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Debprasad Chattopadhyay,Sonali Das,Asit Baran Mandal,,G Arunachalam,S K Bhattacharya 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.4
This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/ kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid, β-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.
Chattopadhyay, Debprasad,Das, Sonali,Mandal, Asit Baran,Arunachalam, G,Bhattacharya, SK Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.4
This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid, ${\beta}$-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.
Surianarayanan Mahadevan,Balaji Dhandapani,Senthilkumar Sivaprakasam,Asit Baran Mandal 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4
Biocalorimetry has proved to be an efficient tool for studying the energetics involved in several biochemical reactions. In this study, biocalorimetry was employed to simultaneously analyze biokinetics and bioenergetics involved during cultivation of a salt tolerant Pseudomonas aeruginosa for the production of alkaline protease. Batch experiments were performed in a bench scale biocalorimeter for alkaline protease production by P. aeruginosa using optimized process conditions. Tessier’s double substrate growth model was found to provide a good fit for the growth of P. aeruginosa in the biocalorimeter,and the biokinetic parameters were estimated. The heat flow profile resulting from metabolic activity of P. aeruginosa was shown to accurately depict both the kinetics of cell growth and protease production. Biokinetic and bioenergetic analysis on the growth of P. aeruginosa revealed that peptone is preferentially used as the substrate for its intracellular activities and glycerol acts as an energy source for its growth metabolism.
Chattopadhyay, Debprasad,Arunachalam, G.,Sur, T.K.,Bhattacharya, S.K.,Mandal, Asit B. Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.2
Two popular ethnomedicines of the Onge, a Negrito tribe of Andaman Islands, were evaluated for analgesic and antiinflammatory activity. The methanol extract as well as the different fractions of methanol extract of both Alstonia macrophylla and Mallotus peltatus leaves were studied using Swiss albino mice and Wistar albino rats. Acetic acid induced writhing, Tail flick and Tail immersion; Carrageenin- and Dextran-induced paw oedema tests were used. Dose-dependent analgesic and antiinflammatory activity were demonstrated for both methanol leaf extracts as well as fractions. Results were highly comparable with that of the standard drug pethidine.