 Animal cells and systems Vol.24 No.2

In utero electroporation (IUE) is a useful technique for gene delivery in embryonic mouse brain. IUE technique is used to investigate the mammalian brain development in vivo. However, according to recent studies, IUE methodology has some limitations like the formation of artificial ectopias and heterotopias at the micro-injection site. Thus far, the artificial heterotopias generated by physical trauma during IUE are rarely reported. Here, we reported the artificial heterotopias and ectopias generated from surgical damages of micropipette in detail, and moreover, we described the protocol to avoid these phenotypes. For the experimental purpose, we transferred empty plasmids (pCAGIG-GFP) with green fluorescent-labelled protein into the cortical cortex by IUE and then compared the structure of the cortex region between the injected and un-injected cerebral hemispheres. The coronary section showed that ectopias and heterotopias were appeared on imperfect-injected brains, and layer maker staining, which including Ctip2 and TBR1 and laminin, can differentiate the physical damage, revealing the neurons in artificial ectopic and heterotopic area were not properly arranged. Moreover, premature differentiation of neurons in ectopias and heterotopias were observed. To avoid heterotopias and ectopias, we carefully manipulated the method of IUE application. Thus, this study might be helpful for the in utero electroporator to distinguish the artificial ectopias and heterotopias that caused by the physical injury by microneedle and the ways to avoid those undesirable circumstances.

Value functions and error bounds of trust region methods

Wenling Zhao,Changyu Wang 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.24 No.1

VALUE FUNCTIONS AND ERROR BOUNDS OF TRUST REGION METHODS

Zhao, Wenling,Wang, Changyu 한국전산응용수학회 2007 Journal of applied mathematics & informatics Vol.24 No.1

This paper studies some properties of the value functions and gives some sufficient and necessary conditions about the presented global error and local error. And it leads to one kind of relationship between iterative points and optimal solution or K-T point.

LQR Pendulation Reduction Control of Ship-Mounted Crane Based on Improved Grey Wolf Optimization Algorithm

Mingxiao Sun,Changyu Ji,Tiantian Luan,Nan Wang 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.3

The poor adaptability matrix of traditional LQR controller causes the problems of large payload swing and slow response for ship-mounted cranes during operation. To solve such problems, an LQR controller based on an improved grey wolf optimization algorithm (IGWO-LQR) is proposed. Firstly, the dynamics model of ship-mounted crane is constructed, the pendulum reduction problem is transformed into the LQR quadratic performance index problem, and IGWO is used to optimize the weight matrix. At the same time, the RBF neural network is applied to compensate for the non-linear wave disturbances in the system. Finally, the pendulum reduction efficiency of the controller under different parameters and conditions is verified by numerical simulation. Compared with the traditional LQR controller, the simulation results show that the control accuracy of the IGWO-LQR controller is improved by about 5%, and the response speed is improved by about 5–10 s. This method can significantly reduce the payload swing and improve work efficiency.

Combining trust region and linesearch algorithm for equality constrained optimization

Zhensheng Yu,Changyu Wang,Jiguo Yu 한국전산응용수학회 2004 Journal of applied mathematics & informatics Vol.14 No.-

In this paper, a combining trust region and line search algorithm for equality constrained optimization is proposed. At each iteration, we only need to solve the trust region subproblem once, when the trust region trial step can not be accepted, we switch to line search to obtain the next iteration. Hence, the difficulty of repeated solving trust region subproblem in an iterate is avoided. In order to allow the direction of negative curvature, we add second correction step in trust region step and employ nonmonotone technique in line search. The global convergence and local superlinearly rate are established under certain assumptions. Some numerical examples are given to illustrate the efficiency of the proposed algorithm.

Research on the Formation Mechanism of Shear Zone in the Neogene Mudstone Landslide

( Xin Peng ),( Wang Tao ),( Liu Jiamei ),( Liang Changyu ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The shear zone is the main control factor for the translational and rotational landslides.Different geometric forms of landslides were selected to analyze the process of structural change and the mechanical mechanisms of the bedding-parallel shear zone in Neogen period mudstones. The results show that there are weak layers, with high clay content, in translational landslides and rotational-translational landslides, such as those with bedding-parallel shear zone, while rotational landslides are composed of homogeneous material. The bedding-parallel shear zone can be divided into two parts: the main shear zone and the secondary shear zone. The main shear zone has an S-C fabric, the C plane has a strong scratch, and the S plane develops in high plastic mudstones. The clay contents of the main shear zone in a bedding-parallel sliding zone are greater than 50%, while the absolute content of minerals mixed with illite and montmorillonite exceeds to 20%. Creep and rapid slip are the main forms of structural deformation of the shear zone. Creep deformation mainly develops in the sliding zone with high amounts of clay particles; the microstructural adjustment of the slip zone shows no crack expansion and the clay minerals are a viscous flow. Progressive creep induced by water pressure and gravity will cause the acceleration of the creep and rapid sliding. Rapid sliding deformation, which is due to repeated shear, occurs along the fixed shear fracture-surface.

COMBINING TRUST REGION AND LINESEARCH ALGORITHM FOR EQUALITY CONSTRAINED OPTIMIZATION

Yu, Zhensheng,Wang, Changyu,Yu, Jiguo 한국전산응용수학회 2004 Journal of applied mathematics & informatics Vol.14 No.1

In this paper, a combining trust region and line search algorithm for equality constrained optimization is proposed. At each iteration, we only need to solve the trust region subproblem once, when the trust region trial step can not be accepted, we switch to line search to obtain the next iteration. Hence, the difficulty of repeated solving trust region subproblem in an iterate is avoided. In order to allow the direction of negative curvature, we add second correction step in trust region step and employ nonmonotone technique in line search. The global convergence and local superlinearly rate are established under certain assumptions. Some numerical examples are given to illustrate the efficiency of the proposed algorithm.

Solving a Class of Generalized Semi-Infinite Programming via Augmented Lagrangians

Haiyan Zhang,Fang Liu,Changyu Wang 한국전산응용수학회 2009 Journal of applied mathematics & informatics Vol.27 No.1

Under certain conditions, we use augmented Lagrangians to transform a class of generalized semi-infinite min-max problems into common semi-infinite min-max problems, with the same set of local and global solutions. We give two conditions for the transformation. One is a necessary and sufficient condition, the other is a sufficient condition which can be verified easily in practice. From the transformation, we obtain a new first-order optimality condition for this class of generalized semi-infinite min-max problems. Under certain conditions, we use augmented Lagrangians to transform a class of generalized semi-infinite min-max problems into common semi-infinite min-max problems, with the same set of local and global solutions. We give two conditions for the transformation. One is a necessary and sufficient condition, the other is a sufficient condition which can be verified easily in practice. From the transformation, we obtain a new first-order optimality condition for this class of generalized semi-infinite min-max problems.

SOLVING A CLASS OF GENERALIZED SEMI-INFINITE PROGRAMMING VIA AUGMENTED LAGRANGIANS

Zhang, Haiyan,Liu, Fang,Wang, Changyu The Korean Society for Computational and Applied M 2009 Journal of applied mathematics & informatics Vol.27 No.1

Under certain conditions, we use augmented Lagrangians to transform a class of generalized semi-infinite min-max problems into common semi-infinite min-max problems, with the same set of local and global solutions. We give two conditions for the transformation. One is a necessary and sufficient condition, the other is a sufficient condition which can be verified easily in practice. From the transformation, we obtain a new first-order optimality condition for this class of generalized semi-infinite min-max problems.