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Effect of Temperature on Oxidation Kinetics of Walnut and Grape Seed Oil
Bipin Vaidya,은종방 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
The effects of temperature on the oxidation of walnut oil (WO) and grape seed oil (GSO) were investigated. Both oils were stored at 25, 40, 60, and 80oC for 60, 30, 12,and 6 day, respectively. Degree of oxidation was measured in terms of peroxide value (POV), conjugated dienoic acid (CDA) value, and p-anisidine value (p-AV). The rates of increase in POV, CDA, and p-AV in both oils were strongly dependent on storage temperature. The induction period (IP) decreased gradually with increasing storage temperature in both oils. WO showed shorter IPs (23.0,9.2, and 1.0 day at 25, 40, and 60oC, respectively) than those of GSO (41.5, 12.0, and 1.8 day at 25, 40, and 60oC,respectively), indicating the higher susceptibility of WO to oxidation. The activation energy (Ea) for the IP of WO and GSO autoxidation was similar (75.87 and 75.66 kJ/mol,respectively), revealing similar temperature dependence for autoxidation in WO and GSO.
Bipin Vaidya,최은옥 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.1
This study compared stabilities of tocopherols and lutein in oil extracted from roasted mustard seeds (RMSO) with those in oil from unroasted seeds (URMSO)during oil oxidation at 60^oC in the dark for 12 days. Tocopherols and lutein were determined by high performance liquid chromatography, and the oil oxidation was monitored with conjugated dienoic acid (CDA) content and fatty acid composition by gas chromatography. The rate of CDA increase was lower in RMSO (0.038%/day) than in URMSO (0.047%/day) during 12-day oxidation in the dark, with little change in fatty acid composition in both oils. Tocopherols and lutein were more abundant in RMSO,initially 465.38 and 100.55 μg/g, respectively, than in URMSO, and their stability was higher in RMSO (−4.63and −5.94%/day of degradation in tocopherols and lutein,respectively) than in URMSO during 12-day oxidation of oil, both of which contributed to higher autoxidative stability of RMSO than URMSO.
Human Norovirus Replication in Temperature-Optimized MDCK Cells by Forkhead Box O1 Inhibition
정은혜,조세영,Bipin Vaidya,하상훈,전상미,노현주,이유정,이주혜,권요셉,김두운 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.9
Human noroviruses (HuNoVs) are a leading cause of gastroenteritis outbreaks worldwide. However, the paucity of appropriate cell culture models for HuNoV replication has prevented developing effective anti-HuNoV therapies. In this study, first, the replication of the virus at various temperatures in different cells was compared, which showed that lowering the culture temperature from 37°C significantly increased virus replication in Madin-Darby canine kidney (MDCK) cells. Second, the expression levels of autophagy-, immune-, and apoptosis-related genes at 30°C and 37°C were compared to explore factors affecting HuNoV replication. HuNoV cultured at 37°C showed significantly increased autophagy-related genes (ATG5 and ATG7) and immune-related genes (IFNA, IFNB, ISG15, and NFKB) compared to mock. However, the virus cultured at 30°C showed significantly decreased expression of autophagy-related genes (ATG5 and ATG7), but not significantly different major immune-related genes (IFNA, ISG15, and NFKB) compared to mock. Importantly, expression of the transcription factor FOXO1, which controls autophagy- and immune-related gene expression, was significantly lower at 30°C. Moreover, FOXO1 inhibition in temperature-optimized MDCK cells enhanced HuNoV replication, highlighting FOXO1 inhibition as an approach for successful virus replication. In the temperature-optimized cells, various HuNoV genotypes were successfully replicated, with GI.8 showing the highest replication levels followed by GII.1, GII.3, and GII.4. Furthermore, ultrastructural analysis of the infected cells revealed functional HuNoV replication at low temperature, with increased cellular apoptosis and decreased autophagic vacuoles. In conclusion, temperature-optimized MDCK cells can be used as a convenient culture model for HuNoV replication by inhibiting FOXO1 and providing adaptability to different genotypes.
KO, SANG-MU,VAIDYA, BIPIN,KWON, JOSEPH,LEE, HEE-MIN,OH, MYUNG-JOO,SHIN, TAI-SUN,CHO, SE-YOUNG,KIM, DUWOON International Association for Food Protection 2015 Journal of food protection Vol.78 No.5
<P>Outbreaks of hepatitis A virus (HAV) infections are most frequently associated with the consumption of contaminated oysters. A rapid and selective concentration method is necessary for the recovery of HAV from contaminated oysters prior to detection using PCR. In this study, ricin extracted from castor beans (Ricinus communis) was tested as an alternative to antibody used in immunomagnetic separation while concentrating HAV prior to its detection using reverse transcription PCR. Initially, the extracted proteins from castor beans were fractionated into 13 fractions by gel filtration chromatography. Pretreatment of different protein fractions showed a variation in binding of HAV viral protein (VP) 1 to oyster digestive tissue in the range of 25.9 to 63.9%. The protein fraction, which caused the highest reduction in binding of VP1 to the tissue, was identified as ricin A by quadrupole time-of-flight mass spectrometry. Ricin A could significantly inhibit binding of VP1 to the tissue with a 50% inhibitory concentration of 4.5 μg/ml and a maximal inhibitory concentration of 105.2%. The result showed that the rate of inhibition of HAV binding to tissue was higher compared to the rate of ricin itself binding to HAV (slope: 0.0029 versus 0.00059). However, ricin A concentration showed a higher correlation to the relative binding of ricin itself to HAV than the inhibition of binding of HAV to the tissue (coefficient of determination, R2: 0.9739 versus 0.6804). In conclusion, ricin A-linked magnetic bead separation combined with reverse transcription PCR can successfully detect HAV in artificially seeded oyster digestive tissue up to a 10−4 dilution of the virus stock (titer: 104 50% tissue culture infective dose per ml).</P>
김송학,Susanne U. Mertens-Talcott,Bipin Vaidya,Vinicius Paula Venancio,조세영,송종암,Boon P. Chew,권조셉,김두운 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.12
Quantitative reverse transcription PCR (qRTPCR)is a sensitive method for the detection of foodborneviruses in fecal samples. However, the performance ofqRT-PCR depends on the efficiency of virus concentrationmethods. In this study, the effect of Concanavalin A (ConA)-immobilized on polyacrylate beads (Con A-PAB) onthe qRT-PCR performance, in terms of sensitivity andspecificity to detect foodborne viruses in human fecalspecimens was compared with commercial viral RNAextraction kit (VRNA). The detection of foodborne virusesby qRT-PCR was validated by viral genome sequencing. Both Con A-PAB and VRNA methods were equally sensitiveand specific for detecting hepatitis A virus in fecalspecimens. Even though both methods showed highspecificity (100% vs. 100%) for detecting human norovirus(HuNoV), Con A-PAB method exhibited higher sensitivity(100% vs. 42.9%) and accuracy (100% vs. 73.3%) comparedto VRNA method. In conclusion, the application ofCon A-PAB would improve the performance of qRT-PCRfor the detection of HuNoV in fecal samples.