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Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells
Gui-Hua Zhao,Lixin Zhang,Lisha Dai,Haozhi Xu,Chao Xu,Ting Xiao,Jin Li,Hui Sun,Beibei Zhou,Kun Yin 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.4
Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.
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Li, Hui,Wang, Beibei,Yang, Acong,Lu, Rui,Wang, Weicheng,Zhou, Yang,Shi, Guilai,Kwon, Sung Won,Zhao, Yingming,Jin, Ying Wiley (John WileySons) 2009 Stem Cells Vol.27 No.6
<P>Embryonic stem cells (ESCs) possess the capacity to self-renew and differentiate into all cell types of an organism. It is essential to understand how these properties are controlled for the potential usage of their derivatives in clinical settings and reprogramming of differentiated somatic cells. Although transcriptional factors, such as Oct4, Sox2, and Nanog, have been considered as a part of the core regulatory circuitry, a growing body of evidence suggests that additional factors exist and contribute to the control of ESC self-renewal and differentiation. Here, we report that Ly-1 antibody reactive clone (LYAR), a zinc finger nucleolar protein highly expressed in undifferentiated ESCs, plays a critical role in maintaining ESC identity. Its downregulation significantly reduces the rate of ESC growth and increases their apoptosis. Moreover, reduced expression of LYAR in ESCs impairs their differentiation capacity, failing to rapidly silence pluripotency markers and to activate differentiation genes upon differentiation. Mechanistically, LYAR forms a complex with another nucleolar protein, nucleolin, and prevents its self-cleavage, maintaining a normal steady-state level of nucleolin protein in undifferentiated ESCs. Interestingly, the downregulation of nucleolin is detrimental to the growth of ESCs and increases the rate of apoptosis, similarly to the knockdown of LYAR. Thus, our data emphasize the fact that other genes besides Oct4 and Nanog are uniquely required for ESC self-renewal and differentiation and demonstrate that LYAR functions to control the stability of nucleolin protein, which in turn is essential for maintaining the self-renewal of ESCs.</P>
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Acupuncture for Osteoporosis: a Review of Its Clinical and Preclinical Studies
Tian Yimiao,Wang Lili,Xu Tianshu,Li Rui,Zhu Ruyuan,Chen Beibei,Zhang Hao,Xia Bingke,Che Yiwen,Zhao Dandan,Zhang Dongwei 사단법인약침학회 2022 Journal of Acupuncture & Meridian Studies Vol.15 No.5
Acupuncture has gained growing attention in the management of osteoporosis (OP). However, a comprehensive review has not yet been conducted on the efficacy and challenges of acupuncture in preliminary research and clinical trials. Therefore, an extensive literature search was conducted using electronic databases, including PubMed (www.ncbi.nlm.nih.gov/pubmed), CNKI (www.cnki.net), and Web of Science, for studies published from the beginning of 2000 to the end of May 2022. Combinations of synonyms for OP, acupuncture, traditional Chinese medicine, clinical trial, preclinical study, and animal experiments were searched. A total of 290 papers were consulted, including 115 reviews, 109 clinical observations, and 66 preclinical studies. There is accumulating evidence to support the beneficial role of acupuncture in preserving bone quality and relieving clinical symptoms based on clinical and preclinical investigations. The top ten most commonly used acupoints are BL23, ST36, BL20, BL11, CV4, GV4, SP 6, KI3, BL18, and GB39. The underlying mechanisms behind the benefits of acupuncture may be linked with the regulation of the hypothalamic-pituitary-gonadal (adrenal) axis and activation of the Wnt/β-catenin and OPG/RANKL/RANK signaling pathways. In summary, strong evidence may still come from prospective and well-designed clinical trials to shed light on the potential role of acupuncture in preserving bone loss. Future investigations are needed to explore the potential underlying mechanisms, long-term clinical efficacy, and compliance of acupuncture in OP management.
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Yamin Hong,Xiupeng Yue,Yaping Zhang,Kai Shen,Wentao Jiao,Lei Zhao,Beibei Li 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.3
Sulfate radical-based advanced oxidation processes have been applied in the remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil, during which sulfate can be activated in different ways. In this work, effects of four different ways (FeSO4, NaOH, H2O2, and Heat) activated sodium persulfate (PS) on PAHs removal and soil physicochemical properties (pH, organic matter, functional groups, surface morphology, and partial heavy metal elements) were compared, and the influencing factors and mechanism of soil PAHs removal by thermally activated PS were also studied. The results showed that at the dosage of 3% of persulfate, the removal efficiency of PAHs followed the sequences of Heat (91.4%) > FeSO4 (86.6%) > H2O2 (86.2%) > NaOH (72.9%). However, thermal activation decreased the soil pH and organic matter content more significantly than other treatments. The reaction tended to reach equilibrium at 6 h when the dosage of persulfate was 3% and the activation temperature was 60 °C, and the reaction obeyed pseudo-first-order kinetics. Through quenching experiments, it was found that the free radicals playing a dominant role in the oxidation process were sulfate radicals. Compared with pH, liquid to soil (L/S ratio) and the temperature had more significant impacts on the degradation efficiency of PAHs.
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