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An-Na Li,Chen Xie,Jie Zhang,Jia Zhang,Duo-Chuan Li 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
The serine protease gene from a thermophilic fungus Thermoascus aurantiacus var. levisporus, was cloned,sequenced, and expressed in Pichia pastoris and the recombinant protein was characterized. The full-length cDNA of 2,592 bp contains an ORF of 1,482 bp encoding 494 amino acids. Sequence analysis of the deduced amino acid sequence revealed high homology with subtilisin serine proteases. The putative enzyme contained catalytic domain with active sites formed by three residues of Asp183, His215, and Ser384. The molecular mass of the recombinant enzyme was estimated to be 59.1 kDa after overexpression in P. pastoris. The activity of recombinant protein was 115.58 U/mg. The protease exhibited its maximal activity at 50°C and pH 8.0 and kept thermostable at 60°C, and retained 60% activity after 60 min at 70°C. The protease activity was found to be inhibited by PMSF, but not by DTT or EDTA. The enzyme has broad substrate specificity such as gelatin, casein and pure milk, and exhibiting highest activity towards casein.
Yanxia Li,Liya Yu,Jun Na,Shuang Li,Li Liu,Huijuan Mu,Xuanjuan Bi,Xiaoxia An,Xun Li,Wen Dong,Guowei Pan 대한암학회 2017 Cancer Research and Treatment Vol.49 No.4
Purpose The cancer survival was characterized by following up sampled subgroups of cancer cases from three population-based cancer registries in Northeast China. Materials and Methods Survival analysis was used to analyze 6,871 patients, who had one of the 21 most common cancers based on sampling from the population-based cancer registries of three cities in Liaoning Province. All patients were diagnosed between 2000 and 2002 and were followed up to the end of 2007 by active and passive methods. The 5-year age standardized relative survival rates (ASRS) were estimated for all cancers combined and each of the 21 individual cancers. Results The survival status was traced for 80.8% of 8,506 sampled cancer cases. The 5-year ASRS for all 21 cancers combined was 41.5% (95% confidence interval, 40.3 to 42.7), the highest ASRS was observed for thyroid cancer (85.2%), breast cancer (78.9%), uterine corpus cancer (75.9%), and urinary bladder cancer (70.2%); the lowest 5-year ASRS was noted in pancreatic cancer (8.8%), liver cancer (11.0%), esophageal cancer (18.8), and lung cancer (19.6%). The cancer survival rates in Liaoning cities were similar to those of urban areas in mainland China, but significantly lower than those in Hong Kong, Korea, and Japan. Conclusion The strikingly poor cancer survival rates in three cities of Liaoning Province and in other places in China highlight the need for urgent investment in cancer prevention, early detection, and standardized and centralized treatment.
Chen, An-He,Chai, You-Rong,Li, Jia-Na,Chen, Li Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.2
Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.
The anti-diabetic drug dapagliflozin induces vasodilation via activation of PKG and Kv channels
Li, Hongliang,Shin, Sung Eun,Seo, Mi Seon,An, Jin Ryeol,Choi, Il-Whan,Jung, Won-Kyo,Firth, Amy L.,Lee, Dae-Sung,Yim, Mi-Jin,Choi, Grace,Lee, Jeong Min,Na, Sung Hun,Park, Won Sun Elsevier 2018 Life sciences Vol.197 No.-
<P><B>Abstract</B></P> <P><B>Aim</B></P> <P>Considering the clinical efficacy of dapagliflozin in patients with type 2 DM and the pathophysiological relevance of Kv channels for vascular reactivity. We investigate the vasodilatory effect of dapagliflozin and related mechanisms using phenylephrine (Phe)-induced contracted aortic rings.</P> <P><B>Material and methods</B></P> <P>Arterial tone measurement was performed in aortic smooth muscle.</P> <P><B>Key findings</B></P> <P>Application of dapagliflozin induced vasodilation in a concentration-dependent manner. Pre-treatment with the BK<SUB>Ca</SUB> channel inhibitor paxilline, the K<SUB>ATP</SUB> channel inhibitor glibenclamide, and the Kir channel inhibitor Ba<SUP>2+</SUP> did not change dapagliflozin-induced vasodilation. However, application of the Kv channels inhibitor 4-AP effectively inhibited dapagliflozin-induced vasodilation. Application of the Ca<SUP>2+</SUP> channel inhibitor nifedipine and the sarcoplasmic/endoplasmic reticulum Ca<SUP>2+</SUP>-ATPase (SERCA) pump inhibitor thapsigargin did not alter the vasodilatory effect of dapagliflozin. Moreover, the adenylyl cyclase inhibitor SQ 22536 and the protein kinase A (PKA) inhibitor KT 5720 had no effect on dapagliflozin-induced vasodilation. Although guanylyl cyclase inhibitors, NS 2028 and ODQ, did not reduce the vasodilatory effect of dapagliflozin, the protein kinase G (PKG) inhibitor KT 5823 effectively inhibited dapagliflozin-induced vasodilation. The vasodilatory effect of dapagliflozin was not affected by elimination of the endothelium. Furthermore, pretreatment with the nitric oxide synthase inhibitor L-NAME or the small-conductance Ca<SUP>2+</SUP>-activated K (SKCa) channel inhibitor apamin did not change the vasodilatory effect of dapagliflozin.</P> <P><B>Significance</B></P> <P>We concluded that dapagliflozin induced vasodilation via the activation of Kv channels and PKG, and was independent of other K<SUP>+</SUP> channels, Ca<SUP>2+</SUP> channels, intracellular Ca<SUP>2+</SUP>, and the endothelium.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
심포지움,특별강연 및 일반연제 발표 : 신증후군 환자에서 알부민 전처치가 Furosemide 이뇨효과에 미치는 약력학 및 약동학적 유용성 평가
나기영 ( Na Gi Yeong ),김강석 ( Kim Gang Seog ),김연수 ( Kim Yeon Su ),안규리 ( An Gyu Li ),한진석 ( Han Jin Seog ),김성권 ( Kim Seong Gwon ),이정상 ( Lee Jeong Sang ),배균섭 ( Bae Gyun Seob ),신상구 ( Sin Sang Gu ),허우성 ( Heo U 대한신장학회 2000 춘계학술대회 초록집 Vol.19 No.2