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RISS 인기검색어
- 검색키워드
- 저자 : Bunick, David (검색결과 2 건)
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Lee, Ki-Ho,Bunick, David,Lamprecht, Georg,Choi, Inho,Bahr, Janice M. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.4
Our earlier studies showed that estrogen was involved in the regulation of fluid reabsorption in adult mouse efferent ductules (ED), through estrogen receptor (ER) ${\alpha}$ and $ER{\beta}$ by modulating gene expression of epithelial genes involved in ion homeostasis. However, little is known about the importance of $ER{\alpha}$ in the ED during postnatal development. Based on previous findings, we hypothesized that there should be a difference in the expression of epithelial ion transporters and anion producers in the ED of postnatal wild type (WT) and estrogen receptor ${\alpha}$ knockout (${\alpha}ERKO$) mice. Using absolute, comparative and semi-quantitative RT-PCR along with immunohistochemistry, we looked at expression levels of several genes in the ED across postnatal development. The presence of estrogen in the testicular fluid was indirectly ascertained by immunohistochemical detection of the P450 aromatase in the testis. There was no immunohistochemically detectable difference in the expression of P450 aromatase in the testes and ER${\beta}$ in the ED of WT and ${\alpha}$ERKO mice. ER${\alpha}$ was only detected in the ED of WT mice. The absence of ER${\alpha}$ in the ED of postnatally developing mice resulted in differential expression of mRNAs and/or proteins for carbonic anhydrase II, $Na^+/H^+$ exchanger 3, down-regulated in adenoma, cystic fibrosis transmembrane regulator, and $Na^+/K^+$ ATPase ${\alpha}$. Our data indicate that the absence of ER${\alpha}$ resulted in altered expression of an epithelial ion producer and transporters during postnatal development of mice. We conclude that the presence of ER${\alpha}$is important for regulation of the ED function during the prepubertal developmental and postpubertal period.
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Lee, Ki-Ho,Park, Jae-Hwa,Bunick, David,Lubahn, Dennis B.,Bahr, Janice M. Blackwell Publishing Ltd 2009 Journal of anatomy Vol.214 No.6
<P>Abstract</P><P>Estrogen and the estrogen receptor (ER)α play an important role in the male reproductive tract and in fertility. Previous studies demonstrated that disruption of ERα function resulted in abnormal morphology of the testis and efferent ductules (EDs) of adult mice. However, the effect of a lack of a functional ERα during early postnatal development has not been determined. The present study is an evaluation of morphological changes effected by a lack of ERα in the male reproductive tract during the postnatal period. Age-equivalent wild-type and ERα knockout (αERKO) mice at 10, 18, 35 and 60 days of age after birth were used for morphological comparison of the testes and ED. Light microscopic examination of the testes of the αERKO mouse revealed a dramatic dilation of the rete testis as early as 10 days of age, premature lumen formation, reduced epithelial height and greatly dilated lumen of seminiferous tubules as early as 18 days of age. The proximal ED of the αERKO mouse showed lumen dilation, reduction of epithelial height and a decrease of nuclear height as early as 10 days of age. Similar, but somewhat less severe, morphological abnormalities were observed in the distal ED of the αERKO mouse. These results indicate that a lack of functional ERα leads to morphological changes of the testis and ED of the early postnatal developing mouse. Based on these observations, we conclude that ERα plays an important role in normal development of the testis and ED, not only during adulthood but also during the entire postnatal period and presumably during fetal development.</P>
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