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Ayse Erdem Yayla,Berrin Goktug Kadioglu,Ayse Aydin,Osman Aktas 대한산부인과학회 2019 Obstetrics & Gynecology Science Vol.62 No.4
ObjectiveHuman papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucousmembranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVsin married female patients with gynecological complaints, who had visited the Maternity Hospital in Erzurum, Turkey. MethodsIn this study, 263 cervical swab samples were taken from married women using the Pap smear method and wereinvestigated for positive reactivity against HPV. The L1 gene region of HPV was investigated using molecular methods. For this purpose, polymerase chain reaction (PCR) assays and sequence analysis of positive samples were performed. Phylogenetic analyses were performed using a bioinformatics approach after sequencing. ResultsHPV-DNA was detected in 17 (6.5%) samples. Highest positive reactivity to HPV-DNA was found in the 35–44 agegroup at 9.2%. Fourteen out of seventeen positive samples were included in the phylogenetic analysis. All isolatesclustered in the Alphapapillomavirus genus. Six samples were found to be HPV 70 positive, four were HPV 16 positive,and the rest were HPV 54, 72, 81, and 114 positive. When genotyping data were evaluated according to the riskgroup, we found that 28.6% of the 14 samples were found to be high risk-HPV, and 71.4% were low risk-HPV. ConclusionAs per our knowledge, this is the first report on the phylogenetic analysis of HPV genotypes isolated from women inTurkey. The prevalence of low- and-high risk HPV was determined in married women in Erzurum, and these resultscontribute to the epidemiological data on the distribution of HPV types for this region.