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RISS 인기검색어
- 검색키워드
- 저자 : Arum Han (검색결과 27 건)
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Microsystems for Whole Blood Purification and Electrophysiological Analysis
Arum Han,Ki-Ho Han,Swomitra K. Mohanty,A. Bruno Frazier 대한전자공학회 2005 Journal of semiconductor technology and science Vol.5 No.1
This paper presents the development of a microsystem for whole blood purification and electrophysiological analysis of the purified cells. Magnetophoresis using continuous diamagnetic capture (DMC) was utilized for whole cell purification and electrical impedance spectroscopy (EIS) was utilized for electrophysiological analysis of the purified cells. The system was developed on silicon and plastic substrates utilizing conventional microfabrication technologies and plastic microfabrication technologies. Using the magnetophoretic microseparator, white blood cells were purified from a sample of whole blood. The experimental results of the DMC microseparator show that 89.7% of the red blood cells (RBCs) and 72.7% of the white blood cells (WBCs) could be continuously separated out from a whole blood using an external magnetic flux of 0.2 T. EIS was used as a downstream whole cell analysis tool to study the electrophysiological characteristics of purified cells. In this work, primary cultured bovine chromaffin cells and human red blood cells were characterized using EIS. Further analysis capabilities of the EIS were demonstrated by successfully obtaining unique impedance signatures for chromaffin cells based on the whole cell ion channel activity.
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Microsystems for Whole Blood Purification and Electrophysiological Analysis
Han, Arum,Han, Ki-Ho,Mohanty Swomitra K.,Frazier A. Bruno The Institute of Electronics and Information Engin 2005 Journal of semiconductor technology and science Vol.5 No.1
This paper presents the development of a microsystem for whole blood purification and electrophysiological analysis of the purified cells. Magnetophoresis using continuous diamagnetic capture (DMC) was utilized for whole cell purification and electrical impedance spectroscopy (EIS) was utilized for electrophysiological analysis of the purified cells. The system was developed on silicon and plastic substrates utilizing conventional microfabrication technologies and plastic microfabrication technologies. Using the magnetophoretic microseparator, white blood cells were purified from a sample of whole blood. The experimental results of the DMC microseparator show that 89.7% of the red blood cells (RBCs) and 72.7% of the white blood cells (WBCs) could be continuously separated out from a whole blood using an external magnetic flux of 0.2 T. EIS was used as a downstream whole cell analysis tool to study the electrophysiological characteristics of purified cells. In this work, primary cultured bovine chromaffin cells and human red blood cells were characterized using EIS. Further analysis capabilities of the EIS were demonstrated by successfully obtaining unique impedance signatures for chromaffin cells based on the whole cell ion channel activity.
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A disposable microfluidic flow sensor with a reusable sensing substrate
Kim, Jinho,Cho, Hyungseok,Han, Song-I,Han, Arum,Han, Ki-Ho Elsevier 2019 Sensors and actuators. B, Chemical Vol.288 No.-
<P><B>Abstract</B></P> <P>In many biological, medical and pharmaceutical applications, disposable flow sensors are highly desired to prevent biological contamination. However, due to their high cost, conventional microfluidic flow sensors are difficult to be used as disposable. This paper introduces a disposable microfluidic flow sensor (disposable-μFSensor) consisting of a disposable microchannel superstrate and reusable sensing substrate, which can be assembled and disassembled simply by vacuum pressure. Because the disposable microchannel superstrate is fabricated easily and cheaply using a silicone-coated release polymer thin film, it can be replaced after a single use, while the sensing substrate can be reused continuously. Experimental results show that the sensitivity, reproducibility, response time, long-term stability, resolution, and accuracy of the disposable-μFSensor are comparable to those of commercial microfluidic flow sensors. One of the key advantages of the disposable-μFSensor is that the measurable flow rate range can be adjusted very broadly, from 10 to 650 μL/min, by simply replacing only the disposable microchannel that has different bypass channel design, without having to change the sensing substrate. To demonstrate the usefulness of the disposable-μFSensor for biological, medical and pharmaceutical applications, the developed sensor was used to control the flow rates of a droplet generator, with the generated droplets having variations in diameter and volume of less than 1.25% and 4%, respectively. In summary, we report that the disposable-μFSensor is reproducible and accurate for various applications at low cost as well as resistant to biological contamination, in contrast to conventional flow sensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A disposable microfluidic flow sensor for preventing biological contamination. </LI> <LI> Hybrid flow sensor using a disposable microchannel and a reusable sensing substrate. </LI> <LI> Disposable microchannel made of a PET thin film; simple fabrication and low cost. </LI> <LI> Performance comparable to that of commercial flow sensors. </LI> <LI> Adjustable full-scale flow rate by replacing disposable microchannel without changing substrate. </LI> </UL> </P>
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Three pediatric patients with primary hyperparathyroidism caused by parathyroid adenoma
Oh Arum,Lee Yena,Yoo Han-Wook,Choi Jin-Ho 대한소아내분비학회 2022 Apem Vol.27 No.2
Primary hyperparathyroidism (PHPT) is a hypercalcemia disorder with inappropriately normal or increased serum parathyroid hormone (PTH) levels resulting from excessive secretion of PTH from one or more of the parathyroid glands. PHPT is uncommon in infants and children, with an estimated incidence of 2–5 cases per 100,000 persons. Patients with PHPT usually present with bone pain, urolithiasis, or nephrolithiasis, as well as nonspecific symptoms such as fatigue and weakness. Asymptomatic hypercalcemia may also be detected incidentally. Only a few cases of pediatric PHPT have been reported in Korea. We present three patients (a 9-year-old girl, a 14-year-old boy, and a 14-year-old girl) with PHPT who manifested variable clinical features of hypercalcemia. The first and second patients each had a parathyroid adenoma and presented with abdominal pain caused by pancreatitis and a ureter stone, respectively. The third patient had an ectopic mediastinal parathyroid adenoma and presented with gait disturbance and weakness of the lower extremities. All of the patients underwent surgical resection of parathyroid adenoma, and their serum calcium levels subsequently normalized without medication.
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Three pediatric patients with primary hyperparathyroidism caused by parathyroid adenoma
Oh Arum,Lee Yena,Yoo Han-Wook,최진호 대한소아내분비학회 2022 Annals of Pediatirc Endocrinology & Metabolism Vol.27 No.2
Primary hyperparathyroidism (PHPT) is a hypercalcemia disorder with inappropriately normal or increased serum parathyroid hormone (PTH) levels resulting from excessive secretion of PTH from one or more of the parathyroid glands. PHPT is uncommon in infants and children, with an estimated incidence of 2–5 cases per 100,000 persons. Patients with PHPT usually present with bone pain, urolithiasis, or nephrolithiasis, as well as nonspecific symptoms such as fatigue and weakness. Asymptomatic hypercalcemia may also be detected incidentally. Only a few cases of pediatric PHPT have been reported in Korea. We present three patients (a 9-year-old girl, a 14-year-old boy, and a 14-year-old girl) with PHPT who manifested variable clinical features of hypercalcemia. The first and second patients each had a parathyroid adenoma and presented with abdominal pain caused by pancreatitis and a ureter stone, respectively. The third patient had an ectopic mediastinal parathyroid adenoma and presented with gait disturbance and weakness of the lower extremities. All of the patients underwent surgical resection of parathyroid adenoma, and their serum calcium levels subsequently normalized without medication.
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Lee Yena,최진호,Oh Arum,Kim Gu-Hwan,Park Sook-Hyun,Moon Jung Eun,Ko Cheol Woo,Cheon Chong-Kun,Yoo Han-Wook 대한소아내분비학회 2020 Annals of Pediatirc Endocrinology & Metabolism Vol.25 No.2
Purpose: Cytochrome P450 oxidoreductase (POR) deficiency is a rare autosomal recessive disorder caused by mutations in the POR gene encoding an electron donor for all microsomal P450 enzymes. It is characterized by adrenal insufficiency, ambiguous genitalia, maternal virilization during pregnancy, and skeletal dysplasia. In this study, we investigated the clinical, hormonal, and molecular characteristics of patients with POR deficiency in Korea. Methods: This study included four patients with POR deficiency confirmed by biochemical and molecular analysis of POR. Clinical and biochemical findings were reviewed retrospectively. Mutation analysis of POR was performed by Sanger sequencing after polymerase chain reaction amplification of all coding exons and the exon-intron boundaries. Results: All patients presented with adrenal insufficiency and ambiguous genitalia regardless of their genetic sex. Two patients harbored homozygous p.R457H mutations in POR and presented with adrenal insufficiency and genital ambiguity without skeletal phenotypes. The other two patients with compound heterozygous mutations of c.[1329_1330insC];[1370G>A] (p.[I444Hfs*6];[R457H]) manifested skeletal abnormalities, such as craniosynostosis and radiohumeral synostosis, suggesting Antley-Bixler syndrome. They also had multiple congenital anomalies involving heart, kidney, and hearing ability. All patients were treated with physiologic doses of oral hydrocortisone. Conclusion: We report the cases of 4 patients with POR deficiency identified by mutation analysis of POR. Although the study involved a small number of patients, the POR p.R457H mutation was the most common, suggesting founder effect in Korea. POR deficiency is rare and can be misdiagnosed as 21-hydroxylase or 17α-hydroxylase/17,20-lyase deficiency. Therefore, molecular analysis is critical for confirmatory diagnosis.
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An ANKRD11 exonic deletion accompanied by a congenital megacolon in an infant with KBG syndrome
Go Hun Seo,Arum Oh,Minji Kang,Eun Na Kim,Ja-Hyun Jang,Dae Yeon Kim,Kyung Mo Kim,Han-Wook Yoo,Beom Hee Lee 대한의학유전학회 2019 대한의학유전학회지 Vol.16 No.1
KBG syndrome is an autosomal dominant syndrome presenting with macrodontia, distinctive facial features, skeletal anoma-lies, and neurological problems caused by mutations in the ankyrin repeat domain 11 (ANKRD11) gene. The diagnosis of KBG is difἀcult in very young infants as the characteristic macrodontia and typical facial features are not obvious. The youngest patient diagnosed to date was almost one year of age. We here describe a 2-month-old Korean boy with distinctive craniofa-cial features but without any evidence of macrodontia due to his very early age. He also had a congenital megacolon without ganglion cells in the rectum. A de novo deletion of exons 5-9 of the ANKRD11 gene was identiἀed in this patient by exome sequencing and real-time genomic polymerase chain reaction. As ANKRD11 is involved in the development of myenteric plexus, a bowel movement disorder including a congenital megacolon is not surprising in a patient with KBG syndrome and has possibly been overlooked in past cases.
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