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- 저자 : Ashish Tripathi (검색결과 4 건)
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Herbivore-responsive cotton phenolics and their impact on insect performance and biochemistry
Garima Dixit,Ashish Praveen,Tusha Tripathi,Vikash Kumar Yadav,Praveen C. Verma 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Insect damage to plants can have several impacts on plant biochemical composition and such changes may influence the insect performances. Here,we examined herbivore-responsive alterations in the phenolic profile of cotton plant using three cotton genotypes, Bc-68-2, Gcot-16 and Coker-312, belonging to G. hirsutumsp. during their infestation with two biting and chewing insects viz., Helicoverpa armigera and Spodoptera litura, separately for 96 h. Both insects had similar effects on phenolic induction in cotton plants (Gossypium sp.) at 96 h post infestation (hpi) however, the phenolic accumulationwas genotype specific.Wemeasured 13 phenolic compounds, out ofwhich four phenolic acids viz., gallic acid (GA), cinnamic acid (CA), p-coumaric acid (PA) and salicylic acid (SA) displayed higher accumulation in response to insect herbivory at 96 hpi. The subsequent impact of four herbivore- responsive phenolics on insect performance was evaluated through diet incorporation assay. Insect larvae displayed significant reduction in larvalweights and substantial mortality on 10th day in case of CA and PA incorporated diets at higher concentrations. Physiological examination of treated larvae on 5th and 10th day post feeding at CA and PA containing diets revealed oxidative stress, reduced lactate dehydrogenase enzyme activity, altered glucose and protein level and enhanced glutathione-S-transferase enzyme activity in larvae feeding on treatment diets as compared to controls.Our results suggest that the defensive chemistry of phenolics has important effect on the insect fitness and physiology and the flavonoids such as CA and PA can be utilized in pest control programmes.
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Pushpendra Singh,Manish Kumar Tripathi,Mohammad Yasir,Ashish Ranjan,Rahul Shrivastava 환경독성보건학회 2021 환경독성보건학회지 Vol.36 No.3
Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ΔG -4.11 kcal/mole and ΔG -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.
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Yakhari Kashung,Biswajit Das,Sangeeta Deka,Reetashree Bordoloi,Ashish Paul,Om Prakash Tripathi 한국산림과학회 2018 Forest Science And Technology Vol.14 No.2
Comprehending the prominence of forest carbon in climate change, this study was piloted in different land use of West Kameng district, Arunachal Pradesh, India to record the floristic composition, community characteristics, and above ground biomass (AGB) carbon using random sampling and geospatial approach. Preliminary field survey was done in 2016. Altogether 45 quadrats (0.1 ha each) were laid. Total tree richness recorded was 164 species from 49 families. Dominance and frequency distribution pattern of species revealed heterogeneity in composition with majority species showing clumped distribution. Plantations showed highest tree density while mixed dense forest showed maximum basal area (58.89 m2 ha¡1). Estimated AGB were 218.21 ton/ha for mixed dense forest, 84.94 ton/ha for abandoned forest, and 105.09 ton/ha for plantations. Total estimated carbon stocks were 120.01, 46.17, and 57.80 ton/ha for mixed dense, abandoned forest, and plantations, respectively. Predicted average AGB using Geographic Information System (GIS) techniques was 163.25 ton/ha. Field-based AGB was slightly greater than the values observed from satellite data. Findings of the study may be useful for calculating total biomass and carbon stored in the major land cover of the district in particular and region in general. It will also support in future studies for calculating the long-term data on biomass carbon sequestration.
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Arshdeep Singh,Vandana Midha,Vikram Narang,Saurabh Kedia,Ramit Mahajan,Pavan Dhoble,Bhavjeet Kaur Kahlon,Ashvin Singh Dhaliwal,Ashish Tripathi,Shivam Kalra,Narender Pal Jain,Namita Bansal,Rupa Banerje 대한장연구학회 2023 Intestinal Research Vol.21 No.4
Background/Aims: Primary sclerosing cholangitis (PSC) represents the most common hepatobiliary extraintestinal manifestation of inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s disease (CD). Limited data exist on PSC in patients with IBD from India. We aimed to assess the prevalence and disease spectrum of PSC in Indian patients with IBD. Methods: Database of IBD patients at 5 tertiary care IBD centers in India were analyzed retrospectively. Data were extracted and the prevalence of PSC-IBD was calculated. Results: Forty-eight patients out of 12,216 patients with IBD (9,231 UC, 2,939 CD, and 46 IBD unclassified) were identified to have PSC, resulting in a prevalence of 0.39%. The UC to CD ratio was 7:1. Male sex and pancolitis (UC) or colonic CD were more commonly associated with PSC-IBD. The diagnosis of IBD preceded the diagnosis of PSC in most of the patients. Majority of the patients were symptomatic for liver disease at diagnosis. Eight patients (16.66%) developed cirrhosis, 5 patients (10.41%), all UC, developed malignancies (3 colorectal cancer [6.25%] and 2 cholangiocarcinoma [4.16%]), and 3 patients died (2 decompensated liver disease [4.16%] and 1 cholangiocarcinoma [2.08%]) on follow-up. None of the patients mandated surgical therapy for IBD. Conclusions: Concomitant PSC in patients with IBD is uncommon in India and is associated with lower rates of development of malignancies.
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