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Carlos Roncero,Raul Felipe Palma-Álvarez,Elena Ros-Cucurull,Carmen Barral,Begoña Gonzalvo,Margarida Corominas-Roso,Miguel Casas,Lara Grau-López 대한정신약물학회 2016 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.14 No.1
Brain-derived neurotrophic factor (BDNF) is linked to numerous brain functions. In addition, BDNF alterations contribute to neurological, mental, and addictive disorders. Cocaine dependence has received much attention recently due to its prevalence and psychological effects. Symptoms of psychosis are one of the most serious adverse events precipitated by cocaine use. It is particularly important to identify patients at risk of developing cocaine-induced psychosis (CIP). We described two cases of patients with cocaine dependence who presented with CIP and had changes in their BDNF levels during the psychotic episode. BDNF levels were initially low in both patients, and then decreased by more than 50% in association with CIP. The relationship between BDNF and psychosis is described in the literature. These cases revealed that BDNF levels decreased during a CIP episode and, thus, it is necessary to investigate BDNF and its relationship with CIP further.
Neural Transdifferentiation: MAPTau Gene Expression in Breast Cancer Cells
Lara-Padilla, E,Miliar-Garcia, A,Gomez-Lopez, M,Romero-Morelos, P,Bazan-Mendez, CI,Alfaro-Rodriguez, A,Anaya-Ruiz, M,Callender, K,Carlos, A,Bandala, C Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4
Background: In tumor cells, aberrant differentiation programs have been described. Several neuronal proteins have been found associated with morphological neuronal-glial changes in breast cancer (BCa). These neuronal proteins have been related to mechanisms that are involved in carcinogenesis; however, this regulation is not well understood. Microtubule-associated protein-tau (MAP-Tau) has been describing in BCa but not its variants. This finding could partly explain the neuronal-glial morphology of BCa cells. Our aim was to determine mRNA expression of MAP-tau variants 2, 4 and 6 in breast cancer cell lines. Materials and Methods: Cultured cell lines MCF-10A, MDA-MB-231, SKBR3 and T47D were observed under phase-contrast microscopy for neural morphology and analyzed for gene expression of MAP-Tau transcript variants 2, 4 and 6 by real-time PCR. Results: Regarding morphology like neural/glial cells, T47D line shown more cells with these features than MDA-MB-231 and SKBR. In another hand, we found much greater mRNA expression of MAP-Tau transcript variants 2, and to a lesser extent 4 and 6, in T47D cells than the other lines. In conclusion, regulation of MAP-Tau could bring about changes in cytoskeleton, cell morphology and motility; these findings cast further light on neuronal transdifferentiation in BCa.
Heat Treatment and Lubrication Analysis Over Surface Integrity of Cold Extruded Metals
Thiago Luiz Lara Oliveira,Frederico Ozanan Neves,Thalita Cristina De Paula,Antonio Carlos Ancelotti Junior 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.2
Cold forming is a process which is largely employed in industry. Thisprocess may have impacts on mechanical properties and surface integrity. This paper presents a study on Surface Integrity (SI) within the context of cold extruded billets with two different materials, steel and aluminium alloys. Different lubricant oils were used in this study, and heat treatment was employed after cold processing. Typical surface alterations such as micro-hardness and roughness were analysed, and a FEM analysis was used to predict the areas with increasing hardness. An analysis of test variance (ANOVA) was conducted, aiming to determine the influence factors of the experiments. Thus, influence factors like oil performance and extrusion load were investigated. The performance and influence of oils on cold extrusion processing for different materials were analysed. Due to the effects of lubricants on surface integrity in terms of hardness and roughness, a comparison was given with projections forsubstituting mineral oils for vegetable oils without losing functional performance.
( Alvaro R. Lara ),( Janet Galindo ),( Karim E. Jaen ),( Mariana Juarez ),( Juan-carlos Sigala ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10
The aerobic growth and metabolic performance of Escherichia coli strains BL21 and W3110 were studied when the Vitreoscilla hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo<sub>3</sub> genes only in BL21. These results are useful for better selecting a production host.
90-Day Oral Toxicity Assessment of Tropaeolum majus L. in Rodents and Lagomorphs
Valdinei de Oliveira Araujo,Carlos Eduardo Linhares Andreotti,Michelle de Paula Reis,Daniely Alves de Lima,Karoline Bach Pauli,Bruna Caroline Nunes,Caroline Gomes,Ricardo de Melo Germano,Euclides Lara 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.8
Tropaeolum majus L., popularly known as nasturtium, is a species widely used in the form of infusions and salads. In the last years, the antihypertensive, diuretic, and calcium and potassium sparing activities of T. majus preparations were shown. Moreover, no preclinical 90-day oral toxicity studies were conducted. Thus, this study evaluated the toxicity of the hydroethanolic extract obtained from T. majus (HETM) leaves in female and male mice, rats, and rabbits. Swiss mice and Wistar rats were treated with HETM (75, 375, and 750 mg/kg). The doses of rabbits (30, 150, and 300 mg/kg) were calculated by allometric extrapolation. The control groups received vehicle. The animals were orally treated, daily, for 90 days. At the end, the animals were anesthetized, and body weight gain, relative weight of liver, kidney, and spleen, and histopathological changes were evaluated. Serum hematological and biochemical parameters were also analyzed. No alterations were found in body and organ weights or in histopathological and biochemical evaluation. Hematological analyses revealed small changes in lymphocytes and neutrophil counts in rats after administration of 750 mg/kg of HETM. These results showed that 90-day use of T. majus is safe in rodents and lagomorphs.
Berenise Borges-Contreras,Cecilia Eugenia Martı´nez-Sa´nchez,Erasmo Herman-Lara,Jesu´s Rodrı´guez-Miranda,Betsabe´ Herna´ndez-Santos,Jose´ Manuel Jua´rez-Barrientos,Carlos Martı´n Guerra-Almonacid,Dav 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.3
Mojarra of Nile tilapia (Oreochromis niloticus) skeleton was used as protein source for the preparation of protein hydrolysates and peptide fractions with angiotensin-converting enzyme (ACE) inhibitory activity. The flour presented a content of 34.92% protein and a brightness (luminosity, L*) of 82.29. Protein hydrolysates were obtained from the protein-rich flour with the enzymes Flavourzyme® and Alcalase® reaching degree of hydrolysis (%DH) of 52% and 67% at 100 min of reaction, respectively. Both hydrolysates showed low-molecular-weight (MW) peptides estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The hydrolysates obtained with Flavourzyme at 60 min and at 80 min with Alcalase showed greater ACE inhibitory activity with IC50 values of 0.238 and 0.344 mg/mL, respectively. The peptide fraction A (MW >10 kDa) with Flavourzyme and fraction B (MW = 10–5 kDa) with Alcalase obtained by ultrafiltration of hydrolysates with higher DH presented IC50 of 0.728 and 0.354 mg/mL, respectively, whereas peptide fraction C (MW = 5–3 kDa) with both enzymes hydrolysates with greater ACE inhibitory activity showed IC50 values of 0.470 and 0.634 mg/mL. The components obtained in this study could be used as functional ingredients in the design and development of functional foods.
Blanca Patricia Lazalde-Ramos,Ana Lourdes Zamora-Perez,Ayme´e Ileana Ortega-Guerrero,Saira Zulema Quintero-Fraire,Omar Palacios-Lara,Sol Marı´a Quirarte-Baez,Carlos Galaviz-Hernandez,Martha Sosa-Macıa 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.10
Genomic instability is associated with increased oxidative stress in patients with human immunodeficiency virus (HIV). The aim of this study was to determine the effect of intake of methanolic and aqueous extracts of Rosmarinus officinalis on genomic instability in HIV patients. We studied 67 HIV patients under pharmacological treatment with ATRIPLA who were divided into three groups: group 1, patients under ATRIPLA antiretroviral therapy; group 2, patients with ATRIPLA and rosemary aqueous extract (4 g/L per day); and group 3, patients with ATRIPLA and rosemary methanolic extract (400 mg/day). The genomic instability was evaluated through the buccal micronucleus cytome assay. Oral epithelial cells were taken at the beginning and 1 and 4 months later. The groups that received the pharmacological therapy with ATRIPLA and the complementary therapy with R. officinalis extracts showed a decrease in the number of cells with micronuclei and nuclear abnormalities compared with the group that only received ATRIPLA. The complementary therapy with R. officinalis decreased the genomic instability in HIV patients.