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Sadashiv,Anupama Modi,Manoj Khokhar,Praveen Sharma,Rajnish Joshi,Sudhanshu Shekhar Mishra,Rajay N Bharshankar,Sunita Tiwari,Pankaj Kumar Singh,Vivek Vidyadhar Bhosale,Mahendra Pal Singh Negi 대한비만학회 2021 The Korean journal of obesity Vol.30 No.3
Background: It is well established that obesity is a major health risk in diabetes and associated diseases. Epigenetic changes, specially DNA methylation, play an important role in regulation of adipokines. The objective of the present study was to evaluate the DNA methylation status at the promoter region of the leptin gene in obese individuals and its association with metabolic risk factors. Methods: The study included obese (n=100) and non-obese (n=75) individuals aged 25–45 years, and measured their physical, biochemical parameters (glucose, insulin, and lipid profiles) and leptin, DNA methyltransferase 1 (DNMT1), and DNA methyltransferase 3 beta (DNMT3b) mRNA expressions with real-time reverse transcription-polymerase chain reaction (qRT-PCR). DNA methylation of the leptin gene at the promoter region was analyzed by methyl-specific qPCR . Results: The study found that the DNA methylation level at the promoter area of the leptin gene was negatively associated with weight in obese subjects. Furthermore, study findings showed that the DNA methylation level was negatively associated with fasting insulin, glucose, homeostatic model assessment for insulin resistance, and total cholesterol. There was also a higher expression of DNMT1 and DNMT-3b in obese subjects as compared with non-obese subjects. Conclusion: The leptin epigenetic profile may be associated with obesity and its associated metabolic risk factors.
Shweta Suri,Anupama Singh,Prabhat K. Nema 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.13
Citrus fruits are well known for their medicinaland therapeutic potential due to the presence of immensebioactive components. With the enormous consumption ofcitrus juice, citrus processing industries are focused on theproduction of juice but at the same time, a large amount ofwaste is produced mainly in the form of peel, seeds,pomace, and wastewater. This waste left after processingleads to environmental pollution and health-related hazards. However, it could be exploited for the recovery ofessential oils, pectin, nutraceuticals, macro and micronutrients,ethanol, and biofuel generation. In view of theimportance and health benefits of bioactive compoundsfound in citrus waste, the present review summarizes therecent work done on the citrus fruit waste valorization forrecovery of value-added compounds leading to zerowastage. Therefore, instead of calling it waste, these couldbe a good resource of significant valuable components, inthis way encouraging the zero-waste theory.
Recent advances in CRISPR/Cas mediated genome editing for crop improvement
Samriti Sharma,Rajinder Kaur,Anupama Singh 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.4
Plant breeding is one of the oldest sustainable agriculture methods used to increase the yield, quality and other biomaterial for human use. Many crops like fruits, vegetables, ornamental flowers, shrubs and trees, have been long cultivated to satisfy human food and aesthetical needs. Conventional breeding technologies like selection, hybridization, mutation through physical and chemical methods, and modern transgenic approaches are often used to improve the desired traits without inducing the pleiotropic effects. But these breeding methods are highly laborious and complicated to enhance crop production. Recently, targeted genome editing through engineered nuclease including zinc finger nuclease, transcription activator like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPRs) have been used to improve various traits in plants. Genome editing has emerged as a novel alternative approach to classical breeding with higher mutagenic efficiency. Here, we briefly cover the strengths of CRISPRs in comparison with other genome editing techniques. We also discuss its potential applications in genetic improvement of various crops and future prospective.
Joshi, Akshay,Vasudevan, Gowdaman,Engineer, Anupama,Pore, Soham,Hivarkar, Sai Suresh,Lanjekar, Vikram Bholanath,Dhakephalkar, Prashant Kamalakar,Dagar, Sumit Singh The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.1
A xylanolytic and cellulolytic anaerobic bacterium strain CtC72 was isolated from cattle rumen liquor. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain CtC72 shared only 97.78% homology with its nearest phylogenetic affiliate Actinomyces ruminicola, showing its novelty. The strain could grow on medium containing xylan, carboxymethyl cellulose and avicel producing $CO_2$, acetate, and ethanol as major fermentation products. The whole genome analysis of the strain CtC72 exhibited a broad range of carbohydrate-active enzymes required for the breakdown and utilization of lignocellulosic biomass. Genes related to the production of ethanol and stress tolerance were also detected. Further there were several unique genes in CtC72 for chitin degradation, pectin utilization, sugar utilization, and stress response in comparison with Actinomyces ruminicola. The results show that the strain CtC72, a putative novel bacterium can be used for lignocellulosic biomass based biotechnological applications.
Manonmani Thiruvalluvan,Barjinder Pal Kaur,Anupama Singh,Sanjana Kumari 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.2
Fruits and vegetables are one of the most consumed and processed commodities globally and comprise abundant phenolic compounds, one of the main nutraceuticals in the food industry. Comparably elevated rates of these compounds are found in waste (peel, seeds, leaf, stem, etc.) in the food processing industry. They are being investigated for their potential use in functional foods. However, phenolic compounds’ low bioavailability limits their application, which can be approached by loading the phenolic compounds into an encapsulation system such as liposomal carriers. This review aims to elucidate the recent trend in extracting phenolic compounds from the waste stream and the means to load them in stable liposomes. Furthermore, the application of these liposomes with only natural extracts in food matrices is also presented. Many studies have indicated that liposomes can be a proper candidate for encapsulating and delivering phenolic compounds and as a means to increase their bioavailability.