Endocrine-specific NIR fluorophores for adrenal gland targeting

Ashitate, Yoshitomo,Levitz, Andrew,Park, Min Ho,Hyun, Hoon,Venugopal, Vivek,Park, GwangLi,El Fakhri, Georges,Henary, Maged,Gioux, Sylvain,Frangioni, John V.,Choi, Hak Soo The Royal Society of Chemistry 2016 Chemical communications Vol.52 No.67

<P>The adrenal glands (AGs) are relatively small yet require definitive identification during their resection, or more commonly their avoidance. To enable image-guided surgery involving the AGs, we have developed novel near-infrared (NIR) fluorophores that target the AGs after a single intravenous injection, which provided dual-NIR image-guided resection or avoidance of the AGs during both open and minimally-invasive surgery.</P>

Adrenal Gland Specific Near-Infrared Fluorophores for Image-Guided Surgery

Min Ho Park,Hoon Hyun,Yoshitomo Ashitate,Dang Huan Pham,Young Jae Ryu,Jin Seong Cho,Hak Soo Choi,Jung Han Yoon 대한외과학회 2016 대한외과학회 학술대회 초록집 Vol.2016 No.11

700-nm Zwitterionic Near-Infrared Fluorophores for Dual-Channel Image-Guided Surgery

Hyun, H.,Henary, M.,Gao, T.,Narayana, L.,Owens, E. A.,Lee, J. H.,Park, G.,Wada, H.,Ashitate, Y.,Frangioni, J. V. Elsevier Science B.V., Amsterdam. 2016 Molecular imaging and biology Vol.18 No.1

<P>Purpose: The purpose of this study was to develop a family of 700-nm zwitterionic pentamethine indocyanine near-infrared fluorophores that would permit dual-channel image-guided surgery. Procedures: Three complementary synthetic schemes were used to produce novel zwitterionic chemical structures. Physicochemical, optical, biodistribution, and clearance properties were compared to Cy5.5, a conventional pentamethine indocyanine now used for biomedical imaging. Results: ZW700-1a, ZW700-1b, and ZW700-1c were synthesized, purified, and analyzed extensively in vitro and in vivo. All molecules had extinction coefficients >= 199,000 M-1 cm(-1), emission >= 660 nm, and stability >= 99 % after 24 h in warm serum. In mice, rats, and pigs, >= 80 % of the injected dose was completely eliminated from the body via renal clearance within 4 h. Either alone or conjugated to a tumor targeting ligand, ZW700-1a permitted dual-channel, high SBR, and simultaneous imaging with 800-nm NIR fluorophores using the FLARE (R) imaging system. Conclusions: Novel 700-nm zwitterionic NIR fluorophores enable dual-NIR image-guided surgery.</P>

Reproductive Performance of Crossbred and Indigenous (Desi) Dairy Cows under Rural Context at Sirajgonj District of Bangladesh

Ashit Kumar Paul,Abdullah-Al-Maruf,Pankaj Kumar Jha,M. Golam Shahi Alam 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.4

This study was conducted among 120 different breed cows at selected areas of Sirajgonj district from March to July 2010 to compare the reproductive performance of crossbred and Desi cows at farmer’s level. The results showed that the average daily milk yield of Desi, Shahiwal x Desi, Friesian x Desi and Jersey x Desi cows was 2.3 ± 0.2, 4.9 ± 0.9, 6.0 ± 1.0 and 5.7 ± 0.9 liters, respectively. The milk yield of crossbred cows (5.5 ± 0.6 liters/day) was significantly (p<0.01) higher than Desi cows (2.3 ± 0.2 liters/day). The average age at puberty of Shahiwal x Desi, Friesian x Desi and Jersey x Desi was significantly (p<0.01) lower than that of Desi breed. The crossbred cows had significantly (p<0.01) lower pubertal age (20.4 ± 1.2) than Desi (25.9 ± 1.1). The age at first calving in Desi cows was significantly (p<0.01) higher (37.6 ± 1.1 months) than crossbred cows (31.2 ± 1.3 months). The average gestation length of Desi, Shahiwal x Desi, Friesian x Desi and Jersey x Desi was 289.9 ± 1.4, 285.0 ± 0.0, 285.0 ± 4.2 and 282.1 ± 2.4 days, respectively. It is suggested that the overall reproductive performance of Friesian x Desi, Jersey x Desi and Shahiwal x Desi cows were better than that of Desi cow.

Developing and Pre-Processing a Dataset using a Rhetorical Relation to Build a Question-Answering System based on an Unsupervised Learning Approach

Dutta, Ashit Kumar,Wahab sait, Abdul Rahaman,Keshta, Ismail Mohamed,Elhalles, Abheer International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.11

Rhetorical relations between two text fragments are essential information and support natural language processing applications such as Question - Answering (QA) system and automatic text summarization to produce an effective outcome. Question - Answering (QA) system facilitates users to retrieve a meaningful response. There is a demand for rhetorical relation based datasets to develop such a system to interpret and respond to user requests. There are a limited number of datasets for developing an Arabic QA system. Thus, there is a lack of an effective QA system in the Arabic language. Recent research works reveal that unsupervised learning can support the QA system to reply to users queries. In this study, researchers intend to develop a rhetorical relation based dataset for implementing unsupervised learning applications. A web crawler is developed to crawl Arabic content from the web. A discourse-annotated corpus is generated using the rhetorical structural theory. A Naïve Bayes based QA system is developed to evaluate the performance of datasets. The outcome shows that the performance of the QA system is improved with proposed dataset and able to answer user queries with an appropriate response. In addition, the results on fine-grained and coarse-grained relations reveal that the dataset is highly reliable.

Reproductive Performance of Crossbred and Indigenous (Desi) Dairy Cows under Rural Context at Sirajgonj District of Bangladesh

Paul, Ashit Kumar,Al-Maruf, Abdullah,Jha, Pankaj Kumar,Alam, M. Golam Shahi The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.4

This study was conducted among 120 different breed cows at selected areas of Sirajgonj district from March to July 2010 to compare the reproductive performance of crossbred and Desi cows at farmer's level. The results showed that the average daily milk yield of Desi, Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi cows was $2.3{\pm}0.2$, $4.9{\pm}0.9$, $6.0{\pm}1.0$ and $5.7{\pm}0.9$ liters, respectively. The milk yield of crossbred cows ($5.5{\pm}0.6$ liters/day) was significantly (p<0.01) higher than Desi cows ($2.3{\pm}0.2$ liters/day). The average age at puberty of Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi was significantly (p<0.01) lower than that of Desi breed. The crossbred cows had significantly (p<0.01) lower pubertal age ($20.4{\pm}1.2$) than Desi ($25.9{\pm}1.1$). The age at first calving in Desi cows was significantly (p<0.01) higher ($37.6{\pm}1.1$ months) than crossbred cows ($31.2{\pm}1.3$ months). The average gestation length of Desi, Shahiwal ${\times}$ Desi, Friesian ${\times}$ Desi and Jersey ${\times}$ Desi was $289.9{\pm}1.4$, $285.0{\pm}0.0$, $285.0{\pm}4.2$ and $282.1{\pm}2.4$ days, respectively. It is suggested that the overall reproductive performance of Friesian ${\times}$ Desi, Jersey ${\times}$ Desi and Shahiwal ${\times}$ Desi cows were better than that of Desi cow.

Use of Two Estrus Synchronization Protocols and Their Success on Pregnancy in Zebu and Crossbred Heifers at Char Areas of Bangladesh

Tapas Ghosh,Ashit Kumar Paul,Anup Kumar Talukder,M. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2012 한국동물생명공학회지 Vol.27 No.4

This study was designed to adopt two estrus synchronization protocols in zebu and crossbred heifers and their effects on pregnancy rate after timed artificial insemination (TAI). A number of 120 cyclic heifers were allotted for two different treatment groups and one control group. Heifers under protocol A were injected with GnRH at first day followed by a single dose of PGF2α at Day 11 and injection of GnRH at the day of AI; and heifers belonged to protocol B were treated with GnRH, two PGF_(2α) injections at 11 days apart and injection of GnRH at AI. AI was done at fixed time (within 72~96 hours after PGF_(2α) injection) in both protocols and pregnancy was confirmed by rectal palpation on 80~120 days of post AI. In control group; local heifers were conceived higher (30%) proportion than that of crossbred heifers (25%; p<0.05). In protocol A, the local breed were conceived higher (38.9%) proportion compared with crossbred (25%; p<0.05). In protocol B, local breed heifers were conceived higher (38.9%) proportion compared with crossbred heifers (33.3%; p<0.05). The overall pregnancy rate in protocol A and protocol B was 33.3% and 36.6%, respectively. The proportion of pregnancy rate of local heifers (38.9%; Protocol A) was significant (p<0.05) in comparison with local heifers (30%) in control group (p<0.05). The overall pregnancy rate between pooled control group (28.3%) and treatment group (35%) was significantly (p<0.05) differ from each other’s. Results of present study concluded that estrus synchronization followed by fixed time AI could be applied for higher pregnancy rate in zebu and crossbred heifers

Improvement of Preservation Quality of Chilled Bull Semen Using ${\alpha}$-tocopherol as an Antioxidant

Jha, Pankaj Kumar,Paul, Ashit Kumar,Rahman, M. Bozlur,Tanjim, M.,Bari, Farida Yeasmin,Alam, M. Golam Shahi The Korean Society of Embryo Transfer 2013 한국동물생명공학회지 Vol.28 No.1

Alpha-tocopherol as an antioxidant acts in preservation of chilled semen by preserving cell membrane damage from lipid peroxidation. Optimum concentrations of ${\alpha}$-tocopherol in egg yolk-citrate (EYC) extender need to be studied in crossbred bull's semen. Different concentrations of ${\alpha}$-tocopherol viz. 0, 1, 2, 4 and 6mg per ml of extender were used. Semen was collected once a week from four bulls used to regular collection, aged 4 to 7 years, weighing 320 to 450 kg, and with body condition score 4 to 4.5 and scrotal circumference 23 to 32 cm. Semen was evaluated routinely and sperm morphology was viewed under light microscope at ${\times}1,000$ magnification after fixing with buffered formal saline. Over 90% had normal head, acrosome, mid-piece and tail. Semen was diluted with egg-yolk-citrate extender to produce $15{\times}10^6$ spermatozoa/ml and 0, 1, 2, 4 and 6 mg/ml ${\alpha}$-tocopherol were added. The semens amples were kept at $8^{\circ}C$. Sperm motility and viability were examined daily up to 5 days under light microscopy at ${\times}200$ magnification. Sperm viability was acceptable (${\geq}40%$) up to the $4^{th}$ day with all concentrations of ${\alpha}$-tocopherol and up to the $5^{th}$ day with 2 mg/ml ${\alpha}$-tocopherol. Sperm motility was acceptable (${\geq}40%$) up to the $3^{rd}$ day irrespective of ${\alpha}$-tocopherol concentration, and up to the $4^{th}$ day with 2 mg/ml ${\alpha}$-tocopherol. It is suggested that the lifespan of chilled semen may be extended up to 4 days by adding 2mg/ml ${\alpha}$-tocopherol.

Use of Two Estrus Synchronization Protocols and Their Success on Pregnancy in Zebu and Crossbred Heifers at Char Areas of Bangladesh

Ghosh, Tapas,Paul, Ashit Kumar,Talukder, Anup Kumar,Alam, M. Golam Shahi,Bari, Farida Yeasmin 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.4

This study was designed to adopt two estrus synchronization protocols in zebu and crossbred heifers and their effects on pregnancy rate after timed artificial insemination (TAI). A number of 120 cyclic heifers were allotted for two different treatment groups and one control group. Heifers under protocol A were injected with GnRH at first day followed by a single dose of at Day 11 and injection of GnRH at the day of AI; and heifers belonged to protocol B were treated with GnRH, two injections at 11 days apart and injection of GnRH at AI. AI was done at fixed time (within 72~96 hours after injection) in both protocols and pregnancy was confirmed by rectal palpation on 80~120 days of post AI. In control group; local heifers were conceived higher (30%) proportion than that of crossbred heifers (25%; p<0.05). In protocol A, the local breed were conceived higher (38.9%) proportion compared with crossbred (25%; p<0.05). In protocol B, local breed heifers were conceived higher (38.9%) proportion compared with crossbred heifers (33.3%; p<0.05). The overall pregnancy rate in protocol A and protocol B was 33.3% and 36.6%, respectively. The proportion of pregnancy rate of local heifers (38.9%; Protocol A) was significant (p<0.05) in comparison with local heifers (30%) in control group (p<0.05). The overall pregnancy rate between pooled control group (28.3%) and treatment group (35%) was significantly (p<0.05) differ from each other's. Results of present study concluded that estrus synchronization followed by fixed time AI could be applied for higher pregnancy rate in zebu and crossbred heifers.

Epidemiological Investigation and Antibiotic Sensitivity of Salmonellosis in Goats at the Selected Areas of Bangladesh

Gobindha Kumar Saha,Ashit Kumar Paul,Abdussamad,M. Ariful Islam,M. Shahidur Rahman Khan 사단법인 한국동물생명공학회 2013 한국동물생명공학회지 Vol.28 No.4

Salmonellosis is one of the life-threating diseases of goat in Bangladesh. Therefore, the present study wasdesigned to study the prevalence of Salmonellosis, and isolation and characterizations of the Salmonella spp. fromapparently healthy and diarrheic goat. A total of 47 faces samples were collected from selected place and culturedonto different prescribed medium to isolate it. In this study, 12.76% (6/47) samples were found to be positive forSalmonella spp. During culture on SS agar medium, all of the Salmonella isolates produced round, smooth, opaque,translucent and black color colonies on SS agar media. All of the isolated Salmonella spp. fermented dextrose,maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. However, theseisolates had showed Indole and Voges-Proskauer test negative, Methyl-Red test positive. All of these isolates weresubjected to rapid plate agglutination test with polyvalent “O” (Poly ‘O’) and polyvalent “H” (poly ‘H’) antiserawhere positive agglutination were observed. They were highly sensitive to ciprofloxacin, spiramycin and gentamycin;moderately sensitive to oxytetracyline, streptomycin and amoxicillin; less sensitive to sulphamethoxazole and resistantto penicillin-G. Based on the present findings, it may be concluded that the investigated Salmonella spp. from goatsmight be S. typhimurium, S. enteritidis, S. brandenburg, S. salford, S. newbrunswick, S. newport or S. dublin. Furtherstudy will be needed, therefore it requires further characterization using other serological and molecular techniques.