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        Colorimetric Detection of Cr3+ in Aqueous Solution Based on Confunctionalized Silver Nanoparticles Modified with 4-Nitrobenzenethiol and 4-Mercaptobenzoic Acid

        Zhikun Zhang,Ying Zhou,Jing-Kui Yang,Peilong Wang,Xiaoou Su,Hong Zhao,Yujian He,Zhiqin Cao,Maoqiang Luo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7

        A new method has been proposed to realize the visual detection of Cr3+ using 4-nitrobenzenethiol (4-NBT) and 4-mercaptobenzoic acid (4-MBA) modified silver nanoparticles (AgNPs). The presence of Cr3+ induces the aggregation of AgNPs through cooperative metal–ligand interaction, resulting in a color change from bright yellow to purple. Consequently, Cr3+ could be monitored by colorimetric response of AgNPs by a UV-Vis spectrophotometer or even naked eyes. We firstly used ethylene diamine tetraacetic acid (EDTA) as a masking agent to selectively detect Cr3+, and other metal ions have little influence on the Cr3+–AgNPs system. The cofunctionalized AgNPs exhibited a highly sensitive detection limit of Cr3+, which is as low as 5 x 10-9 mol L-1, and the absorbance ratio (A600nm /A387nm) is linear with the concentration of Cr3+ ranging from 5 x 10-9 mol L-1 to 2 x 10-6 mol L-1 with a coefficient of 0.993. Particularly, the sensor has been further evaluated to monitor the concentration of Cr3+ in drinking water, the recovery was in good agreement with those obtained by ICP-MS, indicating that this proposed method is successfully applied in real samples.

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        Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

        Shen Wenzheng,Fu Yu,Bai Haiyu,Zhang Zhiyu,Cao Zhikun,Liu Zibo,Yang Chao,Sun Shixin,Wang Lei,Ren Chunhuan,Ling Ying-hui,Zhang Zi Jun,Cao Hongguo 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

        Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

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