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RISS 인기검색어
- 검색키워드
- 저자 : Carl-Fredrik, W (검색결과 4 건)
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Michael Fritzsche,Carl-Fredrik Mandenius,Joachim Fritzsche,Jonas O. Tegenfeldt 한국바이오칩학회 2014 BioChip Journal Vol.8 No.2
Fabrication and application of a non-fluorescent and UV-transparent microfluidic biochip in fused silica that allows sensitive autofluorescence detection are described. The biochip is particularly useful in cell-based assays where the most informative autofluorescence signals from the cells reside in the ultraviolet spectral range and where plastic labware materials commonly used in cell culture work severely disturb such measurements. In this study the fused silica biochip was used for measuring intrinsic autofluorescence from liver cells in order to assess hepatotoxic effects of drugs. The assessment assay was carried out with the human liver cell line HepG2 under perfusionconditions in the microfluidics of the biochip. The autofluorescence from the liver cells exposed to quinidine was readily recorded without background disturbance and correlated well with reference toxicity data.
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A Microbore Tubing Based Spiral for Multistep Cell Fractionation
Pasitka, Laura,van Noort, Danny,Lim, Wanyoung,Park, Sungsu,Mandenius, Carl-Fredrik American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.21
<P>Cells were separated with the aid of a multistep spiral fractionation device, utilizing hydrodynamic forces in a spiral tubing. The spiral was fabricated using “off-the-shelf” microbore tubing, allowing for cheap and fast prototyping to achieve optimal cell separation. As a first step, a model system with 20 and 40 μm beads was used to demonstrate the effectiveness of the multistep separation device. With an initial purity of 5%, a separation purity of 83% was achieved after a two-step separation with the addition of 0.1% polyethylene glycol (PEG)-8000. Next, doxorubicin-resistant polyploid giant breast cancer cells (MDA-MB-231) were separated from doxorubicin-sensitive monoploid small breast cancer cells in the same fashion as the beads, resulting in a purity of around 40%, while maintaining a cell viability of more than 90%. Combined with basic cell analytical methods, the hydrodynamic separation principle of the device could be envisaged to be useful for a variety of cell fractionation needs in cell biology and in clinical applications.</P> [FIG OMISSION]</BR>
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Oh, Jungsu S.,Marek, K,Gudrun, R,Sylvain, B,Levitt, James J.,McCarley, Robert W.,Carl-Fredrik, W,Shenton, Martha E. Wiley Subscription Services, Inc., A Wiley Company 2009 Human brain mapping Vol.30 No.11
<P>Diffusion tensor imaging (DTI) and fiber tractography are useful tools for reconstructing white matter tracts (WMT) in the brain. Previous tractography studies have sought to segment reconstructed WMT into anatomical structures using several approaches, but quantification has been limited to extracting mean values of diffusion indices. Delineating WMT in schizophrenia is of particular interest because schizophrenia has been hypothesized to be a disorder of disrupted connectivity, especially between frontal and temporal regions of the brain. In this study, we aim to differentiate diffusion properties of thalamo-frontal pathways in schizophrenia from normal controls. We present a quantitative group comparison method, which combines the strengths of both tractography-based and voxel-based studies. Our algorithm extracts white matter pathways using whole brain tractography. Functionally relevant bundles are selected and parsed from the resulting set of tracts, using an internal capsule (IC) region of interest (ROI) as “source”, and different Brodmann area (BA) ROIs as “targets”. The resulting bundles are then longitudinally parameterized so that diffusion properties can be measured and compared along the WMT. Using this processing pipeline, we were able to find altered diffusion properties in male patients with chronic schizophrenia in terms of fractional anisotropy (FA) decreases and mean diffusivity (MD) increases in precise and functionally relevant locations. These findings suggest that our method can enhance the regional and functional specificity of DTI group studies, thus improving our understanding of brain function. Hum Brain Mapp, 2009. © 2009 Wiley-Liss, Inc.</P>
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Yoo, Seung-Schik,Park, Hae-Jeong,Soul, Janet S.,Mamata, Hatsuho,Park, HyunWook,Westin, Carl-Fredrik,Bassan, Haim,Du Plessis, Adre J.,Robertson Jr, Richard L.,Maier, Stephan E.,Ringer, Steven A.,Volpe, J.B. Lippincott 2005 Vol. No.
OBJECTIVE:: The goal of this study was to test the feasibility of visualizing a 3-dimensional structure of cerebral white matter fiber tracts in preterm infants, postconceptional age (PCA) 28 weeks to term, by using volumetric diffusion tensor magnetic resonance imaging (DTI) data. MATERIALS AND METHOD:: We combined tractography algorithms and visualization methods, currently available for adult DTI data, to trace the pixelated principal direction of a diffusion tensor originating from regions-of-interest with high fractional anisotropy. Consequently, white matter fiber bundles from the genu and the splenium of corpus callosum, the corticospinal tracts, the inferior fronto-occipital fasciculi, and optic radiations were visualized. RESULTS:: Our results suggest that major white matter tracts of preterm infant brains, with PCAs ranging from 28 weeks to term (40 weeks old), can be successfully visualized despite the small brain volume and low anisotropy. CONCLUSION:: The feasibility of fiber tractography in preterm neonates with DTI may add a new dimension in detection and characterization of white matter injuries of preterm infants.
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