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Mycotoxins’ evaluation in wheat flours used in Brazilian bakeries
Andressa Lanza,Rosselei Caie´l da Silva,Ingrid Duarte dos Santos,Ionara Regina Pizzutti,Karine Cence,Roge´rio Luis Cansian,Jamile Zeni,Eunice Valduga 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
The aim of this study was to evaluate themycotoxicological quality of wheat flours used by bakeriesfrom the North Region in Rio Grande do Sul state, Brazil,regarding the presence of mycotoxins. On collecting type-1refined wheat flour, a conglomerate sampling from 13 citiesand 3 bakeries per city (n = 39), selected from the definedregion was performed. The mycotoxins analysis was usingQuEChERS method and UPLC–MS/MS analysis. As aresult, 100% of samples presented contamination by DON,with concentrations ranging from 76.7 to 3630.2 lg kg-1and ZON was found in one sample (26.7 lg kg-1), whichrepresented 2.6% of the analyzed wheat flours. Othermycotoxins (AFB1, AFB2, AFG1, AFG2, DAS, HT-2toxin, OTA, FB1 and FB2) were not detected in the analyzedsamples.
Light Quality on the In Vitro Growth and Production of Pigments in the Genus Alternanthera
Andressa Reis,Alitcia Moraes Kleinowski,Fátima Rosane Schuquel Klein,Renata Trevizan Telles,Luciano do Amarante,Eugenia Jacira Bolacel Braga 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.5
In tissue culture, several chemical and physiological factors can influence the production of secondary metabolites. The growth response and increased secondary metabolite production generated by a high irradiance environment, can be used to determine a favorable habitat for the growth and conservation of medicinal plants. Thus, the present study aimed to evaluate the influence of light quality on growth and production of secondary metabolites in Alternanthera sessilis (L.) R. Br. ex DC. (sessile joyweed), Alternanthera philoxeroides (Mart.) Grisebach (alligatorweed), Alternanthera tenella Colla (joyweed), and Alternanthera brasiliana (L.) Kuntze (Brazilian joyweed) plants cultured in vitro. The species were grown in MS medium, for 45 days in different light qualities (blue, white, and red). Growth parameters and biochemical analysis were performed at the end of the study. The results show that the red light allowed a higher accumulation of biomass in most species; red and white light were great inductors for the production of betacyanin and the blue light favored the enhancement of flavonoids. In this manner, the quality of light can affect the betacyanin, betaxanthin, and flavonoid biosynthesis as well as growth patterns in the related Alternanthera species. Based on the data presented, we suggest that specific genes can encode the pigment production according to the light quality which exhibit distinct activation patterns in different plant species.
Bonacina Cristine,Nascimento Andressa Bezerra,Barbosa Letícia Neris,de Souza Silvia Graciele Hülse,Magalhães Hélida Mara 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2
During cultivation, environmental factors can cause changes in the metabolism of Curcuma longa L. that limit its development. This study evaluated the efects of diferent sodium chloride (NaCl) concentrations on the leaf anatomy, stomatal features, and biochemical activity of C. longa L. plants. The experiment was conducted in a greenhouse, using rhizomes of C. longa L. planted in plastic pots containing soil, sand, and organic matter (2:1:1). After 180 days of planting, salt stress using NaCl (50, 100, and 150 mM) was induced and a control treatment was made with water. At 150 days post treatment, the morphoanatomical variables of the leaves were evaluated, and biochemical analyses of the leaves and rhizomes were performed. The leaves exposed to intermediate salt concentrations (50 and 100 mM) had fewer hypodermic cells and larger spaces between the cells in the spongy parenchyma. In the 150 mM NaCl group, there was an increase in the total leaf thickness caused by an increase in hypodermic cells. The percentage of closed stomata increased in the 50 mM (85%) and 100 mM (80%) NaCl treatments, and the polar diameter of the cells (1.22%) was reduced in the presence of 150 mM NaCl. Biochemical analyses revealed diferent responses for rhizomes and leaves. The total sugars in the rhizomes increased in the presence of ≥50 mM NaCl, probably because of the need to generate more energy for use in secondary metabolite synthesis pathways. The antioxidative activity and phenolic compounds also increased at the highest NaCl concentrations. In conclusion, we have classifed C. longa L. as a plant that is tolerant to saline stress, owing to the metabolic balance and water-loss barrier provided by the rhizome to mitigate stress damage. Our results showed that C. longa L. plants were tolerant to the high salt concentrations tested in this study (100 and 150 mM).
Larissa Akemi Kido,Isabela Maria Urra Rossetto,Andressa Mara Baseggio,Gabriela Bortolanza Chiarotto,Letícia Ferreira Alves,Felipe Rabelo Santos,Celina de Almeida Lamas,Mário Roberto Maróstica Jr,Valér 대한암예방학회 2022 Journal of cancer prevention Vol.27 No.3
Jaboticaba is a Brazilian berry, which is rich in fibers and bioactive compounds and shows high antioxidant and antiproliferative activities. Prostate cancer (PCa) is the second most common type of cancer among men and its progression is influenced by androgens and inflammation. Previous studies reported the ability of the jaboticaba to modulate pathways involved in prostate diseases. The main objective of this study was to provide significant data about molecular targets of the jaboticaba peel extract (JPE) and its mechanisms of action in PCa cell lines with different androgenic status (LNCaP and PC-3). The results showed that JPE was able to decrease cell viability in both cell lines. LNCaP showed more sensitivity to JPE exposure, indicating the efficacy of the JPE treatment in terms of androgen responsiveness. JPE showed a distinct hormone dependent effect on the NF-κB signaling, with reduced NF-κB levels for LNCaP and increased NF-κB levels in PC-3 cells. Mechanisms related to cell death by apoptosis were stimulated after the JPE treatment, modulating B-cell lymphoma 2 and BAX for LNCaP and PC-3. Particularly for PC-3, the JPE treatment resulted in cytokine-cytokine receptor interaction activation mostly by up regulating pro-inflammatory, pro-angiogenic, immunostimulatory and immunosuppressive genes. Also, a set of genes related to angiogenesis and metastasis were down-regulated by JPE. In conclusion, JPE exerted an antitumor effect on PCa for both cell lines which can be enhanced if androgenic reliance is considered.
Poletto, Daniel,Poletto, Ana Claudia,Cavalaro, Andressa,Machado, Ricardo,Cosme-Silva, Leopoldo,Garbelini, Cassia Cilene Dezan,Hoeppner, Marcio Grama The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.4
Objectives: This study evaluated smear layer removal by different chemical solutions used with or without ultrasonic activation after post preparation. Materials and Methods: Forty-five extracted uniradicular human mandibular premolars with single canals were treated endodontically. The cervical and middle thirds of the fillings were then removed, and the specimens were divided into 9 groups: G1, saline solution (NaCl); G2, 2.5% sodium hypochlorite (NaOCl); G3, 2% chlorhexidine (CHX); G4, 11.5% polyacrylic acid (PAA); G5, 17% ethylenediaminetetraacetic acid (EDTA). For the groups 6, 7, 8, and 9, the same solutions used in the groups 2, 3, 4, and 5 were used, respectively, but activated with ultrasonic activation. Afterwards, the roots were analyzed by a score considering the images obtained from a scanning electron microscope. Results: EDTA achieved the best performance compared with the other solutions evaluated regardless of the irrigation method (p < 0.05). Conclusions: Ultrasonic activation did not significantly influence smear layer removal.
Iatagan R. Josino,Bruno C. Martins,Andressa A. Machado,Gustavo R. de A. Lima,Martin A. C. Cordero,Amanda A. M. Pombo,Rubens A. A. Sallum,Ulysses Ribeiro Jr,Todd H. Baron,Fauze Maluf-Filho 대한소화기내시경학회 2023 Clinical Endoscopy Vol.56 No.6
Background/Aims: Self-expandable metallic stents (SEMSs) are widely adopted for the palliation of dysphagia in patients with malig-nant esophageal strictures. An important adverse event is the development of SEMS-induced esophagorespiratory fistulas (SEMS-ERFs). This study aimed to assess the risk factors related to the development of SEMS-ERF after SEMS placement in patients withesophageal cancer. Methods: This retrospective study was performed at the Instituto do Cancer do Estado de São Paulo. All patients with malignantesophageal strictures who underwent esophageal SEMS placement between 2009 and 2019 were included in the study. Results: Of the 335 patients, 37 (11.0%) developed SEMS-ERF, with a median time of 129 days after SEMS placement. Stent flare of 28mm (hazard ratio [HR], 2.05; 95% confidence interval [CI], 1.15–5.51; p=0.02) and post-stent chemotherapy (HR, 2.0; 95% CI, 1.01–4.00; p=0.05) were associated with an increased risk of developing SEMS-ERF, while lower-third tumors were a protective factor (HR,0.5; 95% CI, 0.26–0.85; p=0.01). No difference was observed in overall survival. Conclusions: The incidence of SEMS-ERFs was 11%, with a median time of 129 days after SEMS placement. Post-stent chemotherapyand a 28 mm stent flare were associated with a higher risk of SEMS-ERF.
Antimicrobial Activity of Polymeric Microfibers Containing Coix Lacryma-Jobi Extract
Gabriela Lauer Breitenbach,Marina Giombelli Rosenberger,Andressa Giombelli Rosenberger,Josiane Caetano,Michelly Cristina Galdioli Pellá,Desirée Tamara Scheidt,Cleide Viviane Buzanello Martins,Edvani C 한국고분자학회 2020 Macromolecular Research Vol.28 No.9
Wound dressings are based on the creation of ideal environments for cell mobility, gas exchanges and to promote tissue healing and regeneration, besides controlling bacterial proliferation. Electrospinning is a simple, cheap and common technique used for such purposes and Ecovio®, a polymeric blend based on biocompatible polymers (poly(butylene adipate-co-terephthalate) (PBAT) and poly(lactic acid) (PLA)), is a good example of polymer with unique properties for wound dressing materials development. To ensure antimicrobial properties, Job’s tears (Coix lacrymajobi) extract was incorporated into the material. The obtained fiber mats were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffractometry (DRX), optical microscopy (OM), and microbiological analysis. The interaction among the compounds of the extract and the polymeric matrix was inferred by the increase in the crystallinity degree and thermal stability of the fibers/extract. Also, a decrease in the diameter, roughness, and fiber homogeneity was observed. It happened due to the presence of extract in the fibers. Microbiological analysis (antimicrobial activity against S. aureus) indicated the effectiveness of the fibers/extract in preventing this bacteria growth, demonstrating the potential of this material for wound dressing purposes.
Cytostatic in vitro Effects of DTCM-Glutarimide on Bladder Carcinoma Cells
Brassesco, Maria S.,Pezuk, Julia A.,Morales, Andressa G.,De Oliveira, Jaqueline C.,Valera, Elvis T.,Da Silva, Glenda N.,De Oliveira, Harley F.,Scrideli, Carlos A.,Umezawa, Kazuo,Tone, Luiz G. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Bladder cancer is a common malignancy worldwide. Despite the increased use of cisplatin-based combination therapy, the outcomes for patients with advanced disease remain poor. Recently, altered activation of the PI3K/Akt/mTOR pathway has been associated with reduced patient survival and advanced stage of bladder cancer, making its upstream or downstream components attractive targets for therapeutic intervention. In the present study, we showed that treatment with DTCM-glutaramide, a piperidine that targets PDK1, results in reduced proliferation, diminished cell migration and G1 arrest in 5637 and T24 bladder carcinoma cells. Conversely, no apoptosis, necrosis or autophagy were detected after treatment, suggesting that reduced cell numbers in vitro are a result of diminished proliferation rather than cell death. Furthermore previous exposure to 10 ${\mu}g/ml$ DTCM-glutarimide sensitized both cell lines to ionizing radiation. Although more studies are needed to corroborate our findings, our results indicate that PDK1 may be useful as a therapeutic target to prevent progression and abnormal tissue dissemination of urothelial carcinomas.
Pressuring of acupoints as a complement to the stifle diseases diagnosis in horses
Luiza P.R. Mariani,Flávia Sampaio,Andressa B. Silveira,Luis F. Bastos,Saulo H. Weber,Pedro V. Michelotto 사단법인약침학회 2019 Journal of Acupuncture & Meridian Studies Vol.12 No.5
Diseases of the stifle joint remain a challenge for veterinarians. The objective of this study was to achieve a valuable acupuncture suggestive diagnosis to be considered for stifle joint diseases in horses. Thirty-nine nonlame horses involved in different activities were assessed. Acupuncture was independently performed by two evaluators. Reactions of the animal when pressurizing the point suggestive of stifle disease (PSSD), Bladder-20 and/or Bladder-21, were considered as the inclusion criteria for inclusion in the stifle group (SG, n Z 31), and the animals with no reactions were assigned to the control group (n Z 8). Radiographic and ultrasonographic examinations were performed and evaluated by two independent professionals blinded to the group allocation. Thermographic examination of the PSSD and stifles was also performed, after acclimatization. The ultrasound scores and radiographic findings were higher in the SG than in the control group. Thermography evidenced increased temperature in the PSSD and stifles in the SG. The minimum acupuncture diagnostic criteria for stifle joint disease had a sensitivity of 87.5% and a specificity of 57.0%, and the addition of the acupoints Gallbladderedorsal tuber coxae, Gallbladder-27, and Spleen-13 to the minimum diagnostic criteria improved sensitivity and specificity. In conclusion, assessing the reaction at the demonstrated acupoints can facilitate a diagnosis of a potential stifle lesion.