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Research Articles : Trichoderma asperellum Chi42 Genes Encode Chitinase
( Nguyen Hoang Loc ),( Hoang Tan Quang ),( Nguyen Bao Hung ),( Nguyen Duc Huy ),( Truong Thi Bich Phuong ),( Tran Thi Thu Ha ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Nguyen, Bich Phuong,Kim, Gee Yeong,Jo, William,Kim, Byeong Jo,Jung, Hyun Suk IOP 2017 Nanotechnology Vol.28 No.31
<P>The electrical properties of CH<SUB>3</SUB>NH<SUB>3</SUB>Pb(I<SUB>1−<I>x</I> </SUB>Br<I> <SUB>x</SUB> </I>)<SUB>3</SUB> (<I>x</I> = 0.13) perovskite materials were investigated under ambient conditions. The local work function and the local current were measured using Kelvin probe force microscopy and conductive atomic force microscopy, respectively. The degradation of the perovskite layers depends on their grain size. As the material degrades, an additional peak in the surface potential appears simultaneously with a sudden increase and subsequent relaxation of the local current. The potential bending at the grain boundaries and the intragrains is the most likely reason for the change of the local current surface of the perovskite layers. The improved understanding of the degradation mechanism garnered from this study helps pave the way toward an improved photo-conversion efficiency in perovskite solar cells.</P>
Nguyen, Bich Phuong,Jung, Hye Ri,Ryu, Ka Yeon,Kim, Kyungkon,Jo, William American Chemical Society 2019 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.123 No.51
<P>Charge extraction at carrier transport layers adjacent to perovskites is crucial for the optimization of perovskite solar cells. In particular, Sn-perovskites with no lead elements are known to struggle from charge extraction. Here, we report the effects of organic ligands such as FA and MA (FA = HC(NH<SUB>2</SUB>)<SUB>2</SUB><SUP>+</SUP>; MA = CH<SUB>3</SUB>NH<SUB>3</SUB><SUP>+</SUP>) on charge separation at the interface between electron transport layers and perovskites. TiO<SUB>2</SUB> mesoporous covering the tin-perovskites show significant changes in the electronic structure and built-in potentials according to the ratio of FA to MA. Through a local probe with potential and current mapping, charge transport has been intensively examined. The best cell in this study is obtained as 5.37% at FA/MA = 3:1 with only iodine at the halide sites. Even though the value itself is not comparable with lead halides, it could pave a new direction to improve lead-free perovskite solar cells.</P> [FIG OMISSION]</BR>
Nguyen Ngoc Minh,Duong Men Thi Hoai,Nguyen Phuong Linh,Bui Bich Phuong,Ahn Hee-Chul,Cho Jungsook 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.5
Efonidipine, a calcium channel blocker, is widely used for the treatment of hypertension and cardiovascular diseases. In our preliminary study using structure-based virtual screening, efonidipine was identified as a potential inhibitor of c-Jun N-terminal kinase 3 (JNK3). Although its antihypertensive effect is widely known, the role of efonidipine in the central nervous system has remained elusive. The present study investigated the effects of efonidipine on the inflammation and cell migration induced by lipopolysaccharide (LPS) using murine BV2 and human HMC3 microglial cell lines and elucidated signaling molecules mediating its effects. We found that the phosphorylations of JNK and its downstream molecule c-Jun in LPS-treated BV2 cells were declined by efonidipine, confirming the finding from virtual screening. In addition, efonidipine inhibited the LPS-induced production of pro-inflammatory factors, including interleukin-1β (IL-1β) and nitric oxide. Similarly, the IL-1β production in LPS-treated HMC3 cells was also inhibited by efonidipine. Efonidipine markedly impeded cell migration stimulated by LPS in both cells. Furthermore, it inhibited the phosphorylation of inhibitor kappa B, thereby suppressing nuclear translocation of nuclear factor-κB (NF-κB) in LPS-treated BV2 cells. Taken together, efonidipine exerts anti-inflammatory and anti-migratory effects in LPS-treated microglial cells through inhibition of the JNK/NF-κB pathway. These findings imply that efonidipine may be a potential candidate for drug repositioning, with beneficial impacts on brain disorders associated with neuroinflammation.
Nguyen Bich Thu,Trinh Nam Trung,Do Thi Ha,Nguyen Minh Khoi,Nguyen Viet Than,Thipthaviphone Soulinho,Nguyen Hai Nam,Tran Thi Phuong,배기환 한국생약학회 2010 Natural Product Sciences Vol.16 No.4
The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-a (TNF-a), the mRNA expression levels of pro-inflammatory cytokines, including TNF-a and IL-1b, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-kB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
Bui, Bich Phuong,Nguyen, Phuong Linh,Do, Ha Thi Thu,Cho, Jungsook The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6
Background: Anxiolytic properties of Korean Red Ginseng (KRG) have been previously reported. However, the exact mechanism(s) of action remains to be elucidated. The present study investigated the effect of KRG on immobilization-induced anxiety-like behaviors in mice and explored the involvement of the serotonin and GABA systems and BDNF in the anxiolytic action. Methods: Mice were orally administered with KRG (200 mg/kg/day) for 4 weeks and immobilized once daily for 2 h. p-Chlorophenylalanine (p-CPA) was intraperitoneally injected on day 22-28, and flumazenil or bicuculline was injected on day 25-28. After behavioral evaluations, brains were dissected for biochemical analyses. Results: KRG improved immobilization-induced anxiety-like behaviors in mice, as assessed by the elevated plus maze (EPM) and marble burying tests (MBT). The anxiolytic effect of KRG was comparable to that of fluoxetine, a reference drug clinically used for anxiety disorders. A serotonin synthesis inhibitor, p-CPA, blocked the effect of KRG in the EPM and MBT, indicating the requirement of serotonin synthesis for anxiolytic action. In addition, the anxiolytic effect of KRG was inhibited by bicuculline (a GABA<sub>A</sub> antagonist) in MBT, implying the involvement of GABA transmission. Western blotting analyses revealed that KRG upregulated the expression of tryptophan hydroxylase and GABA<sub>A</sub> receptor in the brain, which was blocked by p-CPA. Enhanced BDNF expression by KRG in the hippocampus was also indicated to mediate the anxiolytic action of KRG in immobilized mice. Conclusion: KRG exhibited the anxiolytic effect in immobilized mice by multiple mechanisms of action, involving enhanced serotonin and GABA transmissions and BDNF expression.
Trichoderma asperellum Chi42 Genes Encode Chitinase
Loc, Nguyen Hoang,Quang, Hoang Tan,Hung, Nguyen Bao,Huy, Nguyen Duc,Phuong, Truong Thi Bich,Ha, Tran Thi Thu The Korean Society of Mycology 2011 Mycobiology Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
The complete mitochondrial genome sequence of the indigenous I pig (Sus scrofa) in Vietnam
Hieu Duc Nguyen,Tuan Anh Bui,Phuong Thanh Nguyen,Oanh Thi Phuong Kim,Thuy Thi Bich Vo 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.7
Objective: The I pig is a long nurtured longstanding breed in Vietnam, and contains excellent indigenous genetic resources. However, after 1970s, I pig breeds have become a small population because of decreasing farming areas and increasing pressure from foreign breeds with a high growth rate. Thus, there is now the risk of the disappearance of the I pigs breed. The aim of this study was to focus on classifying and identifying the I pig genetic origin and supplying molecular makers for conservation activities. Methods: This study sequenced the complete mitochondrial genome and used the sequencing result to analyze the phylogenetic relationship of I pig with Asian and European domestic pigs and wild boars. The full sequence was annotated and predicted the secondary tRNA. Results: The total length of I pig mitochondrial genome (accession number KX094894) was 16,731 base pairs, comprised two rRNA (12S and 16S), 22 tRNA and 13 mRNA genes. The annotation structures were not different from other pig breeds. Some component indexes as AT content, GC, and AT skew were counted, in which AT content (60.09%) was smaller than other pigs. We built the phylogenetic trees from full sequence and D loop sequence using Bayesian method. The result showed that I pig, Banna mini, wild boar (WB) Vietnam and WB Hainan or WB Korea, WB Japan were a cluster. They were a group within the Asian clade distinct from Chinese pigs and other Asian breeds in both phylogenetic trees (0.0004 and 0.0057, respectively). Conclusion: These results were similar to previous phylogenic study in Vietnamese pig and showed the genetic distinctness of I pig with other Asian domestic pigs.
Highly efficient adsorption of arsenite from aqueous by zirconia modified activated carbon
Pham Ngoc Chuc,Nguyen Quang Bac,Dao Thi Phuong Thao,Nguyen Trung Kien,Nguyen Thi Ha Chi,Nguyen Van Noi,Vo Thang Nguyen,Nguyen Thi Hong Bich,Dao Ngoc Nhiem,Dinh Quang Khieu 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.2