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Lima, Felipe Teixeira,Seba, Viviane,Silva, Gabriel,Torrezan, Guilherme Silva,Polaquini, Carlos Roberto,Pinhanelli, Vitor Caressato,Baek, Seung J.,Fachin, Ana Lú,cia,Regasini, Luis Octavio,Marins MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.7
<P>Curcumin is a potential anticancer drug with poor bioavailability, which limits its clinical use as a therapeutic agent. The aim of this study was a preliminary evaluation of the curcumin analogue CH-5 as a cytotoxic agent in human osteosarcoma cell lines U2OS, MG-63, and Saos-2. CH-5 inhibited cell viability at lower concentrations than curcumin, leading to the induction of apoptosis. The cellular levels of the transcription factors p53 and Sp1 affect the expression of cellular pathways that lead to apoptosis. CH-5 increased p53 protein levels in U2OS cells and reduced Sp1 levels, with a consequent effect on the expression of their target genes DNA methyltransferase 1 (<I>DNMT1)</I> and growth arrest and DNA damage-inducible 45 alpha gene (<I>Gadd45a)</I>. CH-5 repressed <I>DNMT1</I> and increased <I>Gadd45a</I> mRNA expression, which was dependent on p53, as this effect was only observed in the colorectal cancer cell line HCT116 with active p53, but not in the isogenic p53-deficient HCT116 cells. CH-5 also reduced the protein levels of DNMT1, which led to the upregulation of <I>Gadd45a</I>. These results suggest that CH-5 has potentially higher anticancer activity than curcumin, which is associated with the expression of apoptosis-associated genes regulated by the transcription factors Sp1 and p53. Future work on CH-5 will define the therapeutic potential of this compound in vivo.</P>
pep27 and LytA in Vancomycin-Tolerant Pneumococci
( Alma Olivares ),( Jose Olivares Trejo ),( Jose Arellano Galindo ),( Gerardo Zuniga ),( Gerardo Escalona ),( Juan Carlos Vigueras ),( Paula Marin ),( Juan Xicohtencatl ),( Pedro Valencia ),( Norma Ve 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.12
Vancomycin therapy failure due to the emergence of tolerance in pneumococci is increasing. The molecular mechanism of tolerance is not clear, but lytA and pep27 are known to be involved. Our aim was to evaluate the expression of both genes in vancomycin-tolerant Streptococcus pneumoniae (VTSP) strains. Eleven VTSP strains from a total of 309 clinical isolates of S. pneumoniae from 1997 to 2006 were classified according to the criteria of Liu and Tomasz. All VTSP strains were evaluated for susceptibility according to CLSI criteria, serotype by the Quellung test, and clonality by PFGE. The expressions of lytA and pep27 were analyzed in different growth phases by RT-PCR with and without vancomycin. Eighty-two percent of VTSP strains showed resistance to penicillin, and 100% were sensitive to vancomycin and cefotaxime. The most frequent serotypes of VTSP strains were 23F (4/11) and 6B (3/11). Clonal relationship was observed in only two strains. No significant changes were observed in pep27 expression in the three phases of growth in VTSP strains with and without vancomycin. Interestingly, pep27 expression in the stationary phase in the non-tolerant reference strain R6 was significantly higher. However, no significant differences in lytA expression were observed between VTSP and R6 strains during the phases of growth analyzed. The absence of changes in pep27 expression in VTSP strains in the stationary phase may be related to their ability to tolerate high antibiotic concentrations, and thus, they survive and remain in the host under the antibiotic selective pressure reflected in therapeutic failure.
Effect of a professional soccer match in skin temperature of the lower limbs : a case study
Alex de Andrade Fernandes,Eduardo Mendonça Pimenta,Danilo Gomes Moreira,Manuel Sillero-Quintana,João Carlos Bouzas Marins,Rodrigo Figueiredo Morandi,Tane Kanope,Emerson Silami Garcia 한국운동재활학회 2017 JER Vol.13 No.3
The purpose of this study is to analyze the relationship between lower limbs skin temperature (Tsk) recorded by infrared thermography and creatine kinase (CK) response following an official soccer game. A pro-fessional soccer player, from the first division Brazilian League, defender, 27 years and 183 cm of height was analyzed. Measures of Tsk with a thermal camera and blood CK concentrations were obtained 24 hr be-fore (24H-PRE), 24 (24H-POST) and 48 hr (48H-POST) after an official match. The results showed that CK values were 193 U/L 24 hr before, ris-ing to 1,083 U/L 24 hr after the game and 414 U/L 48 hr after the game. Anterior thigh Tsk was 31.5ºC, 33.8ºC, and 31.8ºC in the moments 24H-PRE, 24H-POST, and 48H-POST, respectively. Similarly, anterior leg presented temperature of 31.2ºC, 33.3ºC, and 31.5ºC at the same moments. Qualita-tive analysis of the thermograms showed that whole lower limbs are much warmer 24 hr after the match and certain areas as the right anteri-or thigh, the left anterior leg, both anterior ankles, and both posterior thighs have not fully recovered their initial Tsk 48 hr after the match. The results of this study indicate that participation in a professional soccer match can lead to significant increases in Tsk values measured by IRT 24 hr after the match. Considering this variable as an indicator of muscu-lar damage, it could help in the process of training control, being part of an injury prevention program in professional soccer clubs.
Production system influences color stability and lipid oxidation in gluteus medius muscle
Salim Ana Paula Amaral de Alcântara,Ferreira Micheli da Silva,Monteiro Maria Lucia Guerra,Lima Loíse Caroline Santos de,Magalhães Isabelle Trezze Marins,Conte-Júnior Carlos Adam,Mano Sérgio Borges 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.5
Objective: We aimed to evaluate the color and oxidative stability of beef gluteus medius (GM) from cattle raised in organic and non-organic production systems. Methods: The GM samples (n = 10) were obtained from organic (ORG; n = 5) or nonorganic (NORG; n = 5) beef samples, sliced into 2.54-cm steaks, packaged in aerobic conditions, and stored for nine days at 4°C. ORG and NORG steaks were compared regarding myoglobin concentration, pH, instrumental color, delta E (ΔE), metmyoglobin reducing activity (MRA), and lipid oxidation on days 0, 5, and 9. Results: Feeding system did not influence (p>0.05) the myoglobin concentration. ORG steaks exhibited greater (p<0.05) meat pH, yellowness, and MRA, whereas NORG steaks exhibited greater (p<0.05) redness, chroma, R630/580, delta E, and lipid oxidation. ORG and NORG steaks exhibited similar (p>0.05) lightness and hue angle. During storage, ORG and NORG exhibited an increase in muscle pH, hue angle, and lipid oxidation; and a decrease (p<0.05) in redness, yellowness, chroma, and color stability (R630/580). Both samples exhibited a stable (p>0.05) pattern for lightness and MRA. Conclusion: Therefore, the production system can affect beef color and lipid stability during storage.