Down-regulation of SENP1 Expression Increases Apoptosis of Burkitt Lymphoma Cells

Huang, Bin-Bin,Gao, Qing-Mei,Liang, Wei,Xiu, Bing,Zhang, Wen-Jun,Liang, Ai-Bin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To investigate the effect of down-regulation of Sentrin/SUMO-specific protease 1 (SENP1) expression on the apoptosis of human Burkitt lymphoma cells (Daudi cells) and potential mechanisms. Methods: Short hairpin RNA (shRNA) targeting SENP1 was designed and synthesized and then cloned into a lentiviral vector. A lentiviral packaging plasmid was used to transfect Daudi cells (sh-SENP1-Daudi group). Daudi cells without transfection (Daudi group) and Daudi cells transfected with blank plasmid (sh-NC-Daudi group) served as control groups. Flow cytometry was performed to screen GFP positive cells and semiquantitative PCR and Western blot assays were employed to detect the inference efficiency. The morphology of cells was observed under a microscope before and after transfection. Fluorescence quantitative PCR and Western blot assays were conducted to measure the mRNA and protein expression of apoptosis related molecules (caspase-3, 8 and 9). After treatment with $COCl_2$ for 24 h, the mRNA and protein expression of hypoxia inducible factor -$1{\alpha}$ (HIF-$1{\alpha}$) was determined. Results: Sequencing showed the expression vectors of shRNA targeting SENP1 to be successfully constructed. Following screening of GFP positive cells by FCM, semiqualitative PCR showed the interference efficiency was $79.2{\pm}0.026%$. At 48 h after transfection, the Daudi cells became shrunken, had irregular edges and presented apoptotic bodies. Western blot assay revealed increase in expression of caspase-3, 8 and 9 with prolongation of transfection (P<0.05). Following hypoxia treatment, mRNA expression of HIF-$1{\alpha}$ remained unchanged in three groups (P>0.05) but the protein expression of HIF-$1{\alpha}$ markedly increased (P<0.05). However, in the sh-SENP1-Daudi group, the protein expression of HIF-$1{\alpha}$ remained unchanged Conclusion: SENP1-shRNA can efficiently inhibit SENP1 expression in Daudi cells. SENP1 inhibition may promote cell apoptosis. These findings suggest that SENP1 may serve as an important target in the gene therapy of Burkitts lymphoma.

A Novel Hybrid Optimization Algorithm Based on GA and ACO for Solving Complex Problem

Bin Gao,Jing-Hua Zhu,Wen-chang Lang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.8

In allusion to the deficiencies of the ant colony optimization algorithm for solving the complex problem, the genetic algorithm is introduced into the ant colony optimization algorithm in order to propose a novel hybrid optimization (NHGACO) algorithm in this paper. In the NHGACO algorithm, the genetic algorithm is used to update the global optimal solution and the ant colony optimization algorithm is used to dynamically balance the global search ability and local search ability in order to improve the convergence speed. Finally, some complex benchmark functions are selected to prove the validity of the proposed NHGACO algorithm. The experiment results show that the proposed NHGACO algorithm can obtain the global optimal solution and avoid the phenomena of the stagnation, and take on the fast convergence and the better robustness.

A Novel Hybrid Evolution Optimization Algorithm and its Application

Bin Gao,Jing-Hua Zhu,Wen-Chang Lang 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.5

For the premature convergence and initial pheromone distribution problem of ant colony optimization algorithm, an improved particle swarm optimization (MPSO) algorithm is introduced into ant colony optimization algorithm in order to propose a novel hybrid evolution optimization (HEACO) algorithm in this paper. In the proposed HEACO algorithm, the ergodicity of the chaos is used to initialize the swarm in order to enhance the diversity of the particle swarm, and adjust the mutation probability and inertia weighting factor in order to improve the capability of local and global search. Then the MPSO algorithm is used to control the parameters of the heuristic factor, pheromone evaporation coefficient, and the stochastic selection threshold in order to effectively overcome the parameter influences of ACO, reduce the numbers of useless experiments and balance the developing optimal solution and enlarging search space. A series of typical traveling salesman problems are selected to validity the effectiveness of the proposed HEACO algorithm. The simulation results show that the performance of the proposed HEACO algorithm is better than the traditional ACO algorithm and PSO algorithm. So the proposed HEACO algorithm can effectively improve the solving efficiency and quality, and speed up the convergence and computation.

Accelerated Split Bregman Method for Image Compressive Sensing Recovery under Sparse Representation

( Bin Gao ),( Peng Lan ),( Xiaoming Chen ),( Li Zhang ),( Fenggang Sun ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.6

Compared with traditional patch-based sparse representation, recent studies have concluded that group-based sparse representation (GSR) can simultaneously enforce the intrinsic local sparsity and nonlocal self-similarity of images within a unified framework. This article investigates an accelerated split Bregman method (SBM) that is based on GSR which exploits image compressive sensing (CS). The computational efficiency of accelerated SBM for the measurement matrix of a partial Fourier matrix can be further improved by the introduction of a fast Fourier transform (FFT) to derive the enhanced algorithm. In addition, we provide convergence analysis for the proposed method. Experimental results demonstrate that accelerated SBM is potentially faster than some existing image CS reconstruction methods.

Lack of Association of Common Polymorphisms in MUC1 Gene with H. pylori Infection and Non-cardia Gastric Cancer Risk in a Chinese Population

Zhang, Bin,Hao, Guang-Yu,Gao, Fang,Zhang, Jian-Zu,Zhou, Cheng-Jiang,Zhou, Li-She,Wang, Ying,Jia, Yan-Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Several lines of evidence support the notion that MUC1 is often aberrantly expressed in gastric cancer, and it is a ligand for Helicobacter pylori. Genetic variation in MUC1 gene may confer susceptibility to H. pylori infection and gastric cancer. We assessed the association of common polymorphisms in MUC1 gene with H. pylori infection and non-cardia gastric cancer using an LD-based tag SNP approach in north-western Chinese Han population. A total of four SNPs were successfully genotyped among 288 patients with non-cardia gastric cancer and 281 age- and sex-matched controls. None of the tested SNPs was associated with H. pylori infection. SNP rs9426886 was associated with a decreased risk of non-cardia gastric cancer, but lost significance after adjustment for multiple testing. Overall, our data indicated that common genetic variations in MUC1 gene might not make a major contribution to the risk of H. pylori infection and non-cardia gastric cancer in our studied population.

Apoptosis of Colorectal Cancer UTC116 Cells Induced by Cantharidinate

Liu, Bin,Gao, Hai-Cheng,Xu, Jing-Wei,Cao, Hong,Fang, Xue-Dong,Gao, Hai-Mei,Qiao, Shi-Xing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Effects of Cantharidinate on apoptosis of human colorectal cancer UTC-116 cells were investigated by means of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, H and E staining, flow cytometry, and Raman Spectra analysis. The results showed Cantharidinate to exert inhibitory action on proliferation of human colorectal cancer UTC-116 cells, inducing apoptosis, arresting cells in G1 phase, with decline of S and G2 phases. In addition, the results of Raman spectrum showed significant changes in the UTC-116 cells chemical structure with stretching after the application of Cantharidinate. Taken together, these results suggest that the treatment of human colorectal cancer with Cantharidinate may be associated with multiple molecular mechanisms for apoptosis. Furthermore, similar to fluorouracil, Cantharidinate should be considered as novel assistant drug for controlling the growth of human colorectal cancer UTC-116 cells.

Viewpoint Unconstrained Face Recognition Based on Affine Local Descriptors and Probabilistic Similarity

( Yong Bin Gao ),( Hyo Jong Lee ) 한국정보처리학회 2015 Journal of information processing systems Vol.11 No.4

Face recognition under controlled settings, such as limited viewpoint and illumination change, can achieve good performance nowadays. However, real world application for face recognition is still challenging. In this paper, we propose using the combination of Affine Scale Invariant Feature Transform (SIFT) and Probabilistic Similarity for face recognition under a large viewpoint change. Affine SIFT is an extension of SIFT algorithm to detect affine invariant local descriptors. Affine SIFT generates a series of different viewpoints using affine transformation. In this way, it allows for a viewpoint difference between the gallery face and probe face. However, the human face is not planar as it contains significant 3D depth. Affine SIFT does not work well for significant change in pose. To complement this, we combined it with probabilistic similarity, which gets the log likelihood between the probe and gallery face based on sum of squared difference (SSD) distribution in an offline learning process. Our experiment results show that our framework achieves impressive better recognition accuracy than other algorithms compared on the FERET database.

인공지능 : 얼굴인식을 위한 어파인 불변 지역 서술자

고용빈 ( Yong Bin Gao ),이효종 ( Hyo Jong Lee ) 한국정보처리학회 2014 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.3 No.9

오늘날 촬영 상황을 조절할 수 있는 환경, 즉 고정된 촬영각이나 일관된 조도 조건에서는 얼굴인식 기술 수준은 신뢰할 수 있을 정도로 높다. 그러나 복잡한 현실에서의 얼굴 인식은 여전히 어려운 과제이다. SIFT 알고리즘은 촬영각의 변화가 미미할 때에 한하여, 크기와 회전 변화에 무관하게 우수한 성능을 보여주고 있다. 본 논문에서는 다양하게 촬영각이 변하는 환경에서도 얼굴 인식을 할 수 있는 어파인불변 지역 서술자를 탐지하는 ASIFT(Affine SIFT)라는 알고리즘을 적용하였다. SIFT 알고리즘을 확장하여 만든 ASIFT 알고리즘은 촬영각 변화에 취약한 단점을 극복하였다. 제안하는 방법에서 ASIFT 알고리즘은 표본 이미지에, SIFT 알고리즘은 검증 이미지에 적용하였다. ASIFT 방법은 어파인 변환을 사용하여 다양한 시각에 따른 영상을 생성할 수 있기 때문에 ASIFT 알고리즘은 저장 영상과 실험 영상의 시각 차이에 따른 문제를 해결할 수 있었다. 실험결과 FERET 데이터를 사용했을 때 제안한 방법은 촬영각의 변화가 큰 경우에 기존의 시프트 알고리즘보다도 높은 인식률을 보여주었다. Under controlled environment, such as fixed viewpoints or consistent illumination, the performance of face recognition is usually high enough to be acceptable nowadays. Face recognition is, however, a still challenging task in real world. SIFT(Scale Invariant Feature Transformation) algorithm is scale and rotation invariant, which is powerful only in the case of small viewpoint changes. However, it often fails when viewpoint of faces changes in wide range. In this paper, we use Affine SIFT (Scale Invariant Feature Transformation; ASIFT) to detect affine invariant local descriptors for face recognition under wide viewpoint changes. The ASIFT is an extension of SIFT algorithm to solve this weakness. In our scheme, ASIFT is applied only to gallery face, while SIFT algorithm is applied to probe face. ASIFT generates a series of different viewpoints using affine transformation. Therefore, the ASIFT allows viewpoint differences between gallery face and probe face. Experiment results showed our framework achieved higher recognition accuracy than the original SIFT algorithm on FERET database.

Evaluation of Airport Capacity through Agent Based Simulation

Yang Peng,Gao Wei,Sun Junqing,Sun Bin 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.6

The operational capacity of an airport must be dimensioned under a long-term strategic view as its productivity is determined by available infrastructure. To predict the airport capacity and satisfy the domestic airport property, the operation procedure of airport is analyzed from the point of Multi-agent system. The proposed agent based architecture is a microscopic model of aircrafts' movement; each aircraft is represented as an agent capable of getting its routing results from instructing agent, and makes its own deconflicition decisions on the basis of a part of the observable scene local to that aircraft. An example of airport capacity evaluation is given, and the result is verified through comparison with SIMMOD. Finally, further research is applied to analysis the reason for delay.

Downregulation of Cdk1 and CyclinB1 Expression Contributes to Oridonin-induced Cell Cycle Arrest at G₂/M Phase and Growth Inhibition in SGC-7901 Gastric Cancer Cells

Gao, Shi-Yong,Li, Jun,Qu, Xiao-Ying,Zhu, Nan,Ji, Yu-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Oridonin isolated from Rabdosia rubescens, a plant used to treat cancer in Chinese folk medicine, is one of the most important antitumor active ingredients. Previous studies have shown that oridonin has antitumor activities in vivo and in vitro, but little is known about cell cycle effects of oridonin in gastric cancer. Materials and Methods: MTT assay was adopted to detect the proliferation inhibition of SGC-7901 cells, the cell cycle was assessed by flow cytometry and protein expression by Western blotting. Results: Oridonin could inhibit SGC-7901 cell proliferation, the $IC_{50}$ being $15.6{\mu}M$, and blocked SGC-7901 cell cycling in the $G_2/M$ phase. The agent also decreased the protein expression of cyclinB1 and CDK1. Conclusions: Oridonin may inhibit SGC-7901 growth and block the cells in the $G_2/M$ phase by decreasing Cdk1 and cyclinB1 proteins.