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- 검색키워드
- 저자 : Atif Ali Khan Khalil (검색결과 5 건)
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A new anti-Helicobacter pylori juglone from Reynoutria japonica
Atif Ali Khan Khalil,박우성,Jeehoon Lee,김혜진,Kazi Marjahan Akter,Young-Min Goo,Ji-Yeong Bae,Man-Seog Chun,Junghwan Kim,Mi-Jeong Ahn 대한약학회 2019 Archives of Pharmacal Research Vol.42 No.6
A 70% ethanol extract from the root portion of Reynoutria japonica afforded one new and three known juglone derivatives, namely, 2-methoxy-6-acetyl-7-methyljuglone (1), 2-ethoxy-6-acetyl-7-methyljuglone (2), 2-methoxy-7-acetonyljuglone (3), and 3-acetyl-7-methoxy-2-methyljuglone (4) together with two phenolics (5 and 6), an anthraquinone (7), a stilbene (8) and a phthalide (9). Their structures were elucidated on the basis of comprehensive spectroscopic studies including IR, MS, and 1H, 13C, 2D NMR spectra. Compound 3 is a new compound in nature, and compounds 4–6 have been isolated for the first time from R. japonica. The isolates were evaluated for their antibacterial activity against three strains (43504, 51, and 26695) of Helicobacter pylori. The four isolated juglone derivatives (1–4) showed potent growth inhibitory activity. Among them, compounds 1–3 exhibited stronger inhibitory activity than those of the positive controls, juglone and metronidazole, for the three strains and that of another reference, clarithromycin, for the 43504 and 51 strains. Specifically, the new juglone compound 3 displayed the most potent antibacterial activity against all three strains, 43504, 51, and 26695, with MIC values of 0.06, 0.06 and 0.13 μM, respectively, and MIC50 values of 0.14, 0.11 and 0.15 μM, respectively.
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Lupeol supplementation improves the developmental competence of bovine embryos <i>in vitro</i>
Khan, Imran,Chowdhury, M.M.R.,Song, Seok-Hwan,Mesalam, Ayman,Zhang, Shimin,Khan Khalil, Atif Ali,Jung, Eun-Hee,Kim, Ju-Boong,Jafri, Laila,Mirza, Bushra,Kong, Il-Keun Elsevier 2018 Theriogenology Vol.107 No.-
<P><B>Abstract</B></P> <P>Lupeol is a triterpene with various pharmacological properties. This study investigated the effect of lupeol on the <I>in vitro</I> development of bovine embryos. Oocytes <B>(</B>270 per group, 1620 in total<B>)</B> obtained from slaughterhouse-derived ovaries were matured and fertilized <I>in vitro</I> and then cultured for 8 days in a humidified atmosphere of 5% CO<SUB>2</SUB> in air at 38.5 °C. The <I>in vitro</I> maturation medium was supplemented with 0.5, 1.0, 2.0, 3.0, and 4.0 μM lupeol. Treatment with 2.0 μM lupeol significantly (<I>P</I> < 0.05) improved blastocyst development. Hoechst 33342 staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling showed that treatment with 2.0 μM lupeol improved blastocyst quality by increasing the total cell number and reducing the apoptotic cell number. Confocal microscopy confirmed that treatment with 2.0 μM lupeol significantly (<I>P</I> < 0.05) reduced the level of 8-oxoguanine, an indicator of reactive oxygen species. Lupeol treatment also significantly attenuated protein expression of nuclear factor-kappa B subunit 1 (NFKB1), cyclooxygenase (COX) 2, and CASP3. Real-time PCR analysis of <I>nitric oxide synthase 2</I>, <I>NFKB1</I>, <I>COX2</I>, <I>CASP3</I>, and <I>BCL2-associated X protein</I> supported the immunofluorescence data. In conclusion, lupeol is a potent antioxidant that improves bovine embryo development <I>in vitro</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lupeol is a potent antioxidant and it significantly improves the cleavage and blastocyst developmental potentials <I>in vitro</I>. </LI> <LI> Lupeol protects oocytes against oxidative stress in in <I>vitro</I>-culture system. </LI> <LI> Lupeol improves the embryo quality by increasing the total cell numbers and decreasing the apoptotic nuclei of embryo. </LI> <LI> Lupeol down regulates the NF-kB pathway and improves the bovine embryo quality <I>in vitro</I>. </LI> </UL> </P>
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Anti-Helicobacter pylori Activity of Compounds Isolated from Fraxinus mandshurica Bark
커지마자한악터,김혜진,박우성,Atif Ali Khan Khalil,안미정 한국생약학회 2020 Natural Product Sciences Vol.26 No.2
Helicobacter pylori is a well-known pathogen that is responsible for gastric disorders. Overcoming of the antibiotic-resistance is a main barrier to treat H. pylori infection. In our search for anti-H. pylori compounds from natural resources, bioactivity-guided isolation on the ethyl acetate fraction of Fraxinus mandshurica bark that had shown anti-H. pylori activity gave twelve compounds (1 - 12) of six coumarins, three phenylethanoids, two secoiridoids, and a lignan using silica gel column chromatography, Sephadex-LH 20, and recrystallization. The chemical structures were identified by spectroscopic data analysis, including 1D, 2D NMR, and mass spectrometry. Among them, compounds 2, 10, and 11 showed moderate growth inhibitory activity against three strains of H. pylori, compared with positive controls of quercetin and metronidazole. Compounds 5, 6, 8, and 12 exhibited the inhibitory activity against strains 26695 or 43504. This is the first report on the anti-H. pylori activity of this plant and the isolated compounds.
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Anatomical characterization and LC-MS profiling of <i>Adenophora</i> roots from Korea
Park, Woo Sung,Kim, Hye-Jin,Choe, Su Jin,Khalil, Atif Ali Khan,Akter, Kazi-Marjahan,Shin, Meong Cheol,Chung, Hye-Jin,Park, Jong Hee,Ahn, Mi-Jeong Elsevier 2019 Revista brasileira de farmacognosia Vol.29 No.6
<P><B>Abstract</B></P> <P>“Sasam (沙參)” is a crude drug that is defined in the in Korean Herbal Pharmacopoeia as the root of <I>Adenophora triphylla</I> var. <I>japonica</I> (Regel.) Hara or <I>A. stricta</I> Miq., Campanulaceae. The dried roots of the <I>Adenophora</I> spp. are available in markets, and the roots of various species are similar to each other in shape, making it difficult to distinguish one from another using only the outer morphological appearance. Therefore, the present study aimed to establish quality control parameters for pharmacognostic evaluation and differentiation of five <I>Adenophora</I> species and two varieties grown in Korea. Inner morphological evaluation of the root of these plants was accomplished and preliminary chemical analysis was performed by liquid chromatography-mass spectrometry profiling. As a result, significant differences among samples were found in anatomical characteristics such as number and thickness of cork layer, existence of stone cell in cork layer, frequency of vessels, and area of intercellular space. Significant differences were found among the samples in the content of three components including shashenoside I and a new alkyl glycoside, adenophoroside I. These findings could provide the scientific criteria for the proper identification and establishment of standards for the use of “Sasam”.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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