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Simplified robustness assessment of steel framed structures under fire-induced column failure
Binhui Jiang,Guo-Qiang Li,Michael C.H. Yam 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.35 No.2
This paper proposes a Global-Local Analysis Method (GLAM) to assess the progressive collapse of steel framed structures under fire-induced column failure. GLAM obtains the overall structural response by combining dynamic analysis of the heated column (local) with static analysis of the overall structure (global). Test results of two steel frames which explicitly consider the dynamic effect during fire-induced column failure were employed to validate the proposed GLAM. Results show that GLAM gives reasonable predictions to the test frames in terms of both whether to collapse and the displacement verse temperature curves. Besides, several case studies of a two-dimensional (2D) steel frame and a three-dimensional (3D) steel frame with concrete slabs were conducted by using GLAM. Results show that GLAM gives the same collapse predictions to the studied cases with nonlinear dynamic analysis of the whole structure model. Compared with nonlinear dynamic analysis of the whole structure model, GLAM saves approximately 70% and 99% CPU time for the cases of 2D and 3D steel frame, respectively. Results also show that the load level of a structure has notable effects on the restraint condition of a heated column in the structure.
Wang, Yechun,Guo, Binhui,Zhang, Fei,Yao, Hongyan,Miao, Zhiqi,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6
The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of CgHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that CgHMGR belonged to a small gene family. Expression analysis revealed that CgHMGR expressed high in roots, and low in leaves and stems, and the expression of CgHMGR could be up-regulated by methyl jasmonate (MeJA). The functional color assay in Escherichia coli showed that CgHMGR could accelerate the biosynthesis of $\beta$-carotene, indicating that CgHMGR encoded a functional protein. The cloning, characterization and functional analysis of CgHMGR gene will enable us to further understand the role of CgHMGR involved in taxol biosynthetic pathway in C. avellana at molecular level.
Kai, Guoyin,Zhao, Lingxia,Zhang, Lei,Li, Zhugang,Guo, Binhui,Zhao, Dongli,Sun, Xiaofen,Miao, Zhiqi,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6
A full-length cDNA encoding taxadiene synthase (designated as TmTXS), which catalyzes the first committed step in the Taxol biosynthetic pathway, was isolated from young leaves of Taxus media by rapid amplification of cDNA ends (RACE). The full-length cDNA of TmTXS had a 2586 bp open reading frame (ORF) encoding a protein of 862 amino acid residues. The deduced protein had isoelectric point (pI) of 5.32 and a calculated molecular weight of about 98 kDa, similar to previously cloned diterpene cyclases from other Taxus species such as T. brevifolia and T. chinenisis. Sequence comparison analysis showed that TmTXS had high similarity with other members of terpene synthase family of plant origin. Tissue expression pattern analysis revealed that TmTXS expressed strongly in leaves, weak in stems and no expression could be detected in fruits. This is the first report on the mRNA expression profile of genes encoding key enzymes involved in Taxol biosynthetic pathway in different tissues of Taxus plants. Phylogenetic tree analysis showed that TmTXS had closest relationship with taxadiene synthase from T. baccata followed by those from T. chinenisis and T. brevifolia. Expression profiles revealed by RT-PCR under different chemical elicitor treatments such as methyl jasmonate (MJ), silver nitrate (SN) and ammonium ceric sulphate (ACS) were also compared for the first time, and the results revealed that expression of TmTXS was all induced by the tested three treatments and the induction effect by MJ was the strongest, implying that TmTXS was high elicitor responsive.