The Anticancer Role of Capsaicin in Experimentally-induced Lung Carcinogenesis

Anandakumar, Pandi,Kamaraj, Sattu,Jagan, Sundaram,Ramakrishnan, Gopalakrishnan,Asokkumar, Selvamani,Naveenkumar, Chandrashekar,Raghunandhakumar, Subramanian,Vanitha, Manickam Kalappan,Devaki, Thiruven KOREAN PHARMACOPUNCTURE INSTITUTE 2015 Journal of pharmacopuncture Vol.18 No.2

Objectives: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis. Methods: Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of $14^{th}$ week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson's trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out. Results: In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings. Conclusion: The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti-cancer role working against experimentally induced lung carcinogenesis.

Translocation of magnetic beads using patterned magnetic pathways for biosensing applications

Anandakumar, S.,Sudha Rani, V.,Jeong, J-R.,Kim, CheolGi,Kim, K. W.,Rao, B. Parvatheeswara American Institute of Physics 2009 JOURNAL OF APPLIED PHYSICS - Vol.105 No.7

<P>We have designed, fabricated, and demonstrated a novel system for translocation of magnetic beads at specific sites of the sensor surface on a single chip for biosensor applications. The soft NiFe elliptical (9 x 4 x 0.1 mu m(3)) elements are arranged as magnetic pathways connected to the model sensor surface. The patterned NiFe elements can generate different stray magnetic fields when they are subjected to the external rotating magnetic field. The inhomogeneity in stray magnetic fields can govern the magnetic bead motion on the pathways. We demonstrated the motion of Dynabead (R) M-280 magnetic bead on patterned pathways by controlling the external rotating magnetic field in clockwise and counterclockwise directions. The magnetic beads that were placed on the magnetic elliptical pathways are shown to be transported to the sensor surface, as well as be pulled out away from the surface. This technique enables microtranslocation of the magnetic beads coated with biomolecules to the specific binding sites of the sensor surface and as well as drive off the nonspecific binding biomolecules from the surface in performing number of sequential bead detection experiments for future integrated lab-on-a-chip systems. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3073965]</P>

Template Synthesis of Cobalt Nanowires Using PS-b-PMMA Block Copolymer

Anandakumar, S.,Sudha Rani, V.,Parvatheeswara Rao, B.,Seok Soo Yoon,Jeong, J.-R.,Cheol-Gi Kim IEEE 2009 IEEE transactions on magnetics Vol.45 No.10

<P>Cobalt nanowires with 18 plusmn 5 nm diameter were grown in the self-assembled diblock copolymer templates using electrodeposition technique. The diblock copolymer templates with hexagonally ordered nanoporous structure were successfully synthesized by varying the copolymer thickness from 50 nm to 430 nm and post annealing at temperature 180degC for 24 h. The minimum optimized pore sizes of the template were 18 plusmn 5 nm with well order hexagonal nanoporous structure at copolymer thickness from 350 nm to 430 nm. Cobalt nanowires were then successfully deposited using three electrode configuration potentiostatic electrodeposition methods. To remove the cross linked polystyrene of the template and to display the cobalt nanowires, heat treatment was carried out. The morphology of the templates and cobalt nanowires was observed using Scanning Electron Microscope (SEM). The magnetic properties of the cobalt nanowires are analyzed using Vibrating Sample Magnetometer (VSM).</P>

Ameliorating Effect of Capsaicin on Alterations in Lipid Metabolism during Mice Lung Carcinoma

P. Anandakumar,S. Jagan,S. Kamaraj,G. Ramakrishnan,J. Binu Clara,D. Pathitha,T. Kavitha,T. Devaki 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.2

Spices and vegetables possess antioxidant activity that can be applied for preservation of lipids and lower lipid peroxidation in biological systems. In the present study, we have investigated the effect of capsaicin on lipid metabolism during benzo(a)pyrene induced lung cancer in Swiss albino mice. Benzo(a)pyrene (50 mg/kg wt) induced lung cancer animals showed abnormal changes in the tissue and serum lipids, lipoproteins and lipid metabolizing enzymes. Treatment with capsaicin (10 mg/kg body wt) remarkably attenuated all the above alterations and restored normalcy. These findings reveal the chemomodulatory potential of capsaicin in attenuating the alterations in lipid metabolism during experimental lung carcinogenesis.

만성 및 급성 염증 모델에서 강황추출물의 효과

Senthilkumar Anandakumar,Joshua Allan Joseph,Bharathi Bethapudi,Amit Agarwal,정은봉(Eun-Bong Jung) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구에서는 강황 유래의 curcuminoids가 없는 다당체를 이용하여 급성 및 만성 염증 모델에서의 항염증 효과를 검증하고자 하였다. 급성염증에 대한 효과를 알아보기 위하여 carrageenan에 의한 족부종 유발과 xylene에 의한 귀부종 유발을 일으킨 모델을 이용하여 수행하였다. Carrageenan 에 의한 족부종 유발 실험에서 강황추출물과 관절염 치료제로 알려진 diclofenac sodium을 처리하였을 때는 부종의 증가도가 낮은 것으로 나타났다(P≤0.05). 또한 다른 급성염증 모델인 xylene application 마우스 모델에서도 대조군에 비하여 diclofenac sodium과 강황추출물(31.5, 63, 126 mg/kg)을 농도별로 경구투여 하였을 때 염증 억제율이 각각 78.13%, 68.75%, 62.50% 및 59.38%로 나타나 염증 억제율이 높은 것으로 나타났다(P≤0.05). 만성염증에 대한 효과를 알아보기 위한 cotton pellet 육아종 실험에서는 대조군과 비교하여 삼출물과 염증 육아종이 적게 발생된 것으로 나타났다(P≤0.05). 따라서 강황 유래의 다당체를 경구투여하였을 때 만성 및 급성 염증에 대한 저해활성이 있는 것으로 나타나 항염증 소재로서 개발 가능성이 있음을 제시하였다. Anti-inflammatory effects of turmeric (Curcuma longa L.) rich in polysaccharides, as well as free of curcuminoids and turmerones were investigated in acute and chronic inflammatory models. Activity against the acute phase of inflammation was evaluated in carrageenan-induced paw edema and xylene-induced ear edema models. The results showed that turmeric extract significantly decreased paw edema volume in the first and third hours after carrageenan injection (P≤0.05). Turmeric extract at all dose levels also significantly inhibited xylene-induced ear edema formation (P≤0.05). Activity against chronic inflammation was also evaluated in cotton pellet-induced granuloma model. Turmeric extract significantly (P≤0.05) decreased the weight of granuloma tissue on cotton pellets in a dose-dependent manner when compared to the vehicle control. Thus, the findings of the study suggest that turmeric extract in effective against both acute and chronic inflammation.

Translocation of Biomolecule Carriers Based on Smart Magnetophoresis

S. Anandakumar,V. Sudha Rani,Sun Jong Oh,B. L. Sinha,CheolGi Kim 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Special Lecture : Ultrasound and Screening for Chromosomal Abnormalities

( C. Anandakumar ) 대한산부인과학회 2002 대한산부인과학회 학술대회 Vol.88 No.-

Controllable Magnetic Bead Motion on Patterned Magnetic Pathways for Bio Sensing Applications

S. Anandakumar,V.Sudha Rani,Sunjong Oh,B.L.Sinha,J.-R. Jeong,CheolGi Kim 한국자기학회 2009 한국자기학회 학술연구발표회 논문개요집 Vol.2009 No.12

The Anticancer Role of Capsaicin in Experimentallyinduced Lung Carcinogenesis

Pandi Anandakumar,Sattu Kamaraj,Sundaram Jagan,Gopalakrishnan Ramakrishnan,Selvamani Asokkumar,Chandrashekar Naveenkumar,Subramanian Raghunandhakumar,Manickam Kalappan Vanitha,Thiruvengadam Devaki 대한약침학회 2015 Journal of pharmacopuncture Vol.18 No.2

Objectives: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis. Methods: Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of 14th week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson’s trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out. Results: In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson’s trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings. Conclusion: The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti- cancer role working against experimentally induced lung carcinogenesis.

[Retraction] A Review on the Role of Irisin in Insulin Resistance and Type 2 Diabetes Mellitus

Gizaw, Mamo,Anandakumar, Pandi,Debela, Tolessa KOREAN PHARMACOPUNCTURE INSTITUTE 2017 Journal of pharmacopuncture Vol.20 No.4

Irisin is a novel hormone like polypeptide that is cleaved and secreted by an unknown protease from fibronectin type III domain-containing protein 5 (FNDC5), a membrane-spanning protein and which is highly expressed in skeletal muscle, heart, adipose tissue, and liver. Since its discovery in 2012, it has been the subject of many researches due to its potent physiological role. It is believed that understanding irisin's function may be the key to comprehend many diseases and their development. Irisin is a myokine that leads to increased energy expenditure by stimulating the 'browning' of white adipose tissue. In the first description of this hormone, increased levels of circulating irisin, which is cleaved from its precursor fibronectin type III domain-containing protein 5, were associated with improved glucose homeostasis by reducing insulin resistance. Irisin is a powerful messenger, sending the signal to determine the function of specific cells, like skeletal muscle, liver, pancreas, heart, fat and the brain. The action of irisin on different targeted tissues or organs in human being has revealed its physiological functions for promoting health or executing the regulation of variety of metabolic diseases. Numerous studies focus on the association of irisin with metabolic diseases which has gained great interest as a potential new target to combat type 2 diabetes mellitus and insulin resistance. Irisin is found to improve insulin resistance and type 2 diabetes by increasing sensitization of the insulin receptor in skeletal muscle and heart by improving hepatic glucose and lipid metabolism, promoting pancreatic ${\beta}$ cell functions, and transforming white adipose tissue to brown adipose tissue. This review is a thoughtful attempt to summarize the current knowledge of irisin and its effective role in mediating metabolic dysfunctions in insulin resistance and type 2 diabetes mellitus.