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高麗人蔘에 育種의 關한 硏究 : Panax ginseng C. A. Meyer
李載斗,洪性式 成均館大學校 1980 論文集 Vol.27 No.-
This study is the report of karyotype analysis in somatic cell and the investigation of chromosomal aberration was induced by growth substances, X-ray and colchicine as mutagen in tissue culture of Korean ginseng. We obtained the following results. For the karyotype analysis, we used embryo in the seedling and shoot bud, the number of chromsomes(2n) is 48. Of these 46 make pair showning that these are A-chromosome, the homologous chromosome are identified from the centromere and chromosome band and rest of them which correspond to B-chromosome did not form pair corresponds to B-chromosome. It is interresting that one of the B-chromosome is the largest and the other is the smallest. For the induction of artificial mutation, we used growth substances as 2,4-dichlorophenoxy acetic acid(2,4-D) and cytokinin, and X-ray and colchicine. This treatment induce the variety of chromosome number. Sample cultured in Murashige-Skoog media contained growth substances show mostly diploid chromosome and there were some haploid (n=24) and aneuploid. Among the aneuploid observed are 2n-2, 2n+4 or 2n+12. In addition to this, there were tetraploid (4n=96) and their aneuploid (4n-4, 4n-1 and 4n+6). However, X-ray irradiation cause n and 4n disappear and some aneuploid of 2n type (2n-8, 2n-2 and 2n+2) and 2n phase. Next, we observed the effect of colchicine in the suspension culture, in this experiment we recognized n, 2n and their aneuploid. However, we did not observed tetraploid. The variation from n to 2n is gradual and there is no 4n indicating that the furture experiment of cytological mechanism of mutation induced by growt-h substances will be useful of developing new breed of Korean ginseng.
李載斗,洪性式 成均館大學校 1978 論文集 Vol.25 No.-
The object of this study is to clarify the effects of single and combination treatments of α-naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA) and kinetin in young stem cultures of Paeonia albiflora. Basic medium was Murashige & Skoog's medium, and all media were adjusted to pH 5.8 before autoclaving. This experiments were carried out in the dark room and it was kept with temperature of 25±1℃. In single treatment of above various growth substances, the optimum concentrations on callus initiation were NAA of 0.5mg/l,2,4-D of 0.5mg/l,IAA of 0.05mg/l and kinetin of 0.3mg/l. In combination treatments of above various growth substances 2,4-D+kinetin would be very efficient to clone formation, but did notinduce root differentiation. And, it's suggested that the effects of 2.4-D+kinetin+NAA, 2,4-D+kinetin+IAA and 2,4-D+kinetin+NAA+IAA were same as 2,4-D+kinetin, and NAA+2,4-D showed clone formation efficiently. Also, adding of NAA or 2,4-D established clone formation and root differentiation. On the other hand, NAA+kinetin would be effective to root differentiation. Nevertheless, excellent root differentiation and development would be established from the clones in which was trasfered from basic medium+NAA (0.5mg/l) to basic medium.
새로운 의약품의 합성에 관한 연구 : 6-Thiocyanopenicillanic Acid 유도체의 합성
李英行,李寀浩,崔元植 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3
6-Aminopenicillanic acid의 diazo화 반응과 sodium thiocyanate와 반응으로 새로운 β-lactam 화합물인 6-thiocyanopenicillanic acid(1a)를 합성하고 p-nitrobenzyl bromide, chloromelthylpivalate 및 1-chlorodiethylcarbonate들과의 반응에 의해 각각에 해당하는 ester(1b~d)를 합성하였다. A new β-lactam compound, 6-thiocyanopenicillanic acid(1a) was prepared by the reaction of diawnium salt of 6-aminopenicillanic acid with sodium thiocyanate. Reaction of 6-thiocyanopenicillanic acid(la) with p-nitrobenzyl bromide, chloromethylpivalate and l-chlorodiethylcarbonate, respectively, afforded the corresponding esters(1b~d).
The Rise of Neo-Confucian Orthodoxy and the Problem of Conformism in Yi Korea
CHUNG, CHAI-SIK Korean Social Science Research Council Korean Nati 1985 Korean Social Science Journal Vol.12 No.1
If there was anything really distinctive about the Cuh Hsi Neo-Confucianism in Yi society, it perhaps would be the "Four Beginnings and Seven Feelings"(sadan ch'ilchong, in Chinese ssu-tuan-ch'ich'ing) debate on the nature and function of the human mind that preoccupied most of the intellectuals for several hundred years. It can be said that the mainstream of the Yi intellectual tradition was a series of footnotes to what Yi Hwang(T'oegye, 1501-1570) and Yi I(Yulgok, 1536-1584), the two foremost scholars of Neo-Confucianism in Korea, had said about this issue. It has been suggested that most of the scholars' intellectual resources were consumed in debating the issue. The paper briefly examines the debate and some of the salient features of Korean Neo-Confucian orthodoxy as they developed in the context of the debate